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EZ-Link (tm) Activated Peroxidase Antibody Labeling Kit 介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

EZ-Link (tm) Activated Peroxidase Antibody Labeling Kit 現(xiàn)貨供應(yīng)

PIERCE	31497	EZ-Link (tm) Activated Peroxidase Antibody Labeling Kit	1 KIT
PIERCE	31503	Pierce Peroxidase Conjugate Stabilizer, 25 mL.	25 ML
PIERCE	31569	Pierce(R) Fluorescein Conjugated Goat Anti-Mouse IgG (H+L),	2 MG

EZ-Link (tm) Activated Peroxidase Antibody Labeling Kit 品牌介紹

美國Pierce公司(www.pierce.com)是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要產(chǎn)品包括：

1)化學(xué)發(fā)光體和比色底物

2)分析試劑

3)熒光標(biāo)記探針

4)酶聯(lián)免疫分析試劑盒等

EZ-Link (tm) Activated Peroxidase Antibody Labeling Kit 僅用于科研

The Thermo Scientific Pierce Activated Peroxidase and Antibody Labeling Kit is designed to prepare and purify HRP-antibody conjugates with any previously unmodified primary or secondary antibody.

Activated Peroxidase is HRP enzyme that has been modified with glutaraldehyde to convert surface amines to active aldehydes groups that will spontaneously conjugate with amines on an antibody. The result is a conjugate where both the enzyme activity and the antigen binding activity are preserved. The Pierce Activated Peroxidase Kit includes a Protein A/G column and buffers to affinity-purify the antibody conjugate from excess, unreacted enzyme.

Highlights:

Reacts with primary amines to form covalent amide bonds; no reduction step is necessary to secure the linkage
Activated enzyme can be stored for at least 12 months at -20°C
One milligram of activated enzyme is sufficient to produce about 0.5mL of conjugate with a working dilution of 1:1000 when coupled to a high-titer antibody
Enzyme activity is greater than 200 units/mg