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Polymeric Reversed-Phase Column Ideal for the Separation of Hydrophobic, Ionizable Compounds
The IonPac® NS1-10μm and NS1-5μm columns are packed with a neutral, macroporous, high-surface-area, ethylvinylbenzene polymer crosslinked with 55% divinylbenzene. This resin makes the NS1 resistant to solvents, acids, and bases, and permits the use of eluent from pH 0 to 14.
Ideal for separation of large molecules that carry localized charges, such as surfactants
Compatible with acids, bases, and solvent from pH 0 to 14
Can also be used for traditional polymeric reversed-phase applications
Utilize ion-pair chromatography for difficult separations
The IonPac NS1 column is the column of choice for routine ion-pair chromatography or ion-suppression separations using theAMMS® ICE 300 suppressor. The NS1 is also used with phase ion chromatography (MPIC®), a technique especially suited to large molecules that carry localized charges (e.g., surfactants), as well as other ions that are not amenable to separation by ion-exchange chromatography.
Key Applications
The IonPac NS1 column can be used for the separation of high MW aliphatic carboxylic acids, alkyl and aromatic sulfates and sulfonates, quaternary ammonium ions, water-soluble vitamins, sulfur oxides, metal cyanide complexes, phenols, alkanolamines.
| IonPac NS1 Polymeric Reversed-Phase Column Specifications | |
|---|---|
| Dimensions | IonPac NS1-10µm Analytical Column: 4 × 250 mm IonPac NS1-10µm Guard Column: 4 × 35 mm IonPac NS1-5µm Analytical Column: 4 × 150 mm |
| Maximum operating pressure | <4000 psi (<27.6 MPa) |
| Typical operating conditions | Gradient: 1.0 mL/min Recommended Operating Temperature: Ambient Recommended Flow Rate: 1.0 mL/min Typical Ion-Pairing Reagents: For anions: ammonium and tetramethyl-, tetraethyl-, tetrapropyl-, and tetrabutyl-ammonium hydroxides. For cations: hydrochloric, perchloric, and perfluorocarboxylic acids; pentane-, hexande-, heptane-, and octanesulfonic acids. |
| phase compatibility | pH 0–14; 1–* HPLC solvents |
| Resin composition | Bead Diameter: 10 µm (4 × 250 mm), 5 µm (4 × 150 mm), macroporous ethylvinylbenzene/divinylbenzene resin Cross-Linking (%DVB): 55% Hydrophobicity: High |
| Capacity | 60 µeq (2 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |