流感熒光定量RT-PCR診斷試劑盒

廣州健侖生物科技有限公司

廣州健侖長期供應(yīng)各種PCR試劑盒，主要代理進(jìn)口和國產(chǎn)品牌的流行病毒PCR檢測試劑盒。例如：甲乙型流感病毒核酸檢測試劑盒、黃熱病毒核酸檢測試劑盒、諾如病毒核酸檢測試劑盒、登革病毒核酸檢測試劑盒、基孔肯雅病毒核酸檢測試劑盒、結(jié)核桿菌核酸病毒檢測試劑盒、孢疹病毒核算檢測試劑盒、西尼羅河病毒PCR檢測試劑盒、呼吸道合胞病毒核酸檢測試劑盒、冠狀病毒PCR檢測試劑盒等等。蟲媒體染病系列、呼吸道病原體系列、發(fā)熱伴出疹系列、消化道及食源感染系列。

廣州健侖長期供應(yīng)各種流感檢測試劑，包括進(jìn)口和國產(chǎn)的品牌，主要包括日本富士瑞必歐、日本生研、美國BD、美國NovaBios、美國binaxNOW、英國clearview、凱必利、廣州創(chuàng)侖等主流品牌。

主要檢測：甲型流感病毒檢測試劑、乙型流感病毒檢測試劑、甲乙型流感病毒檢測試劑、A+B流感病毒檢測試劑盒、流感病毒抗原快速檢測卡、流感病毒抗體快速檢測試劑盒、流感快速檢測試劑 c1c2。

流感熒光定量RT-PCR診斷試劑盒

實時熒光定量PCR （Quantitative Real-time PCR）是一種在DNA擴(kuò)增反應(yīng)中，以熒光化學(xué)物質(zhì)測每次聚合酶鏈?zhǔn)椒磻?yīng)（PCR）循環(huán)后產(chǎn)物總量的方法。通過內(nèi)參或者外參法對待測樣品中的特定DNA序列進(jìn)行定量分析的方法。·
Real-timePCR是在PCR擴(kuò)增過程中，通過熒光信號，對PCR進(jìn)程進(jìn)行實時檢測。由于在PCR擴(kuò)增的指數(shù)時期，模板的Ct值和該模板的起始拷貝數(shù)存在線性關(guān)系，所以成為定量的依據(jù)。
所謂實時熒光定量PCR技術(shù)[1]  ，是指在PCR反應(yīng)體系中加入熒光基團(tuán)，利用熒光信號積累實時監(jiān)測整個PCR進(jìn)程，zui后通過標(biāo)準(zhǔn)曲線對未知模板進(jìn)行定量分析的方法。
檢測方法
1.SYBRGreenⅠ法：
在PCR反應(yīng)體系中，加入過量SYBR熒光染料，SYBR熒光染料特異性地?fù)饺隓NA雙鏈后，發(fā)射熒光信號，而不摻入鏈中的SYBR染料分子不會發(fā)射任何熒光信號，從而保證熒光信號的增加與PCR產(chǎn)物的增加*同步。
SYBR定量PCR擴(kuò)增熒光曲線圖
PCR產(chǎn)物熔解曲線圖（單一峰圖表明PCR擴(kuò)增產(chǎn)物的單一性）
2.TaqMan探針法：
探針完整時，報告基團(tuán)發(fā)射的熒光信號被淬滅基團(tuán)吸收；PCR擴(kuò)增時，Taq酶的5’-3’外切酶活性將探針酶切降解，使報告熒光基團(tuán)和淬滅熒光基團(tuán)分離，從而熒光監(jiān)測系統(tǒng)可接收到熒光信號，即每擴(kuò)增一條DNA鏈，就有一個熒光分子形成，實現(xiàn)了熒光信號的累積與PCR產(chǎn)物的形成*同步。
1. TaqMan熒光探針：PCR擴(kuò)增時在加入一對引物的同時加入一個特異性的熒光探針，該探針為一寡核苷酸，兩端分別標(biāo)記一個報告熒光基團(tuán)和一個淬滅熒光基團(tuán)。探針完整時，報告基團(tuán)發(fā)射的熒光信號被淬滅基團(tuán)吸收；PCR擴(kuò)增時，Taq酶的5'－3'外切酶活性將探針酶切降解，使報告熒光基團(tuán)和淬滅熒光基團(tuán)分離，從而熒光監(jiān)測系統(tǒng)可接收到熒光信號，即每擴(kuò)增一條DNA鏈，就有一個熒光分子形成，實現(xiàn)了熒光信號的累積與PCR產(chǎn)物形成*同步。而新型TaqMan-MGB探針使該技術(shù)既可進(jìn)行基因定量分析，又可分析基因突變（SNP），有望成為基因診斷和個體化用藥分析的*技術(shù)平臺。
2. SYBR熒光染料：在PCR反應(yīng)體系中，加入過量SYBR熒光染料，SYBR熒光染料非特異性地?fù)饺隓NA雙鏈后，發(fā)射熒光信號，而不摻入鏈中的SYBR染料分子不會發(fā)射任何熒光信號，從而保證熒光信號的增加與PCR產(chǎn)物的增加*同步。SYBR僅與雙鏈DNA進(jìn)行結(jié)合，因此可以通過溶解曲線，確定PCR反應(yīng)是否特異。
3. 分子信標(biāo)：是一種在5和3末端自身形成一個8個堿基左右的發(fā)夾結(jié)構(gòu)的莖環(huán)雙標(biāo)記寡核苷酸探針，兩端的核酸序列互補(bǔ)配對，導(dǎo)致熒光基團(tuán)與淬滅基團(tuán)緊緊靠近，不會產(chǎn)生熒光。PCR產(chǎn)物生成后，退火過程中，分子信標(biāo)中間部分與特定DNA序列配對，熒光基因與淬滅基因分離產(chǎn)生熒光。
 

