Reversed-Phase and Weak Anion-Exchange Properties on a Single Column

The novel column chemistry of the Acclaim^® Mixed-Mode WAX-1 results in a multimode separation mechanism including hydrophobic, anion-exchange, and cation-exclusion interactions. Consequently, retention of basic, neutral, and acidic molecules can be either independently or concurrently adjusted by changing ionic strength, pH, and organic solvent content in the phase.

• Adjustable selectivity

• Selectivity orthogonal to reversed-phase (RP) columns

• Ideal selectivity for anionic molecules

• Simultaneous separation of acidic, basic, and neutral molecules

• Multimode retention mechanisms: reversed- and normal phase, anion-exchange, cation-exclusion, and HILIC modes

The Acclaim Mixed-Mode WAX-1 column combines hydrophobic and anion-exchange characteristics, on a 5 μm high-purity, porous silica with 120-Å diameter pores. Because of this unique chemistry, the Acclaim Mixed-Mode WAX-1 column also provides complimentary selectivity to C18 reversed-phase columns.

Hydrophilic organic acids are often difficult to separate with common RP columns, since hydrophobic retention alone is inadequate to differentiate molecules with similar hydrophobicities. This can be overcome using the Acclaim Mixed-Mode WAX-1 column; it provides sufficient retention, and ideal selectivity for a variety of anionic molecules, even for weakly charged molecules.

Multi-Mode Separation Mechanism

Retention of basic, neutral and acidic molecules can be either independently or concurrently adjusted by changing ionic strength, pH, and organic solvents content in the phase. The presence of anion-exchange functionality control through electrostatic attraction (anions), repulsion (cations). The alkyl chain controls separation by hydrophobic interaction.

A broad range of samples in the pharmaceutical, food and beverage and chemical industries can be separated. Separation of basic active ingredients and its counter-ion can be simultaneously determined, as well as separation of ingredients containing basic, neutral, and acidic ingredients.