廣州測(cè)迪生物科技有限公司
主營(yíng)產(chǎn)品: 美國(guó)kernel試劑盒,德國(guó)拜發(fā)試劑盒,瑞士Prionics試劑盒,荷蘭賽迪試劑盒 |
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廣州測(cè)迪生物科技有限公司
主營(yíng)產(chǎn)品: 美國(guó)kernel試劑盒,德國(guó)拜發(fā)試劑盒,瑞士Prionics試劑盒,荷蘭賽迪試劑盒 |
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豬繁殖與呼吸綜合征病毒抗體檢測(cè)試劑盒
名稱(chēng)和用途
Prionics 藍(lán)耳病 豬繁殖與呼吸綜合征抗體檢測(cè)試劑盒是用于檢測(cè)豬血清和血漿中的豬繁殖與呼吸綜合征病毒(PRRSV)抗體的酶聯(lián)免疫檢測(cè)試劑盒。
概述
豬繁殖與呼吸綜合征病毒(PRRSV)是一種動(dòng)脈炎病毒,在80 年代后期*在歐洲和美國(guó)發(fā)現(xiàn),兩種毒株具有不同的抗原性。該病毒引起繁殖問(wèn)題( 流產(chǎn),產(chǎn)弱仔,死胎, 母豬死亡)、呼吸系統(tǒng)疾?。ǚ窝祝粑щy,行動(dòng)遲緩,死亡)和輕度神經(jīng)癥狀1。豬繁殖與呼吸綜合征引起嚴(yán)重的經(jīng)濟(jì)損失,被認(rèn)為是影響?zhàn)B豬業(yè)zui重要的疾病之一。可以通過(guò)便捷的檢測(cè)血清或血漿中的抗體評(píng)估豬只是否感染PRRSV(無(wú)論是自然感染或人工免疫疫苗)。需要注意的是這種檢測(cè)抗體的免疫學(xué)方法需要對(duì)不同的PRRS 毒株的檢測(cè)都很敏感。
此試劑盒僅供體外檢測(cè)使用。
原理
Prionics試劑盒通過(guò)*的抗體設(shè)計(jì),能同時(shí)檢測(cè)美洲株和歐洲株不同血清型的藍(lán)耳病毒。將重組的ORF7蛋白 (美洲株和歐洲株)包被在微孔滴定板上,被測(cè)樣品在包被孔中孵育時(shí),針對(duì)PRRSV的特異性抗體與包被的抗原形成抗原抗體復(fù)合物,洗去包被孔中沒(méi)有結(jié)合的成分后,加入辣根過(guò)氧化物酶(HRPO)標(biāo)記的抗豬抗體,它與孔中的豬抗體結(jié)合,在試驗(yàn)的zui后步驟,洗去沒(méi)有結(jié)合的酶標(biāo)抗體,再加入酶底物和顯色劑TMB,顏色反應(yīng)的深淺由樣品中存在的抗PRRSV 特異性抗體的量決定。
The PrioCHECK? PRRSV Antibody ELISA is specially designed for the detection of both the American and the European genotypes of the PRRS virus in serum samples. Plates are coated with ORF 7 protein (common to both genotypes) ensuring the detection of antibodies to both virus types with high sensitivity and specificity. The PrioCHECK? PRRSV Antibody ELISA shows a high agreement with the most frequently used ELISA in the field and is therefore the most powerful alternative on the PRRS test market.
Test procedure
The PrioCHECK? PRRSV Antibody ELISA is a diagnostic kit based on ELISA technology. It follows a simple four step protocol consisting of sample preparation, sample incubation, conjugate incubation, and detection. Easy handling makes the test convenient and fast: 90 samples can be analyzed within 150 minutes. Serum samples are incubated in an ELISA plate coated with the ORF 7 protein of PRRSV. Anti-PRRSV antibodies present in the sample bind to the antigen. Plates are then washed and an anti-swine antibody labeled with horse radish peroxidase (HRPO) is used to detect antibodies bound to the ORF 7 coated plate. Color development using tetramethyl-benzidine (TMB) substrate measured optically at a wavelength of 450 nm shows the presence of antibodies directed against PRRSV.