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】     歐

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【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

Quantitative real-time PCR (Quantitative Real-time PCR) is a method of measuring the total amount of product after each PCR reaction with a fluorescent chemical in a DNA amplification reaction. A method for quantitative analysis of a specific DNA sequence in a test sample by internal or external parameters. ·
Real-time PCR is a real-time PCR process that uses fluorescence signals during PCR amplification. Since there is a linear relationship between the Ct value of the template and the initial copy number of the template during the PCR amplification exponential phase, it becomes a quantitative basis.
The so-called real-time fluorescence quantitative PCR technology [1], refers to the PCR reaction system by adding fluorescent groups, the use of fluorescence signal accumulation in real-time monitoring of the entire PCR process, and finally through the standard curve of quantitative analysis of unknown template method.
Detection method
1.SYBR Green I method:
In the PCR reaction system, an excess of SYBR fluorescent dye is added, and the SYBR fluorescent dye is specifically doped into the DNA double-stranded and emits a fluorescent signal, while the SYBR dye molecules not incorporated into the chain do not emit any fluorescence signal, thereby ensuring the fluorescence signal The increase is compley synchronized with the increase in PCR product.
SYBR quantitative PCR amplification fluorescence curve
PCR product melting curve (single peak graph shows the PCR amplification product of the unity)
2. TaqMan probe method:
When the probe is intact, the fluorescent signal emitted by the reporter group is absorbed by the quencher group. During the PCR amplification, the 5'-3 'exonuclease activity of the Taq enzyme degrades the probe so that the fluorescent group and the quencher Eliminate the fluorescence group separation, so fluorescence monitoring system can receive the fluorescence signal, that is, for each amplification of a DNA chain, there is a fluorescent molecule formation, the accumulation of fluorescence signals and the formation of PCR products compley synchronized.
1. TaqMan Fluorescent Probe: A pair of primers is added to a specific fluorescent probe during PCR amplification. The probe is an oligonucleotide, labeled with a reporter fluorescent group and a quencher Fluorescent groups. When the probe is intact, the fluorescent signal emitted by the reporter group is absorbed by the quencher group. During the PCR amplification, the 5'-3 'exonuclease activity of the Taq enzyme degrades the probe so that the fluorescent group and the quencher Eliminate the fluorescence group separation, so fluorescence monitoring system can receive the fluorescence signal, that is, for each amplification of a DNA chain, there is a fluorescent molecule formation, the accumulation of fluorescence signal and the PCR product is compley synchronized. The new TaqMan-MGB probe enables both quantitative gene analysis and genetic mutation (SNP) analysis and is expected to be the technology of choice for gene diagnosis and individualized drug use analysis.
2. SYBR Fluorescent dye: SYBR fluorescent dye is added to PCR reaction system, SYBR fluorescent dye non-specific incorporation of DNA double-stranded, fluorescence signal is emitted, but not incorporated into the chain of SYBR dye molecules will not emit any fluorescence Signal, thereby ensuring that the increase of the fluorescence signal is compley synchronized with the increase of the PCR product. SYBR binds only to double-stranded DNA, so you can determine if the PCR reaction is specific by lysing the curve.
3. Molecular Beacons: A stem-ring double-labeled oligonucleotide probe that forms a hairpin structure of about 8 bases by itself at the ends 5 and 3, and the nucleic acid sequences at both ends are complementary-matched to each other, resulting in the interaction of the fluorophore with Quenched groups tightly close, will not produce fluorescence. After the PCR product is generated, during annealing, the middle part of the molecular beacon is paired with the specific DNA sequence, and the fluorescent gene is separated from the quencher gene to generate fluorescence