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Dionex CarboPac PA1 糖和氨基酸色譜柱
對于各種基質(zhì)中單糖、雙糖和線性多糖的測定，使用耐用的 Thermo Scientific? Dionex? CarboPac? PA1 分析和保護柱。 這款的陰離子交換柱與脈沖電流檢測技術(shù) (PAD) 結(jié)合使用，可提供高分辨率的分離。 Dionex CarboPac PA1 樹脂的* Thermo Scientific? Dionex? MicroBead? 薄殼結(jié)構(gòu)，使其在所有緩沖鹽溶液中可保持 pH 0–14 的穩(wěn)定性。 薄殼的樹脂結(jié)構(gòu)提供出色的質(zhì)量傳遞優(yōu)秀的傳質(zhì)，從而實現(xiàn)快速的梯度再平衡。
描述
用于碳水化合物分離的耐用型柱
經(jīng)驗證適用于各種正式的食物分析方法
特別適合針對營養(yǎng)標簽進行的食物分析
非常適用于中性與酸性單糖的分離
靈敏度低于 Dionex CarboPac PA10 或 PA20 柱
提供微孔、標準孔和半制備規(guī)格
關(guān)于 Dionex CarboPac 柱系列
Dionex CarboPac 系列提供精選的高性能液相色譜 (HPLC) 柱，每一款均為不同類別化合物而優(yōu)化。 與能夠檢測碳水化合物而不產(chǎn)生衍生物的 PAD 配合使用，這些色譜柱支持簡單可靠的糖醇、單糖和雙糖、多糖和低聚糖分離技術(shù)。
Dionex CarboPac 柱提供以下成分的高分辨率分離：
膳食纖維中的糖蛋白單糖和復(fù)合碳水化合物，包括果聚糖、麥芽糊精和支鏈淀粉
單糖，例如從糖蛋白水解物和植物水解物得到的單糖
單糖和雙糖
用途和特點
AminoPac PA1柱用于氨基酸的快速分離。特別適用于分析酸性和酸不穩(wěn)定性氨基酸，以及用陽離子交換分離不*的氨基酸對。
Dionex CarboPac PA1 糖和氨基酸色譜柱
訂貨信息：
Dionex CarboPac PA1 糖和氨基酸分析柱
| 分析柱 | |
| CarboPac PA1 Analytical Column (2 x 250 mm) | 057178 |
| CarboPac PA1 Analytical Column (4 X 250 mm) | 035391 |
| CarboPac PA1 Semi-Preparative Column (9 x 250 mm) | 039686 |
| CarboPac PA1 Semi-Preparative Column (22 x 250 mm) | 0SP2866 |
| 保護柱 | |
| CarboPac PA1 Guard Column (2 x 50 mm) | 057179 |
| CarboPac PA1 Guard Column (4 x 50 mm) | 043096 |
| CarboPac PA1 Guard (9 x 50 mm) | 063501 |
Ideal Column for the Separation of Neutral and Acidic Monosaccharides
The CarboPac? PA1 is an anion-exchange column for the separation of mono-, disaccharides, and specific oligosaccharides using an isocratic eluent. Sialic acid analysis has also been demonstrated on this column.
General-purpose column for isocratic determination of monosaccharides and disaccharides
Detection by pulsed amperometric detection; no derivatization needed
Approved for use in a variety of official methods for the analysis of foods
The unique MicroBead? pellicular structure of the CarboPac PA1 resin gives it stability from pH 0–14 at all concentrations of buffer salts. The pellicular resin structure allows excellent mass transfer resulting in fast gradient reequilibration.
The CarboPac PA1 column is used with pulsed amperometric detection; no derivatization is required. Sensitivity on the PA1 is not as high as with the CarboPac PA20 or PA10 for monosaccharide analysis. Thus the CarboPac PA1 is well suited for the analysis of foods for nutritional labeling. The CarboPac PA1 has been approved for use in a number of official methods.
| CarboPac PA1 Column for Routine Carbohydrate Analysis Specifications | |
|---|---|
| Dimensions | CarboPac PA1 Analytical Column: 4 × 250 mm and 2 × 250 mm CarboPac PA1 Guard Column: 4 × 50 mm and 2 × 50 mm CarboPac PA1 Semipreparative Column: 22 × 250 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Temperature range | 4–55 °C Recommended operating temperature: ambient |
| Typical operating conditions | 1500 psi at 1.0 mL/min (analytical and guard columns) Recommended flow rate: 1.0 mL/min (for 4 × 250 mm) |
| Mobile phase compatibility | pH 0–14; up to 2% of common HPLC solvents. Acetate or hydroxide eluents only. |
| Resin composition | 10-μm-diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500-nm MicroBead quaternary ammonium functionalized latex (5% cross-linked). |
| Anion-exchange capacity | Approximately 100 μeq/column (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded-ferrule-style end fittings. All components are nonmetallic. |
