系統(tǒng)功能靈活,適合多種突變檢測方法
在癌癥研究發(fā)展及多種單基因或多基因疾病的研究過程中,探索未知突變起著非常重要的作用。DNA 多態(tài)性 是物種的生態(tài)與進(jìn)化研究非常有用的工具。應(yīng)用范圍包括物種鑒定、種群結(jié)構(gòu)分析及遺傳多樣性研究等。
DCode 系統(tǒng)由帶溫度控制模塊(包括微處理器控制加熱)的電泳設(shè)備、一個緩沖液再循環(huán)泵、一個攪拌器及鋪制特殊應(yīng)用凝膠的附件組成。冷卻槽及一個外部實驗室冷卻器可把運行溫度控制在室溫下。一塊凝膠能在2 個小 時內(nèi)運行64 個樣品,并提供5-70°C 范圍內(nèi)的精確溫度控制。它可與下列的電泳技術(shù)配合使用來檢測單堿基變化:
單鏈構(gòu)象多態(tài)性(SSCP)
變性梯度凝膠電泳(DGGE)
時間溫度梯度電泳(TTGE)
均一變性凝膠電泳(CDGE)
為了獲得zui高的通量和效率,許多實驗室采用多種技術(shù)。在快節(jié)奏研究環(huán)境中,不同方法間的快速切換和轉(zhuǎn)換 到新技術(shù)的能力是非常關(guān)鍵的。DCode 系統(tǒng)能迎合所有主要突變檢測技術(shù)的需要。其靈活的功能與優(yōu)點包括:
溫度控制選擇
模塊式設(shè)計,可滿足用戶現(xiàn)在與將來的需要
的組合配件,簡化啟動過程
針對特定技術(shù)的試劑和對照物,適合DGGE、CDGE、SSCP 和TTGE 等技術(shù)目錄 #
描述
DCode Systems
170-9080
DCode System for DGGE, 120 V, for 16 cm gels with single prep well (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9081
DCode System for DGGE, 220/240 V, for 16 cm gels with single prep well (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9082
DCode System for DGGE, 100 V, for 16 cm gels with single prep well (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9083
DCode System for DGGE, 120 V, for 10 cm gels with 2 prep wells (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9084
DCode System for DGGE, 220/240 V, for 10 cm gels with 2 prep wells (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9085
DCode System for DGGE, 100 V, for 10 cm gels with 2 prep wells (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9086
DCode System for CDGE, 120 V, for 16 cm gels with 20 wells (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
170-9089
DCode System for TTGE, 120 V, for 16 cm gels with 20 wells (1 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels, control reagents for TTGE
170-9090
DCode System for TTGE, 220/240 V
170-9091
DCode System for TTGE, 100 V
170-9092
DCode System for SSCP, 120 V, for 20 cm gels with 20 wells (0.75 mm), includes comb gasket, 2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels, cooling tank adaptor for use with external cooling bath, control reagents for SSCP
170-9093
DCode System for SSCP, 220/240 V
170-9094
DCode System for SSCP, 100 V
170-9102
Complete DCode System, 120 V, Mac, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP systems
170-9103
Complete DCode System, 220/240 V, Mac, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP, plates, combs and spacers to cast 1 mm and 0.75 mm thick 10, 16, and 20 cm gels as described for the DGGE, CDGE, TTGE, and SSCP systems
170-9104
Complete DCode System, 100 V, Mac, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP systems
170-9105
Complete DCode System, 120 V, PC, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP, plates, combs and spacers to cast 1 mm and 0.75 mm thick 10, 16 and 20 cm gels as described for the DGGE, CDGE, TTGE, and SSCP systems
170-9106
Complete DCode System, 220/240 V, PC, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP, plates, combs and spacers to cast 1 mm and 0.75 mm thick 10, 16, and 20 cm gels as described for the DGGE, CDGE, TTGE, and SSCP systems
170-9107
Complete DCode System, 100 V, PC, includes electrophoresis/temperature control module with standard and cooling tanks, sandwich core, Model 475 gradient former with all accessories required to cast gradient gels, WinMelt software, control reagents for DGGE, CDGE, TTGE and SSCP, plates, combs and spacers to cast 1 mm and 0.75 mm thick 10, 16, and 20 cm gels as described for the DGGE, CDGE, TTGE, and SSCP systems