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產品名稱：Invitrogen 18012021 LARGE FRAGMENT
貨 號：18012021
規(guī) 格：100 UN
分 類：DNA/RNA 聚合酶
品 牌：Invitrogen
國產/進口：進 口
儲存溫度：-20°C
特惠價格：致 電
庫存狀態(tài)：現(xiàn) 貨
銷售商：上?；鄯f生物科技有限公司
慧穎生物專業(yè)供應Invitrogen 18012021 LARGE FRAGMENT，常備現(xiàn)貨，*。主營Life Invitrogen產品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。
描述：
DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities.
Applications:
Fill-in of 5′ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.
Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.
Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.
Unit Definition:
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template?primer.
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