化工儀器網(wǎng)
可拉伸細(xì)胞基底硬度控制培養(yǎng)皿（CellSoft 100mm Round Dishes）
Cells sense soft! CellSoft offers softer substrates to match the material properties of tissue niches to better meet the needs of biological laboratories wanting to grow their cells on native stiffness。
直徑100mm培養(yǎng)皿，總生長(zhǎng)表面積為57cm2
●BioFlex® CellSoft標(biāo)準(zhǔn)6孔板
●腔室載玻片CellSoft
CellSoft培養(yǎng)板有很多不同的種類，如不同的硬度，不同的孔板，用于顯微觀察的腔室載玻片（圓形多孔板），共價(jià)包被CollagenI或其他蛋白，可對(duì)細(xì)胞進(jìn)行靜態(tài)或動(dòng)態(tài)牽拉應(yīng)力刺激。更重要的一點(diǎn)，新型的CellSoft培養(yǎng)板可以反復(fù)酶消化和再接種細(xì)胞，蛋白包被的表面可以重復(fù)使用多達(dá)三次。
niche。●彈性模量范圍1-80kPa
●BioFlex® CellSoft標(biāo)準(zhǔn)6孔板
●腔室載玻片CellSoft
Amino,
Elastin,
and Laminin (YIGSR)
and untreated （未處理）
納米圖案化牽張、壓縮培養(yǎng)表面提供細(xì)胞微環(huán)境，模仿天然細(xì)胞外基質(zhì)的對(duì)齊結(jié)構(gòu)，促進(jìn)細(xì)胞結(jié)構(gòu)和功能發(fā)展。
PUBLICATIONS
Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672Actin flows induce a universal coupling between cell speed and cell persistence
P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386Geometric friction directs cell migration
M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101Mitotic rounding alters cell geometry to ensure efficient spindle assembly
O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414Exploring the Function of Cell Shape and Size during Mitosis
C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169Methods for Two-Dimensional Cell Confinement
M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29
納米圖案化牽張、壓縮培養(yǎng)表面提供細(xì)胞微環(huán)境，模仿天然細(xì)胞外基質(zhì)的對(duì)齊結(jié)構(gòu)，促進(jìn)細(xì)胞結(jié)構(gòu)和功能發(fā)展。
References
[1] D. Huh, G.A. Hamilton, and D. E. Ingber, “From 3D cell culture to organs-on-chips," TrendsCell Biol., vol. 21, no. 12, pp. 745–754, 2011.
[2] M. Ravi, V.Paramesh, S. R. Kaviya, E. Anuradha, and F. D. Paul Solomon, “3D cell culturesystems: Advantages and applications," J. Cell. Physiol., vol. 230,no. 1, pp. 16–26, 2015.
[3] J. W.Haycock, 3D cell culture: a review of current approaches andtechniques., vol. 695. 2011.
[4] F.Pampaloni, E. G. Reynaud, and E. H. K. Stelzer, “The third dimension bridgesthe gap between cell culture and live tissue.," Nat. Rev. Mol. CellBiol., vol. 8, no. 10, pp. 839–845, 2007.
[5] J. Lee, M.J. Cuddihy, and N. A. Kotov, “Three-dimensional cell culture matrices: state ofthe art.," Tissue Eng Part B Rev, vol. 14, no. 1, pp. 61–86, 2008.
[6] M.Vinci et al., “Advances in establishment and analysis ofthree-dimensional tumor spheroid-based functional assays for target validationand drug evaluation," BMC Biol., vol. 10, no. 1, p. 29, 2012.
[7] B. A.Justice, N. A. Badr, and R. A. Felder, “3D cell culture opens new dimensions incell-based assays," Drug Discov. Today, vol. 14, no. 1–2, pp.102–107, 2009.
[8] I.Meyvantsson and D. J. Beebe, “Cell culture models in microfluidicsystems.," Annu. Rev. Anal. Chem., vol. 1, pp. 423–449, 2008.
[9] E. W. K.Young and D. J. Beebe, “Fundamentals of microfluidic cell culture in controlledmicroenvironments," Chem Soc Rev, vol. 39, no. 3, pp. 1036–1048,2010.
[10] D. J.Beebe, G. a Mensing, and G. M. Walker, “Physics and applications ofmicrofluidics in biology.," Annu. Rev. Biomed. Eng., vol. 4, pp.261–286, 2002.
[11] J. El-Ali,P. K. Sorger, and K. F. Jensen, “Cells on chips.," Nature, vol.442, no. 7101, pp. 403–411, 2006.
[12] J.Guck et al., “Optical deformability as an inherent cell marker fortesting malignant transformation and metastatic competence," Biophys J,vol. 88, no. 5, pp. 3689–3698, 2005.
[13] S.Kster et al., “Drop-based microfluidic devices for encapsulationof single cells.," Lab Chip, vol. 8, no. 7, pp. 1110–1115, 2008.
[14] H.Andersson and A. Van den Berg, “Microfluidic devices for cellomics: Areview," Sensors Actuators, B Chem., vol. 92, no. 3, pp. 315–325,2003.
