Technovit9100樹脂包埋試劑盒

Newly introduced! Technovit® 9100 is an embedding medium system based on methyl methacrylate （MMA）, which hardens at low temperature. It is designed for the embedding of mineralized tissues with extensive possibilities of staining for light microscopy.

Applications:

• Heavy duty microtome technique for preparing thin sections – such as pelvic biopsy, small low and high density bone samples.
• Cutting grinding and micro-grinding system （contact point technique） - such as jaw and teeth segments with and without implant.
• Combined contact point and heavy duty microtome technique （target preparation） - such as interface regions and examination of tissues surrounding metal implants and cement-free endoprothesis （target tissue preparation）.

Properties:

Technovit® 9100 Polymerization takes place under the exclusion of oxygen with the help of a peroxide/amine catalyst. Additional components such as PMMA-powder and a regulator allows for a controlled polymerization at temperatures between –8 and –20°C, which guarantees for the dispersion of the heat generated during polymerization. The polymerization time is between 18 and 24 hours using a volume between 3 and 15 ml in the above temperature range. All the routine techniques such as preparation tissues, staining, immuno- and enzyme-histochemistry, in-situ hybridization techniques can be performed.

• Basic Solution – is composed of organic monomers, with at least one carbon-carbon double bond （C=C）. The stabilizer added to this solution is for storage-stability. Hydrophilic properties are improved by the addition of a special hydrophilic generating agent.
• PMMA-Powder – is an internal filler and it is made up of PMMAmicro pellets. It is used to:
  - Reduce the polymerization shrinking effect.
  - Reduce the heat generated during the polymerization process. 
  - Improve the quality of the polymerization block.

• Hardener 1 – is one of the components of the polymerization initiation system. It is a derivative of dibenzoyl peroxide, which in combination with Hardener 2, makes the polymerization take place.
• Hardener 2 – it works catalytically upon hardener 1 to allows for a controlled polymerization, even at temperatures below 0°C.
• Regulator – is composed of a reactive organic compound, which allows a controlled polymerization, even with large volumes of polymer, without increases in the temperature during the polymerization reaction.
• PMMA-Granulate, EXART® - this granulate acts as an additional internal filler when larger amounts （500-1000 ml） of polymer are to be used, for example, in the case of femur shaft with noncemented endoprosthses. The amount of monomer （basic solution） is thereby reduced, at the same time making the polymerization easier to control.

Technovit9100樹脂包埋試劑盒

Technovit®9100聚合反應是在過氧化物/胺催化劑幫助下排除氧氣的情況下進行的。其他組分如PMMA-粉末和調節(jié)劑允許在-8和-20°C之間的溫度下進行受控聚合，這保證了聚合過程中產生的熱量的分散。在上述溫度范圍內，使用3至15ml體積的聚合時間為18至24小時。所有的常規(guī)技術，如準備組織，染色，免疫組化和酶組織化學，原位雜交技術都可以進行。
堿性溶液 - 由有機單體組成，至少有一個碳 - 碳雙鍵（C = C）。添加到該溶液中的穩(wěn)定劑用于儲存穩(wěn)定性。通過添加特殊的親水性發(fā)生劑來改善親水性。
PMMA-粉末 - 是一種內部填充物，由PMMAmicro顆粒組成。它用于：
- 減少聚合收縮效應。
- 減少聚合過程中產生的熱量。
- 改善聚合塊的質量。
硬化劑1 - 是聚合引發(fā)體系的組分之一。它是過氧化二苯甲酰的衍生物，與硬化劑2結合使用，可以發(fā)生聚合反應。
固化劑2 - 它在固化劑1上起催化作用，即使在低于0°C的溫度下也可進行受控聚合。
調節(jié)器 - 由反應性有機化合物組成，即使在大量聚合物的情況下也可以進行受控聚合反應，而不會在聚合反應過程中升高溫度。
PMMA-顆粒，EXART®--當需要使用大量（500-1000毫升）聚合物時，這種顆粒作為額外的內部填充劑，例如，對于非內源性endoprosthses股骨干的情況下。由此降低單體（堿性溶液）的量，同時使聚合更容易控制。