詳細(xì)介紹
。
產(chǎn)品介紹:
從瓊脂糖凝膠中回收純化特定的DNA片段是一種很常規(guī)的技術(shù),但是大多數(shù)的方法有的不能去除瓊脂糖(瓊脂糖對(duì)下游操作影響很大),要么會(huì)出現(xiàn)DNA被剪斷或者得率很低的情況。
E.Z.N.A® Gel Extraction Kit采用硅膠柱純化技術(shù),適合從各種類(lèi)型各種濃度的瓊脂糖凝膠中回收100bp-20kb的DNA片段,回收率可達(dá)到60-70%,可直接應(yīng)用于酶促連接反應(yīng)、PCR擴(kuò)增、酶切以及標(biāo)記等實(shí)驗(yàn)。此外,該試劑盒還可以用從于PCR產(chǎn)物,酶切產(chǎn)物中直接純化DNA。將目的DNA片段從瓊脂糖凝膠中切下,加入Binding Buffe水浴溶解凝膠,而后轉(zhuǎn)移至HiBind®離心柱上離心結(jié)合DNA,經(jīng)兩步簡(jiǎn)單洗滌,再用Elution Buffer洗脫DNA。
快速--可以在15分鐘內(nèi)完成純化工作
可靠--合適的緩沖液保證DNA純度和高回收率
安全--無(wú)須接觸有害的有機(jī)物抽提
高質(zhì)--純化的DNA適合于各種應(yīng)用
片段大--可回收大于10kb的DNA片段
Omega瓊脂糖凝膠回收試劑盒D2500-01
參數(shù)
應(yīng)用:酶切,連接,PCR等
樣品:各種類(lèi)型或濃度的瓊脂糖凝膠
用量:<400mg凝膠
時(shí)間:15-20min
結(jié)合能力:20ug
洗脫體積:15-30ul
Omega瓊脂糖凝膠回收試劑盒D2500-01
其他規(guī)格:
質(zhì)粒抽提試劑盒系列 | |
產(chǎn)品編號(hào) | 產(chǎn)品名稱(chēng) |
質(zhì)粒小量提取試劑盒 I 型 :從小于5ml培養(yǎng)過(guò)夜的細(xì)菌培養(yǎng)液中純化約35ug質(zhì)粒DNA | |
D6943-00 | Plasmid Mini Kit I(5) |
D6943-01 | Plasmid Mini Kit I(100) |
D6943-02 | Plasmid Mini Kit I(200) |
D6943-04 | Plasmid Mini Kit I(1000)-v Spin Column |
質(zhì)粒小量提取試劑盒 II型 :從小于15ml培養(yǎng)過(guò)夜的菌液提取50-70ug高純度質(zhì)粒DNA | |
D6945-00 | Plasmid Mini Kit II(5) |
D6945-01 | Plasmid Mini Kit II(50) |
D6945-02 | Plasmid Mini Kit II(200) |
質(zhì)粒中量提取試劑盒:從15-50ml細(xì)菌培養(yǎng)液中純化200-250ug高純度的質(zhì)粒DNA | |
D6904-00 | Plasmid Midi Kit(2) |
D6904-01 | Plasmid Midi Kit(10) |
D6904-03 | Plasmid Midi Kit(25) |
D6904-04 | Plasmid Midi Kit(100) |
質(zhì)粒大量提取試劑盒:從50-250ml細(xì)菌培養(yǎng)液中提取1.0mg質(zhì)粒DNA | |
D6922-00 | Plasmid Maxi Kit(2) |
D6922-01 | Plasmid Maxi Kit(5) |
D6922-02 | Plasmid Maxi Kit(20) |
快速過(guò)濾超大量質(zhì)粒提取試劑盒:從200-500ml菌中提取2.5mg質(zhì)粒 | |
D6929-01 | Fastfilter Plasmid Mega Kit(2) |
D6929-02 | Fastfilter Plasmid Mega Kit(5) |
D6929-03 | Fastfilter Plasmid Mega Kit(20) |
宏量質(zhì)粒提取試劑盒:從1000-2500ml菌中提取10mg質(zhì)粒 | |
D6920-01 | Plasmid Giga Kit(2) |
D6920-02 | Plasmid Giga Kit(5) |
D6920-03 | Plasmid Giga Kit(20) |
質(zhì)粒快速中量提取試劑盒:快速?gòu)?/span>25-50ml菌液中純化250ug的高純度質(zhì)粒DNA | |
D6905-01 | Fastfilter Plasmid Midi Kit(5) |
D6905-03 | Fastfilter Plasmid Midi Kit(25) |
D6905-04 | Fastfilter Plasmid Midi Kit(100) |
質(zhì)??焖俅罅刻崛≡噭┖校?/span>快速?gòu)?/span>25-50ml菌液中純化1.0mg的高純度質(zhì)粒DNA | |
D6924-01 | Fastfilter Plasmid Maxi Kit(5) |
D6924-03 | Fastfilter Plasmid Maxi Kit(25) |
D6924-04 | Fastfilter Plasmid Maxi Kit(100) |
酵母質(zhì)粒提取試劑盒:從1.5ml-5ml酵母培養(yǎng)基中提取1ug的質(zhì)粒DNA | |
