MATERIALS REQUIRED BUT NOT SUPPLIED

1. Microplate reader with 450 ± 10nm filter.

2. Precision single or multi-channel pipettes and disposable tips.

3. Eppendorf Tubes for diluting samples.

4. Deionized or distilled water.

5. Absorbent paper for blotting the microtiter plate.

6. Container for Wash Solution

 STORAGE OF THE KITS 

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, DetectionReagent A, Detection Reagent B and the 96-well strip plate should be stored at -20oC upon receipt while theothers should be at 4oC.

2. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the abovestorage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and

reseal along entire edge of zip-seal.

Note:

It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.

SAMPLE PREPARATION 

1. Uscn, Inc. is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.

2. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.

3. If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.

4. Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts from certain chemicals.

5. Due to the possibility of mismatching between antigen from other origin and antibody used in our kits （e.g.,antibody targets conformational epitope rather than linear epitope）, some native or recombinant proteins from other manufacturers may not be recognized by our products.

6. Influenced by the factors including cell viability, cell number or sampling time, samples from cell culture supernatant may not be detected by the kit.

7. Fresh samples without long time storage is recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.

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