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MTD、巴比妥二合一檢測板

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主營品牌:美國NovaBios、美國Cortez、國產(chǎn)創(chuàng)侖等等。

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MTD、巴比妥二合一檢測板

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【公司名稱】 廣州健侖生物科技有限公司

【 市場部 】       楊永漢
【】 
【騰訊  】
【公司地址】 廣州市清華科技園健新基地番禺石樓鎮(zhèn)健啟路63號二期2幢101-103室

包 囊圓形或橢圓形,直徑自5µm至100µm,具有一層富 有彈性的堅韌囊壁。囊內(nèi)滋養(yǎng)體稱緩殖子(bradyzoite)可不斷 增殖,內(nèi)含數(shù)個至數(shù)百個蟲體,在一定條件下可破裂,緩殖子重 新進入新的細胞形成新的包囊,可長期在組織內(nèi)生存。腸絨毛上皮細胞內(nèi)發(fā)育增殖,成熟的裂殖體為長橢圓形,內(nèi)含4~ 29個裂殖子,以10~15個居多,呈扇狀排列,裂殖子形如新月狀 ,前尖后鈍,較滋養(yǎng)體為小。鑒于弓形蟲病原學檢查的不足和血 清學技術(shù)的進展,血清診斷已成為當今廣泛應用的診斷手段。方 法種類較多,主要有:1.染色試驗(dye test,DT)為經(jīng)典的特異血清學方法,采用活滋養(yǎng)體在有致活因子的參與下 與樣本內(nèi)特異性抗體作用,使蟲體表膜破壞不為著色劑美藍所染 。鏡檢見蟲體不被藍染者為陽性,蟲體多數(shù)被藍染者為性。2.間接血凝試驗(IHA)此法特異、靈敏、簡易,適用于流行病學調(diào)查及篩查性抗體檢測 ,應用廣泛。3.間接免疫熒光接體試驗(IFA)以整蟲為抗原,采用熒光標記的二抗檢測特異抗體。此法可測同 型及亞型抗體,其中測IgM適用與臨床早期診斷。4.酶聯(lián)免疫吸附試驗(ELISA)用于檢測宿主的特異循環(huán)抗體或抗原,已有多種改良法廣泛用于 早期急性感染和先天性弓形蟲病的診查?,F(xiàn)今將PCR及DNA探針技術(shù)應用于檢測弓形蟲感染,更具有靈敏、 特異、早期診斷的意義。當前也開始試用于臨床,限于實驗室條 件,國內(nèi)尚不能推廣應用。該病為動物源性疾病,分布于* 五大洲的各地區(qū),許多哺乳動物(約14種)、鳥類是本病的重要傳染源,人群感染也相當普遍。據(jù)血清 學調(diào)查,人群抗體陽性率為25%~50%。我國為5%~20%,多數(shù)屬隱 性感染。國內(nèi)人體弓形蟲病例,自從謝天華(1962)報告江西一 側(cè)先天性腦發(fā)育不全及脈絡膜視網(wǎng)膜炎的臨床病例以來,已有數(shù) 十個也分離出弓形蟲病原的病例。家畜的陽性率可達10%~50%, 常形成局部爆發(fā)流行,嚴重影響畜牧業(yè)發(fā)展,亦威脅人類健康。

The capsule is round or elliptical in shape, and its diameter ranges from 5 μm to 100 μm. It has a layer of resilient, tough capsules. The cyst trophozoite called bradyzoite can be continuously proliferated, containing several to several hundred worms. Under certain conditions, it can be broken. The bradyzoites can re-enter new cells to form new cysts. Survive within the organization. Intestinal villous epithelial cells develop and proliferate. Mature schizonts are oblong in shape and contain 4 to 29 merozoites. They are mostly 10 to 15 in fan-like arrangement. The merozoites resemble crescents. Back blunt, smaller than trophozoites. In view of the deficiencies in Toxoplasma gondii etiology and advances in serum technology, serodiagnosis has become a widely used diagnosis method today. There are many types of methods, mainly: 1. The dye test (DT test) is a classic, specific serological method using live trophozoites with the participation of specific agents in the sample under the influence of activating factors, so that the destruction of the worm's dermis is not caused by the colorant methylene blue. Microscopic examination showed that the worm body was not positive for blue dyeing, and most of the worm body was sexually blue. 2. Indirect hemagglutination test (IHA) This method is specific, sensitive, and simple. It is suitable for epidemiological investigations and screening antibody tests. It is widely used. 3. The indirect immunofluorescence assay (IFA) uses whole-worms as antigens, and fluorescently labeled secondary antibodies detect specific antibodies. This method can be used to measure isotype and subtype antibodies, of which IgM is applicable and early clinical diagnosis. 4. Enzyme-linked immunosorbent assays (ELISAs) are used to detect host-specific circulating antibodies or antigens. A variety of improved methods have been widely used for early acute infection and congenital toxoplasmosis. PCR and DNA probe technology are now used to detect Toxoplasma gondii infection, which is more sensitive, specific and early diagnostic. At present, it has also begun to be used in clinical trials. It is limited to laboratory conditions and cannot be applied in the country. The disease is an animal-borne disease and it is distributed in all regions of the world on five continents. Many mammals (about 14 species) and birds are important sources of infection of the disease. Population infection is also quite common. According to serological surveys, the population antibody positive rate was 25% to 50%. Our country is 5% to 20%, and most of them are latent infections. In domestic cases of Toxoplasma gondii in humans, since Tse Tianhua (1962) reported a clinical case of congenital cerebral hypoplasia and chorioretinitis in Jiangxi province, there have been ten cases of Toxoplasma gondii isolates. The positive rate of livestock can reach 10% to 50%, and it often forms a local outbreak, which seriously affects the development of animal husbandry and also threatens human health.

 

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