(Dap²²)-ShK (Stichodactyla toxin) 

Selective blocker of K_v1.3(鉀離子通道K_v1.3選擇性阻斷劑)

(Dap²²)-ShK (ShK Dap22) peptide is a synthetic derivative of the well-known ShK toxin #08SHK001 (Stichodactyla helianthus neurotoxin) isolated from the venom of the Carribean sea anemone Stoichactis helianthus. Wild-type ShK blocks potently  K_v1.3 (KCNA3), K_v1.1 (KCNA1), K_v1.4 (KCNA4), and K_v1.6 (KCNA6) respectively with a Kd of 11 pM,16 pM, 312 pM and 165 pM. In (Dap²²)-ShK, lysine²² has been replaced by a diaminopropionic acid (Dap) residue that greatly improves the selectivity of the peptide for the voltage-gated potassium channel K_v1.3 (IC₅₀ around 23 pM) against K_v1.1 (1.8 nM), K_v1.4 (37 nM) and K_v1.6 (10 nM) channels. The high selectivity of (Dap22)-Shk is achieved thanks to the strong binding between the Dap and His404 / Asp386 residues of K_v1.3 channel. (Dap²²)-ShK inhibits T cell proliferation induced by anti-CD3 at subnanomolar concentrations.

(Dap²²)-ShK 技術(shù)資料

氨基酸序列：

Arg-Ser-Cys³-Ile-Asp-Thr-Ile-Pro-Lys-Ser-Arg-Cys¹²-Thr-Ala-Phe-Gln-Cys¹⁷-Lys-His-Ser-Met-Dap-Tyr-Arg-Leu-Ser-Phe-Cys²⁸-Arg-Lys-Thr-Cys³²-Gly-Thr-Cys³⁵-OH

二硫鍵： Cys³-Cys³⁵, Cys¹²-Cys²⁸ and Cys¹⁷-Cys³² 

長度 (aa)：35

分子式：C₁₆₆H₂₆₈N₅₄O₄₈S₇ 

分子量：4012.8 Da

外觀：White lyophilized solid

可溶性： water and saline buffer

CAS 號：220384-25-8

來源： Synthetic

純度： > 97%

產(chǎn)品信息