上海軒澤康生物秉承誠(chéng)信原則：人B/T-淋巴細(xì)胞衰減因子(BTLA)ELISA試劑盒實(shí)驗(yàn)全程提供技術(shù)指導(dǎo)（從售前的標(biāo)本收集到實(shí)驗(yàn)結(jié)束后的數(shù)據(jù)分析），試劑盒有任何質(zhì)量問(wèn)題上海軒澤康生物包退包換。同時(shí)我司承諾凡訂購(gòu)任意一款ELISA檢測(cè)試劑盒，將為您免費(fèi)代測(cè)，為您節(jié)省時(shí)間。您只需將標(biāo)本寄到我司，實(shí)驗(yàn)結(jié)果、原始數(shù)據(jù)、分析數(shù)據(jù)均可提供。

一.   樣本的準(zhǔn)備和保存：如果不立即分析，應(yīng)分裝后冷凍保存，且避免反復(fù)凍融。

【血清】：使用不含熱原和內(nèi)毒素的試管，操作過(guò)程中避免任何細(xì)胞 刺激，收集血液后，3000 轉(zhuǎn)離心 10 分鐘將血清和紅細(xì)胞迅速小心地 分離。

【血漿】：EDTA、檸檬酸鹽或肝素抗凝。3000 轉(zhuǎn)離心 30 分鐘取上清。

【細(xì)胞上清液】：3000 轉(zhuǎn)離心 10 分鐘去除顆粒和聚合物。

【組織勻漿】：將組織加入適量生理鹽水搗碎。3000 轉(zhuǎn)離心 10 分鐘 取上清。

保存：如果樣本收集后不及時(shí)檢測(cè)，請(qǐng)按一次用量分裝，凍存于 -20℃，避免反復(fù)凍融，在室溫下解凍并確保樣品均勻地充分解凍。

二.   人B/T-淋巴細(xì)胞衰減因子(BTLA)ELISA試劑盒組成：（標(biāo)準(zhǔn)品濃度依次為：0、50、100、200、400、800 ng/L）

1. 問(wèn)：是否可以使用不同試劑盒的組分？

上海軒澤康生物解答：每一個(gè)試劑盒包含具有特定批號(hào)的組件，以確保所有組件單獨(dú)以及試劑盒中所有其他組件都能達(dá)到最佳性能。對(duì)這些特定批次進(jìn)行質(zhì)量控制測(cè)試。絕不建議用戶(hù)使用自己的組件或其他供應(yīng)商試劑盒。
Q:Can components from different kits be used?
A:Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

2. 問(wèn)：我的標(biāo)準(zhǔn)曲線(xiàn)看起來(lái)很好，但我沒(méi)有在樣本中得到預(yù)期的信號(hào)，這是為什么？

上海軒澤康生物解答：樣品可能不含分析物。矩陣效應(yīng)可能掩蓋檢測(cè)。確保按照我司試劑盒中說(shuō)明書(shū)進(jìn)行稀釋。稀釋?zhuān)罢?qǐng)檢查以確保稀釋操作正確。過(guò)度稀釋可能導(dǎo)致樣品低于標(biāo)準(zhǔn)曲線(xiàn)的范圍。
Q:My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?
A:The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

3. 問(wèn)：我該如何洗板？

上海軒澤康生物解答：如果您使用的是自動(dòng)洗板機(jī)，我們建議定期檢查校正，并在清洗前用洗板機(jī)緩沖液沖洗系統(tǒng)。手動(dòng)洗板機(jī)也是如此。或者使用中繼器或洗滌瓶。用戶(hù)應(yīng)小心確保所有內(nèi)容物都被吸入，并在無(wú)棉紙上輕敲干燥。
Q:How do you recommend I wash my plate?
A:If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, and that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated and the plate tapped dry on lint-free paper.