詳細(xì)介紹
細(xì)胞融合儀配件-貼壁細(xì)胞轉(zhuǎn)基因電極PP35-2P電極 是設(shè)計用于原位貼壁細(xì)胞的電穿孔或在除樣品杯以外的其他容器內(nèi)進(jìn)行較大量的電穿孔操作,使用電極在原位對貼壁細(xì)胞進(jìn)行電穿孔避免了需使用化學(xué)品消化細(xì)胞以及低穿孔效率,中斷了細(xì)胞周期及細(xì)胞間的等諸多問題.貼壁細(xì)胞轉(zhuǎn)基因電極PP35-2P電極 應(yīng)用于哺乳動物細(xì)胞轉(zhuǎn)染,基因治療,蛋白或藥物的傳遞,也可應(yīng)用于植物和酵母。
細(xì)胞融合儀配件-貼壁細(xì)胞轉(zhuǎn)基因電極是一種可復(fù)用的電極,適用于配裝于6孔板的每個單孔或一個35mm直徑的培養(yǎng)皿,由13個間隔2mm的鍍金電極組成,適用于BTX各型電穿孔儀。
Technical
Standard Capabilities
Voltage Range: 0 - 300V
Pulse Length Range: 1 - 35 msec in PBS
Volume Range: 0.5 - 3.0 ml
Physical Characteristics
Weight: 6 oz
Number of Electrodes: 13
Electrode Thickness: 0.5 mm
Electrode Material: Gold Plated.
Generator Compatibility ECM 830, 630, 600, 399, 395, T 820
Ordering
PP35-2P Petri Pulser PP35-2P
References
Marrero et al., Journal of Biological Chemistry, 270 (26):15734-15738 (1995)
Scheiffer et al., Journal of Biological Chemistry, 271 (17):10329-10333 (1996)
Marrero et al., Journal of Biological Chemistry, 272 (39):24684-24690 (1997)
BTX Protocols PR0165 and PR0330 (1998)
BTX Protocols PR0290, PR00286, PR0359, PR0360, PR0364 (1998)