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玩具傳播流感病毒診斷試劑（酶聯(lián)免疫法）

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玩具傳播流感病毒診斷試劑（酶聯(lián)免疫法）

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】     歐

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

鑲嵌蛋白和它周圍的脂類分子形成晶格狀態(tài)，這些不移動(dòng)的脂類分子稱界面脂質(zhì)，而流動(dòng)的脂質(zhì)呈小片、點(diǎn)狀分布。所以脂質(zhì)的流動(dòng)是局部的，并非整個(gè)脂雙層都在流動(dòng)。[3] 
板塊鑲嵌模型
1977年，Jain和White提出生物膜是由具有不同流動(dòng)性的板塊鑲嵌而成的動(dòng)態(tài)結(jié)構(gòu)。[3] 
脂筏模型
脂筏（lipid raft）是質(zhì)膜上富含膽固醇和鞘磷脂的微結(jié)構(gòu)域（microdomain）。大小約70nm左右，是一種動(dòng)態(tài)結(jié)構(gòu)，位于質(zhì)膜的外小頁。由于鞘磷脂具有較長的飽和脂肪酸鏈，分子間的作用力較強(qiáng)，所以這些區(qū)域結(jié)構(gòu)致密，介于無序液體與液晶之間，稱為有序液體（Liquid-ordered）。在低溫下這些區(qū)域能抵抗非離子去垢劑的抽提，所以又稱為抗去垢劑膜（detergent-resistant membranes，DRMs）。脂筏就像一個(gè)蛋白質(zhì)停泊的平臺(tái)，與膜的信號(hào)轉(zhuǎn)導(dǎo)、蛋白質(zhì)分選均有密切的關(guān)系。
從脂筏的角度來看，膜蛋白可以分為三類：①存在于脂筏中的蛋白質(zhì)；包括糖磷脂酰肌醇錨定蛋白（GPI anchored protein），某些跨膜蛋白，Hedgehog蛋白，雙乙?；鞍祝╠oubly acylated protein）如：非受體酪氨酸激酶Src、G蛋白的Gα亞基、血管內(nèi)皮細(xì)胞的一氧化氮合酶（NOS）；②存在于脂筏之外無序液相的蛋白質(zhì)；③介于兩者之間的蛋白質(zhì)，如某些蛋白在沒有接受到配體時(shí)，對(duì)脂筏的親和力低，當(dāng)結(jié)合配體，發(fā)生寡聚化時(shí)就會(huì)轉(zhuǎn)移到脂筏中。
脂筏中的膽固醇就像膠水一樣，它對(duì)具有飽和脂肪酸鏈的鞘磷脂親和力很高，而對(duì)不飽和脂肪酸鏈的親和力低，用甲基-β-環(huán)糊精（methyl-β-cyclodextrin）去除膽固醇，抗去垢劑的蛋白就變得易于提取。膜中的鞘磷脂主要位于外小頁，而且大部分都參與形成脂筏。
據(jù)估計(jì)脂筏的面積可能占膜表面積的一半以上。脂筏的大小是可以調(diào)節(jié)的，小的獨(dú)立脂筏可能在保持信號(hào)蛋白呈關(guān)閉狀態(tài)方面具有重要作用，當(dāng)必要時(shí)，這些小的脂筏聚集成大一個(gè)大的平臺(tái)，在那里信號(hào)分子（如受體）將和它們的配件相遇，啟動(dòng)信號(hào)傳遞途徑。如致敏原（allergen）能夠?qū)⑦^敏患者體內(nèi)肥大細(xì)胞或嗜堿性細(xì)胞表面的IgE抗體及其受體橋聯(lián)起來，形成較大的脂筏，受體被脂筏中的Lyn（一種非受體酪氨酸激酶)磷酸化，啟動(dòng)下游的信號(hào)轉(zhuǎn)導(dǎo)，zui終引發(fā)過敏反應(yīng)。

The inlaid protein and its surrounding lipid molecules form a lattice state. These non-moving lipid molecules are called interfacial lipids, and the flowing lipids are small and dotted. So the flow of lipids is local, not the entire lipid bilayer is flowing. [3]
Plate mosaic model
In 1977, Jain and White proposed that biofilms are dynamic structures inlaid with plates of different mobility. [3]
Lipid raft model
Lipid rafts are microdomain rich in cholesterol and sphingomyelin on the plasma membrane. About the size of about 70nm, is a dynamic structure, located in the outer membrane of the small pages. Because sphingomyelin has a long saturated fatty acid chain, strong intermolecular forces, so dense structure of these regions, between the liquid and the liquid disorder between the order, called the liquid (Liquid-ordered). These areas are resistant to extraction of non-ionic detergents at low temperatures and are also known as detergent-resistant membranes (DRMs). Lipid raft is like a protein docking platform, and the membrane signal transduction, protein sorting are closely related.
From the perspective of lipid rafts, membrane proteins can be divided into three categories: ① proteins present in lipid rafts; including GPI anchored proteins, certain transmembrane proteins, Hedgehog proteins, Doubly acylated proteins such as non-receptor tyrosine kinase Src, Gα subunit of G protein and nitric oxide synthase (NOS) of vascular endothelial cells; ② exist in the liquid phase outside the lipid rafts Of proteins; ③ between the two proteins, such as some proteins in the absence of ligand received, the low affinity of the lipid raft, when the ligand, when the oligomerization will be transferred to the lipid raft.
Cholesterol in lipid rafts acts like glue, which has high affinity for sphingomyelins with saturated fatty acid chains and low affinity for unsaturated fatty acid chains, is removed with methyl-β-cyclodextrin Cholesterol, anti-detergent protein becomes easy to extract. Sphingomyelin in the membrane is mainly located on the outer small pages, and most of them are involved in the formation of lipid rafts.
It is estimated that the area of ??lipid rafts may account for more than half of the membrane surface area. The size of the lipid rafts is regulatable, and small, independent lipid rafts may play an important role in keeping the signaling proteins in a closed state. When necessary, these small lipid rafts aggregate into a large, large platform where signal molecules, such as Receptor) will meet with their accessory to initiate the signaling pathway. Such as allergen (allergen) can allergic patients with mast cells or basophil IgE antibodies and their receptors on the bridge to form a larger lipid raft, the receptor lipid raft Lyn (a non Receptor tyrosine kinase) phosphorylation, start downstream signal transduction, eventually triggering an allergic reaction.