性猛交XXXX乱大交派对,四虎影视WWW在线观看免费 ,137最大但人文艺术摄影,联系附近成熟妇女

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊(cè)

當(dāng)前位置:
美國(guó)布魯克海文儀器公司>技術(shù)文章>Benserazide, a dopadecarboxylase inhibitor, suppresses tumor growth by targeting hexokinase 2

技術(shù)文章

Benserazide, a dopadecarboxylase inhibitor, suppresses tumor growth by targeting hexokinase 2

閱讀:380          發(fā)布時(shí)間:2017-9-22
 作者 Wei Li, Mengzhu Zheng, Shuangping Wu, Suyu Gao, Mei Yang, Zhimei Li, Qiuxia Min, Weiguang Sun, Lixia Chen, Guangya Xiang and Hua Li

 

 

摘要:

Background

Hexokinase (HK) is the rate-limiting enzyme in the first reaction of glycolysis. And Hexokinase 2 (HK2) is most closely related to malignant tumor which expresses at higher level compared with normal cells. HK2 plays a pivotal role in tumor initiation and maintenance, which provides a new target for cancer therapy.

 

Methods

Structure-based virtual ligand screening was used in hit identification from ZINC Drug Database. Microscale thermophoresis assay was performed to evaluate the binding affinity. Enzyme inhibition, cytotoxicity, apoptosis, intracellular ATP level, mitochondrial membrane potential (MMP), glucose uptake and lactate production experiments were undertaken in SW480 cells to identify Benz as a HK2 inhibitor. Western blot was used to test protein expression. SW480 cells xenograft mouse models were used for in vivo study. Nano-particles of Benz were prepared to improve the antitumor efficacy and tumor targeting of Benz. HPLC was used to measure the concentration of free Benz in tumor tissues.

 

Results

Benserazide (Benz), was identified as a selective HK2 inhibitor, could specifically bind to HK2 and significantly inhibit HK2 enzymatic activity in vitro. In addition, Benz reduced glucose uptake, lactate production and intracellular ATP level, and could cause cell apoptosis and an increased loss of MMP as well. In vivo study indicated that intraperitoneal (ip) injection of Benz at 300 and 600 mg/Kg suppressed cancer growth in tumor-bearing mice and no toxicity shown. To further improve the antitumor efficacy and tumor targeting of Benz, nano-particles of Benz was prepared. Liposomal Benz at 100 and 200 mg/Kg performed potent inhibitory effects on tumor-bearing mice, showing reduced dose and better efficacy.

 

Conclusions

Our study provides a new direction for the development of Benz and its analogues as novel antitumor agents for cancer therapy.

收藏該商鋪

請(qǐng) 登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

對(duì)比框

產(chǎn)品對(duì)比 產(chǎn)品對(duì)比 聯(lián)系電話 二維碼 意見(jiàn)反饋 在線交流

掃一掃訪問(wèn)手機(jī)商鋪
010-62081908
在線留言
自贡市| 普格县| 通城县| 大丰市| 达日县| 绥棱县| 闽侯县| 武定县| 米泉市| 弥渡县| 类乌齐县| 洛浦县| 安平县| 静乐县| 二手房| 麻栗坡县| 天柱县| 东方市| 从化市| 博爱县| 呼和浩特市| 凉山| 平度市| 广西| 邳州市| 三都| 隆回县| 麦盖提县| 瑞昌市| 合水县| 清徐县| 肥东县| 清远市| 惠水县| 马关县| 怀柔区| 高碑店市| 滦南县| 房山区| 什邡市| 丰都县|