D3376-00 | Yeast Plasmid Kit (5) |
D3376-01 | Yeast Plasmid Kit (50) |
D3376-02 | Yeast Plasmid Kit (200) |
96孔板快速過(guò)濾質(zhì)粒提取試劑盒:以快速地從過(guò)濾板與結(jié)合96個(gè)1-1.5ml配合操作提取2-20ug質(zhì)粒DNA | |
D1097-00 | E-Z 96 Fastfilter Plasmid Kit(1x96) |
D1097-01 | E-Z 96 Fastfilter Plasmid Kit(4x96) |
D1097-02 | E-Z 96 Fastfilter Plasmid Kit(20x96) |
96孔板簡(jiǎn)單經(jīng)濟(jì)型質(zhì)粒提取試劑盒:高通量地從96個(gè)1-1.5ml菌液中抽提質(zhì)粒DNA | |
D1095-00 | E-Z 96 SE Plasmid Kit(1x96) |
D1095-01 | E-Z 96 SE Plasmid Kit(4x96) |
D1095-02 | E-Z 96 SE Plasmid Kit(20x96) |
96孔板Swift質(zhì)粒提取試劑盒:快速、經(jīng)濟(jì)地高通量提取質(zhì)粒DNA | |
D1532-00 | swift plasmid kit(1*96) |
D1532-01 | swift plasmid kit(4*96) |
D1532-02 | swift plasmid kit(20*96) |
超快速質(zhì)粒小量提取試劑盒:7分鐘內(nèi)快速純化小量質(zhì)粒 | |
D6947-01 | X-Press Plasmid(5) |
D6947-02 | X-Press Plasmid(50) |
D6947-03 | X-Press Plasmid(200) |
超快速96孔質(zhì)粒小量提取試劑盒:96孔板形式快速質(zhì)粒提取 | |
D1047-01 | E-Z 96 X-press Plasmid Kit(1×96) |
D1047-02 | E-Z 96 X-press Plasmid Kit(4×96) |
D1047-03 | E-Z 96 X-press Plasmid Kit(20×96) |
質(zhì)粒高純(高性能)小量提取試劑盒:從富含核酸核酸的菌株中純化高純度的質(zhì)粒DNA | |
D7043-01 | HP Plasmid Mini Kit I(50) |
D7043-02 | HP Plasmid Mini Kit I(200) |
D7045-01 | HP Plasmid Mini Kit II(50) |
D7045-02 | HP Plasmid Mini Kit II(200) |
質(zhì)粒高純高性能中/大量提取試劑盒:從25-50ml,50-250ml細(xì)菌培養(yǎng)液中純化250ug、1.0mg高純度質(zhì)粒DNA | |
D7004-01 | HP Plasmid DNA Midi Kit (10) |
D7004-02 | HP Plasmid DNA Midi Kit (50) |
D7022-01 | HP Plasmid DNA Maxi Kit(5) |
D7022-02 | HP Plasmid DNA Maxi Kit(20) |
無(wú)內(nèi)毒素小量質(zhì)粒提取試劑盒 I/II:從培養(yǎng)過(guò)夜的菌液中提取30ug/70ug無(wú)內(nèi)毒素質(zhì)粒DNA | |
D6948-00 | Endo-free Plasmid Mini Kit I(5) |
D6948-01 | Endo-free Plasmid Mini Kit I(50) |
D6948-02 | Endo-free Plasmid Mini Kit I(200) |
D6950-00 | Endo-free Plamid Mini Kit II (5) |
D6950-01 | Endo-free Plasmid Mini Kit II(50) |
D6950-02 | Endo-free Plasmid Mini Kit II(200) |
無(wú)內(nèi)毒素中/大量提取試劑盒:從25-50ml或50-250ml細(xì)菌培養(yǎng)液中純化200-250ug或800-1000ug無(wú)內(nèi)毒素質(zhì)粒DNA | |
D6915-00 | Endo-free Plasmid Midi Kit(2) |
D6915-01 | Endo-free Plasmid Midi Kit(10) |
D6915-03 | Endo-free Plasmid Midi Kit(25) |
D6915-04 | Endo-free Plasmid Midi Kit(100) |
D6926-01 | Endo-free Plasmid Maxi Kit(6) |
D6926-03 | Endo-free Plasmid Maxi Kit(25) |
D6926-04 | Endo-free Plasmid Maxi Kit(100) |
D6948-00B | Endo-free Plasmid Mini Kit I(5) |
D6948-01B | Endo-free Plasmid Mini Kit I(50) |
D6948-02B | Endo-free Plasmid Mini Kit I(200) |
