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【公司名稱】 廣州健侖生物科技有限公司
【】    楊永漢 
【】 
【騰訊 】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-3室
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因與肺吸蟲及中華支睪吸蟲有交叉反應，又因尾蚴供應困難，故不易推廣，僅用于流行病學調查及篩選新感染者。3．環(huán)卵沉淀試驗 取活卵懸液一滴于無菌玻片上，加病人血清等量，加蓋玻片石臘密封，置37℃溫孵24～48小時，于低倍鏡下觀察，可見蟲卵周圍出現(xiàn)球狀、指狀、絲狀、菊花狀等形態(tài)的沉淀物。觀察100個成熟蟲卵，計算沉淀物大于10微米的蟲卵數(shù)所占的百分數(shù)，環(huán)沉率5%以上者為陽性。感染后7～12天出現(xiàn)反應，陽性率達95%以上，具有早期診斷價值。4．間接血凝試驗 采用血吸蟲卵抗原致敏紅細胞測定病人血清中的抗體，明顯凝集者為陽性，特異性與敏感性高，陽性率在90%以上，觀察結果快，用血量少及操作簡便，本試驗與肺吸蟲有交叉反應。5．酶聯(lián)免疫吸附試驗（ELISA） 以純化成蟲或蟲卵抗原與過氧化物酶或堿性磷酸酶結合，測定病人血清或尿中的血吸蟲抗體，敏感性及特異性高，陽性率在95%以上，操作簡便，適用于大規(guī)模現(xiàn)場使用。6．酶聯(lián)免疫電泳試驗 過氧化物酶標記純化蟲卵抗原后，作對流免疫電泳，檢測血清特異性抗體，1/2～1小時出報告，陽性率80%以上。7．循環(huán)抗原測定 循環(huán)抗原是存在于病人血與尿中的特異性抗原物質，循環(huán)抗原陽性，說明宿主體內存在有活的血吸蟲感染，具有考核藥物療效的價值。5．腸鏡檢查及腸粘膜活組織檢查 疑似血吸蟲的而反復大便檢查蟲卵性者適用腸鏡檢查?？梢娔c粘膜有黃斑、息肉、瘢痕、肥厚、充血、水腫、潰湯等改變。自蟲卵堆積處（黃斑及粘膜增厚處）取米粒大小的粘膜組織壓片，在顯微鏡下檢查，可查到成堆的蟲卵，陽性率在90%以上；在直腸粘膜活檢中所見蟲卵多成黑色死卵與空卵殼，活卵較少。為此，僅憑活檢出卵對近期變性蟲不易區(qū)別，又因死卵于治療后可長期留在腸壁中，久不消失，故認為對療效考核只能作參考?；顧z時應充分止血，輕取組織、切忌撕拉，以防止出血與穿孔。1．血象 急性期白細胞總數(shù)及嗜酸性粒細胞顯著增多，白細胞總數(shù)多在10～30×109/L，嗜酸性細胞常占20～40%或更高，有時可達80%以上。部分病人嗜酸性粒細胞增高不顯著。慢性期嗜酸性粒細胞輕度增多。晚期因脾功能進，白細胞明顯減少，并伴有貧血及血小板減少。2．肝功能試驗 急性期多因蟲卵激導致網(wǎng)狀內皮細胞增生

Due to cross-reaction with Pneumocystis and Clonorchis sinensis, it is difficult to popularize because of the difficulty in supplying tail lice. It is only used for epidemiological investigation and screening for new infections. 3. Cyclic egg sedimentation test to take a drop of live egg suspension on a sterile glass slide, add the patient's serum equal amount, cover glass slides, sealed with paraffin, and incubate at 37°C for 24 to 48 hours. Observe under low power microscope, visible around the eggs There are spherical, finger-like, filamentous, chrysanthemum-like precipitates. Observe 100 mature eggs, and calculate the percentage of eggs with sediments larger than 10 μm, and the positive rate is 5% or more. After 7 to 12 days of infection, a positive reaction rate of more than 95% appeared to be of early diagnostic value. 4. Indirect hemagglutination test using schistosomiasis antigen-sensitized red blood cells to determine the antibody in the patient's serum, obvious agglutination was positive, high specificity and sensitivity, the positive rate of more than 90%, observation results fast, with less blood and easy to operate, This test cross-reacted with paragonimiasis. 5. Enzyme-Linked Immunosorbent Assay (ELISA) The detection of schistosomiasis antibodies in serum or urine of patients with purified adult or egg antigen combined with peroxidase or alkaline phosphatase has high sensitivity and specificity, and the positive rate is above 95%. Easy to operate, suitable for large-scale field use. 6. Enzyme-linked immunoelectrophoresis assay After peroxidase-labeled purification of egg antigens, counter-current immunoelectrophoresis was performed to detect serum-specific antibodies. Reports were made 1/2 to 1 hour, and the positive rate was over 80%. 7. Cyclic antigen determination Cyclic antigen is a specific antigen present in the patient's blood and urine, circulating antigen positive, indicating that there is a live schistosome infection in the host body, which has the value of assessing drug efficacy. 5. Colonoscopy and intestinal mucosa biopsy Suspected schistosomiasis and repeated bowel examination of eggs for colonoscopy. Visible intestinal mucosa with macular, polyps, scars, hypertrophy, congestion, edema, ulceration and other changes. Accumulation of mucosal-sized mucous membranes from the site where the eggs were deposited (macular and mucous membrane thickening). Examination under the microscope can reveal piles of eggs, with a positive rate of more than 90%; seen in rectal mucosa biopsies. Eggs are mostly black dead eggs and empty eggshells, with few live eggs. For this reason, it is not easy to differentiate the newly-depopulated worms by the biopsy of eggs, and because the dead eggs can remain in the intestinal wall for a long period of time after treatment, it will not disappear for a long time. Therefore, it is considered that the evaluation of the efficacy can only be used as a reference. The biopsy should be fully stopped bleeding, gentle tissue, avoid tearing to prevent bleeding and perforation. 1. The total number of white blood cells and eosinophils in the acute phase of blood is significantly increased. The total number of leukocytes is usually 10 to 30×10 9 /L. Eosinophils often account for 20 to 40% or more, and sometimes reach 80% or more. Elevation of eosinophils was not significant in some patients. Chronic mild eosinophils increased. Due to the advance of spleen function in the late stage, the white blood cells are significantly reduced, accompanied by anemia and thrombocytopenia. 2. Liver function test: In the acute phase, multiple worm eggs stimulate reticuloendothelial cell proliferation.