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作者： Leandro G. Nandi, João P.T.A. Guerra, Ismael C. Bellettini, Vanderlei G. Machado, Edson Minatti

Departamento de Química, Universidade Federal de Santa Catarina, UFSC, Florianópolis, SC 88040-900, Brazil

 

摘要：Morphological and conformational transitions of lentinan (LT), a β-glucan extracted from Shiitake mushrooms (Lentinula edodes), were investigated at different concentrations of aqueous NaOH, using Small Angle X-ray Scattering (SAXS) technique. At low NaOH(aq) concentration LT chains are self-associated and adopt the triple helix form where as at higher NaOH concentrations the polymer chains undergo a transition to random coil chains. Also, the presence of fractal dimensions was observed through analysis of the exponential decay of the scattering intensity as a function of the scattering angle. In addition, the lateral radius of gyration was determined for LT in different concentrations of NaOH solution, indicating a rigid triple helix present as a small rod-like structure. Interactions of LT with two zwitterionic surfactants were investigated by surface tension, fluorescence, and static light scattering measurements. Experimental data showed that the formation of LT–(surfactant) complexes occurred through a cooperative process.

 

關(guān)鍵詞：Polysaccharides; Helix-coil transition; Lentinan; SAXS; Zwitterionic surfactants