毛細(xì)管電泳應(yīng)用于測(cè)定牛血清白蛋白與脂質(zhì)體的相互作用
厲紅,屈鋒,徐建棟,鄧玉林
北京理工大學(xué)生命科學(xué)與技術(shù)學(xué)院, 北京 100081
收稿日期 2008-2-21  修回日期 2008-7-30  網(wǎng)絡(luò)版發(fā)布日期  2008-7-30  接受日期  2008-7-30 
摘要  建立了一種用毛細(xì)管電泳法檢測(cè)牛血清白蛋白（BSA）與脂質(zhì)體相互作用的分析方法。氧化指數(shù)的測(cè)定實(shí)驗(yàn)結(jié)果表明經(jīng)過冷凍干燥的脂質(zhì)體穩(wěn)定性更好;毛細(xì)管電泳表征脂質(zhì)體的電荷性質(zhì)實(shí)驗(yàn)結(jié)果表明脂質(zhì)體在pH 5.0～8.0的條件下呈電中性。在pH 7.0的條件下,以各種濃度的脂質(zhì)體混懸液為電泳緩沖液,以0.8%二甲亞砜（DMSO）為內(nèi)標(biāo),隨著緩沖液中脂質(zhì)體質(zhì)量濃度從0增加到2.4 mg/mL,BSA的有效淌度從-2.232×10-4 cm2·V－1·s－1變化到-3.046×10-4 cm2·V－1·s－1;結(jié)合Scatchard分析,測(cè)得BSA與脂質(zhì)體的結(jié)合常數(shù)為2.522×103（g/mL）－1。該方法簡(jiǎn)單、快速,為研究蛋白質(zhì)與脂質(zhì)體的相互作用提供了新的技術(shù)手段。
關(guān)鍵詞  毛細(xì)管電泳  牛血清白蛋白  脂質(zhì)體 


Investigation on interaction between bovine serum albumin and liposome using capillary electrophoresis
LI Hong, QU Feng, XU Jiandong, DENG Yulin
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China

 
  Abstract
  A method for the investigation on the interaction between bovine serum albumin （BSA） and liposome using capillary electrophoresis was developed. The oxidation index showed that the liposomes after freeze-drying were more stable. The results obtained from the capillary electrophoretic analysis of liposome showed that liposome had no charge at pH 5.0-8.0. A series of liposome suspension at different concentrations with the internal marker of 0.8% dimethyl sulfoxide （DMSO） were introduced as the electrophoresis buffer at pH 7.0. Along with the liposome concentrations raised from 0 to 2.4 mg/mL, it was found that the effective mobility of BSA changed from -2.232×10-4 cm2·V－1·s－1 to -3.046×10-4 cm2·V－1·s－1. The binding constant between BSA and liposome was 2.522×103（g/mL）-1 calculated by Scatchard analysis. This method is simple and rapid, and provides a new technology for the investigation on the interactions between protein and liposome. 
Key words  capillary electrophoresis （CE）  bovine serum albumin （BSA）  liposome