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文獻(xiàn)名： Luminescent carbon nanodots based aptasensors for rapid detection of kanamycin residue

 

作者： Xuexia Lin^a, Jianlong Su^a, Honggui Lin^b, Xiangying Sun^c, Bin Liu^c, Ranjith Kumar Kankala^a, Shu-Feng Zhou^a

^aDepartment of Chemical Engineering& Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, 361021, China

^bSchool of Marine Engineering, Jimei University, Xiamen, 361021, China

^cCollege of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, China

 

摘要：Despite the success in long-term storage of food and dietary products using antibiotics as supplements, enormous levels of their residues have remained as a significant health concern, leading to severe toxicity issues on consumption. Herein, we report an ultrasensitive and highly selective aptasensor based on carbon nanoparticles (CNPs) through a fluorescence-based aptamer-linked immunosorbent assay (FALIA) for rapid detection of kanamycin (KAA) residue. The fabricated CNP-aptasensor exhibited superior selectivity with exceptional photoluminescence properties. Under the optimal conditions, the linear equation of standard KAA solution was Y = −0.2279LogX+1.3648 (R = −0.9893) ranged from 10−4 to 10−7?ppb with excellent relative standard deviations (RSD) between 3.12 and 5.59 % (n?=?3). Moreover, the limit of detection (LOD) was lower than 5.0?×?10−8?ppb. Together, the excellent recovery and significant efficacy in the rapid detection of antibiotics at a low level in milk indicate that this fabricated CNP-aptasensor has a great potential in the establishment of an efficient antibiotic detector system in food and other nutraceutical industries.