D6950-00B | Endo-free Plamid Mini Kit II (5) |
D6950-01B | Endo-free Plasmid Mini Kit II(50) |
D6950-02B | Endo-free Plasmid Mini Kit II(200) |
D6915-00B | Endo-free Plasmid Midi Kit(2) |
D6915-01B | Endo-free Plasmid Midi Kit(10) |
D6915-03B | Endo-free Plasmid Midi Kit(25) |
D6915-04B | Endo-free Plasmid Midi Kit(100) |
D6926-00B | Endo-free Plasmid Maxi Kit(2) |
D6926-01B | Endo-free Plasmid Maxi Kit(6) |
D6926-03B | Endo-free Plasmid Maxi Kit(25) |
D6926-04B | Endo-free Plasmid Maxi Kit(100) |
無(wú)內(nèi)毒素大量質(zhì)粒提取試劑盒:從200-500ml菌中提取2.5mg無(wú)內(nèi)毒質(zhì)粒質(zhì)粒 | |
D6228-00 | Endo-free Plasmid Mega Kit(2) |
D6228-01 | Endo-free Plasmid Mega Kit(5) |
D6228-02 | Endo-free Plasmid Mega Kit(20) |
無(wú)內(nèi)毒素宏量質(zhì)粒提取試劑盒:從1000-2500ml菌中提取10mg無(wú)內(nèi)毒質(zhì)粒質(zhì)粒 | |
D6234-01 | Endo-free Plasmid Giga Kit(2) |
D6234-02 | Endo-free Plasmid Giga Kit(5) |
D6234-03 | Endo-free Plasmid Giga Kit(20) |
96孔板無(wú)內(nèi)毒素質(zhì)粒提取試劑盒: | |
D1099-01 | (1*96) |
D1099-02 | (4*96) |
D1099-03 | (20*96) |
BAC/PAC大型質(zhì)粒提取試劑盒:從1-5ml培養(yǎng)過(guò)夜的菌液中提取30ug BAC/PAC/P1等大片段質(zhì)粒 | |
D2156-00 | (5) |
D2156-01 | (50) |
D2156-02 | (200) |
BAC/PAC 大型質(zhì)粒小提/大提試劑盒 | |
D2154-00 | (2) |
D2154-01 | (5) |
D2154-02 | (20) |
96孔BAC/PAC大型質(zhì)粒提取試劑盒:高通量純化BAC/PAC/P1等大型質(zhì)粒DNA | |
D1056-01 | (4x96) |
D1056-02 | (20x96) |
D1055-01 | (4x96) |
D1055-02 | (20x96) |
無(wú)內(nèi)毒素BAC/PAC質(zhì)粒提取試劑盒: | |
D2157-00 | (5) |
D2157-01 | (50) |
D2157-02 | (200) |
M-13單鏈DNA試劑盒:從1-5ml的噬菌體培養(yǎng)液中提取單鏈?zhǔn)删wDNA | |
D6900-01 | M13 DNA Kit (50) |
D6900-02 | M13 DNA Kit (200) |
D1900-01 | M13 DNA Kit(4x96) |
M-13單鏈DNA小提/大提/96孔板提取試劑盒 | |
D6908-00 | (2) |
D6908-01 | (5) |
D6908-02 | (20) |
96孔磁珠質(zhì)粒小量提取試劑盒:從1-1.5ml培養(yǎng)過(guò)夜的菌液中提取2-10ug的質(zhì)粒 | |
M1256-01 | (4x96) |
M1256-02 | (24x96) |
M1260-01 | (50) |
M1260-02 | 200) |
磁珠質(zhì)粒大量提取試劑盒:從100-250ml培養(yǎng)過(guò)夜菌液中提取1-2mg的質(zhì)粒 | |
M1257-01 | (5) |
M1257-02 | (20) |
磁珠質(zhì)粒超大量提取試劑盒:從1-2L培養(yǎng)過(guò)夜的菌液中提取2-5mg的質(zhì)粒 | |
M1259-01 | (5) |
M1259-02 | (20) |
磁珠無(wú)內(nèi)毒素小量提取試劑盒:從96*1-1.5ml培養(yǎng)過(guò)夜的菌液中提取2-20ug的無(wú)內(nèi)毒素質(zhì)粒 | |
M1258-01 | (4x96) |
M1258-02 | (24x96) |
96孔磁珠無(wú)內(nèi)毒素大量提取試劑盒:從200-250ml培養(yǎng)過(guò)夜的菌液中提取1-2mg無(wú)內(nèi)毒素質(zhì)粒 | |
M1252-00 | |
M1252-01 | |
M1252-02 | |
磁珠無(wú)內(nèi)毒素超大量提取試劑盒:從1-2l培養(yǎng)過(guò)夜的菌液中提取2-5mg無(wú)內(nèi)毒素質(zhì)粒 | |
M1253-00 | |
M1253-01 | |
M1253-02 |