[15] M. W.Tibbitt and K. S. Anseth, “Hydrogels as extracellular matrix mimics for 3D cellculture," Biotechnol. Bioeng., vol. 103, no. 4, pp. 655–663, 2009.
[16] J. P.Vacanti and R. Langer, “Tissue engineering: the design and fabrication ofliving replacement devices for surgical reconstruction andtransplantation.," Lancet, vol. 354, p. SI32-I34, 1999.
[17] G. S. D.Hetal Patel, Minal Bonde, “Biodegradable polymer scaffolds for tissueengineering," Trends Biomater. Artif. Organs, vol. 25, no. 1, pp.20–29, 2011.
[18] L. G.Griffith and M. A. Swartz, “Capturing complex 3D tissue physiology invitro.," Nat. Rev. Mol. cell Biol., vol. 7, no. 3, pp. 211–24,2006.
[19] D. J.Tobin, “Scaffolds for Tissue Engineering and 3D Cell Culture," MethodsMol. Biol., vol. 695, no. 2, pp. 213–227, 2011.
[20] J.Naranda et al., “Polyester type polyHIPE scaffolds with an interconnectedporous structure for cartilage regeneration," Sci. Rep., vol. 6,no. February, p. 28695, 2016.
[21] B.Dhandayuthapani, Y. Yoshida, T. Maekawa, and D. S. Kumar, “Polymeric scaffoldsin tissue engineering application: A review," Int. J. Polym. Sci.,vol. 2011, no. ii, 2011.
[22] F. J.O’Brien, “Biomaterials & scaffolds for tissue engineering," Mater.Today, vol. 14, no. 3, pp. 88–95, 2011.
[23] A. L.Paguirigan and D. J. Beebe, “Microfluidics meet cell biology: Bridging the gap byvalidation and application of microscale techniques for cell biologicalassays," BioEssays, vol. 30, no. 9, pp. 811–821, Sep. 2008.
[24] F.-Q. Nie,M. Yamada, J. Kobayashi, M. Yamato, A. Kikuchi, and T. Okano, “On-chip cellmigration assay using microfluidic channels.," Biomaterials, vol.28, no. 27, pp. 4017–4022, 2007.
[25] A. Valster,N. L. Tran, M. Nakada, M. E. Berens, A. Y. Chan, and M. Symons, “Cell migrationand invasion assays," Methods, vol. 37, no. 2, pp. 208–215, 2005.
[26] C. R.Justus, N. Leffler, M. Ruiz-Echevarria, and L. V Yang, “In vitro cell migrationand invasion assays.," J. Vis. Exp., vol. 752, no. 88, p. e51046,2014.
[27] N.Kramer et al., “In vitro cell migration and invasionassays.," Mutat Res, vol. 752, no. 1, pp. 10–24, 2013.
[28] J. W. Hong,V. Studer, G. Hang, W. F. Anderson, and S. R. Quake, “A nanoliter-scale nucleicacid processor with parallel architecture.," Nat. Biotechnol., vol.22, no. 4, pp. 435–439, 2004.
[29] J. Q.Boedicker, L. Li, T. R. Kline, and R. F. Ismagilov, “Detecting bacteria anddetermining their susceptibility to antibiotics by stochastic confinement innanoliter droplets using plug-based microfluidics.," Lab Chip, vol.8, no. 8, pp. 1265–1272, 2008.
[30] G.Velve-Casquillas, M. Le Berre, M. Piel, and P. T. Tran, “Microfluidic tools forcell biological research," Nano Today, vol. 5, no. 1. pp. 28–47,2010.
[31] C. R.Terenna et al., “Physical Mechanisms Redirecting Cell Polarity andCell Shape in Fission Yeast," Curr. Biol., vol. 18, no. 22, pp.1748–1753, . 2008.
[32] G.Faure-andré, “Regulation of Dendritic Cell Migration by CD74, the MHC ClassII–Associated Invariant Chain," Science (80-. )., vol. 1705, no.December, 2008.
[33] S. M.McFaul, B. K. Lin, and H. Ma, “Cell separation based on size and deformabilityusing microfluidic funnel ratchets," Lab Chip, vol. 12, no. 13, pp.2369–2376, 2012.
[34] S. C. Hur,N. K. Henderson-MacLennan, E. R. B. McCabe, and D. Di Carlo,“Deformability-based cell classification and enrichment using inertialmicrofluidics.," Lab Chip, vol. 11, no. 5, pp. 912–920, 2011.
[35] H. W. Hou,Q. S. Li, G. Y. H. Lee, A. P. Kumar, C. N. Ong, and C. T. Lim, “Deformabilitystudy of breast cancer cells using microfluidics," Biomed. Microdevices,vol. 11, no. 3, pp. 557–564, 2009.
我公司專注生物力學(xué)和生物打印等生物醫(yī)學(xué)工程科研服務(wù)-10年經(jīng)驗(yàn)支持,
點(diǎn)擊查更多科研工具-應(yīng)用盡有
化工儀器網(wǎng)