巰基反應(yīng)性熒光染料Cy5.5馬來酰亞胺

Cyanine 5.5應(yīng)用非常廣泛，不僅可用于生物大分子的標(biāo)記，還被用作一些小分子的衍生化試劑。maleimide（馬來酰亞胺）常被用來標(biāo)記巰基,Cyanine5.5 Maleimide與其他標(biāo)記巰基的熒光試劑(如FITC，TRITC等)相比，它反應(yīng)選擇更高。Cyanine5.5 Maleimide是近紅外染料，除了用于標(biāo)記多肽，蛋白和寡核苷酸氨基外，也常被用于小動物活體成像。因?yàn)樗荛_了很多物質(zhì)都有吸收的可見光區(qū)，吸收的近紅外光在生物組織中的穿透深度較大，而激發(fā)的熒光受生物組織本底的影響較少，所以能夠在深層組織產(chǎn)生信號。尤其是在700～900 nm的范圍中，水和血紅蛋白的吸收都很少，近紅外光可以深入組織內(nèi)部多達(dá)15 cm。同時，這類染料還擁有紫外光區(qū)染料和同位素標(biāo)記無法具備的生物安全性。Cyanine 5.5 (cy 5.5）是常用的近紅外熒光染料。

cyanine 5.5 Maleimide需要共溶劑，但由于其疏水性比Cyanine7弱，應(yīng)用更為廣泛。陜西新研博美生物提供三乙胺鹽酸鹽（triethylammonium salts）形式的cyanine 5.5 Maleimide，較其他公司鉀鹽形式的cyanine 5.5 Maleimide更易容，在光學(xué)性質(zhì)，反應(yīng)性相同的情況下，擁有更好的溶解性。

cyanine 5.5 Maleimide（馬來酰亞胺）廣譜學(xué)性質(zhì)與Alexa Fluor 680 Maleimide和DyLight 680 Maleimide類似，可作為其替代物。

巰基反應(yīng)性熒光染料Cy5.5馬來酰亞胺

技術(shù)資料

1.Ex(nm)：678

2.Em(nm)：701

3.分子量：1153.20 

4.溶劑：DMSO

5.結(jié)構(gòu)式：

熒光染料分類：

Dye NHS esters

• BDP FL NHS ester

• BDP R6G NHS ester

• BDP TMR NHS ester

• BDP TR NHS ester

• Coumarin 343 X NHS ester

• Cyanine3 NHS ester

• Cyanine3.5 NHS ester

• Cyanine5 NHS ester

• Cyanine5.5 NHS ester

• Cyanine7 NHS ester

• Cyanine7.5 NHS ester

• FAM NHS ester, 6-isomer

• Pyrenebutyric acid NHS ester

• ROX NHS ester, pure 6- isomer

• Sulfo-Cyanine3 NHS ester

• Sulfo-Cyanine5 NHS ester

• Sulfo-Cyanine5 bis-NHS ester

• Sulfo-Cyanine5.5 NHS ester

• Sulfo-Cyanine7 NHS ester

Dye azides
BDP FL azide

• BDP TMR azide

• Coumarin 343 X azide

• Coumarin 343 azide

• Cyanine3 azide

• Cyanine3.5 azide

• Cyanine5 azide

• Cyanine5.5 azide

• Cyanine7 azide

• Cyanine7.5 azide

• FAM azide, 5-isomer

• FAM azide, 6-isomer

• PEP azide

• Perylene azide

• Pyrene azide 1

• Pyrene azide 2

• Pyrene azide 3

• R110 azide, 5- isomer

• R110 azide, 6- isomer

• ROX azide, 5- isomer

• Sulfo-Cyanine3 azide

• Sulfo-Cyanine5 azide

• Sulfo-Cyanine5.5 azide

• Sulfo-Cyanine7 azide

• TAMRA azide, 5-isomer

Dye alkynes

• 1-Ethynyl pyrene

• 3-Ethynyl perylene

• BDP FL alkyne

• BDP TMR alkyne

• Cyanine3 alkyne

• Cyanine5 alkyne

• Cyanine5.5 alkyne

• Cyanine7 alkyne

• Cyanine7.5 alkyne

• FAM alkyne, 5-isomer

• FAM alkyne, 6-isomer

• ROX alkyne, 5-isomer

• Sulfo-Cyanine3 alkyne

• Sulfo-Cyanine5 alkyne

• Sulfo-Cyanine5.5 alkyne

• Sulfo-Cyanine7 alkyne

• TAMRA alkyne, 5-isomer

Dye maleimides

• BDP FL maleimide

• BDP TMR maleimide

• Cyanine3 maleimide

• Cyanine5 maleimide

• Cyanine5.5 maleimide

• Cyanine7 maleimide

• Cyanine7.5 maleimide

• FAM maleimide, 6-isomer

• Pyrene maleimide

• Sulfo-Cyanine3 maleimide

• Sulfo-Cyanine5 maleimide

• Sulfo-Cyanine7 maleimide

Dye hydrazides

• BDP FL hydrazide

• Cyanine3 hydrazide

• Cyanine5 Boc-hydrazide

• Cyanine5 hydrazide

• Cyanine5.5 hydrazide

• Cyanine7 hydrazide

• Cyanine7.5 hydrazide

• Pyrene hydrazide

Carboxylic acids

• BDP FL carboxylic acid

• BDP R6G carboxylic acid

• BDP TMR carboxylic acid

• Coumarin 343 X carboxylic acid

• Cyanine3 carboxylic acid

• Cyanine3.5 carboxylic acid

• Cyanine5 carboxylic acid

• Cyanine5.5 carboxylic acid

• Cyanine7 carboxylic acid

• Cyanine7.5 carboxylic acid

• Pyrenebutyric acid

• Sulfo-Cyanine3 carboxylic acid

• Sulfo-Cyanine5 carboxylic acid

• Sulfo-Cyanine5.5 carboxylic acid

• Sulfo-Cyanine7 carboxylic acid

• Sulfo-Cyanine7.5 carboxylic acid