IgA(免疫球蛋白A)

廣州健侖生物科技有限公司

IgA可作為免疫球蛋白IgA的α 鏈參考依據(jù)，可用于淋巴瘤、漿細(xì)胞瘤和B細(xì)胞來源的何杰金氏淋巴瘤的研究。也可用于腎小球腎炎的功能性分類和一些免疫性疾病的研究。

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IgA(免疫球蛋白A)

【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞漿

適用組織：石蠟/冰凍

陽性對(duì)照：扁桃體

抗原修復(fù)：熱修復(fù)（檸檬酸）

抗體孵育時(shí)間：30-60min

IgA(免疫球蛋白A)

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】     歐

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

首先作者以普通大鼠腎臟細(xì)胞為研究對(duì)象，發(fā)現(xiàn)這些細(xì)胞在遷移的過程中會(huì)不斷地向胞外釋放內(nèi)容物，這種新發(fā)現(xiàn)的細(xì)胞事件被稱為遷移胞吐作用(migracytosis)。細(xì)胞在遷移的過程中會(huì)將其收縮纖維留在胞體后側(cè)。在收縮纖維的橫截面處會(huì)有很多直徑約為3um的囊泡，即為新發(fā)現(xiàn)的"遷移小體"。這些遷移小體中還包括有很多直徑約50~100nm的小泡。zui終收縮纖維會(huì)發(fā)生斷裂，從而這些遷移小體會(huì)釋放到胞外并被周圍的細(xì)胞吞噬。
由于之前沒有任何相關(guān)的報(bào)道，為了進(jìn)一步研究，需要進(jìn)行純化。作者通過密度梯度離心的方法得到了細(xì)胞裂解物的不同組分，比較發(fā)現(xiàn)migrasome富集在某一特定的組分中。之后，作者通過質(zhì)譜的手段篩選了一系列migrasome特異性的蛋白，在大量膜蛋白與細(xì)胞骨架相關(guān)蛋白的候選物中，作者發(fā)現(xiàn)Tetraspanin-4(TSPAN4)是其中migrasome定位zui準(zhǔn)確的蛋白質(zhì)，因此作者人工構(gòu)建了(TSPAN4)-熒光報(bào)告基因供后續(xù)研究。進(jìn)一步研究發(fā)現(xiàn)：當(dāng)細(xì)胞密度很大時(shí)，TSPAN4在細(xì)胞內(nèi)的分布十分彌散，只有在細(xì)胞膜上有少量富集;但是當(dāng)細(xì)胞密度較低時(shí)，隨著細(xì)胞收縮纖維的伸長(zhǎng)，很多TSPAN4富集于收縮纖維區(qū)域，特別是其表面的環(huán)狀結(jié)構(gòu)。之后，作者通過比較共聚焦顯微鏡成像結(jié)果與電鏡投射結(jié)果，證實(shí)了這部分"環(huán)狀"的結(jié)構(gòu)即之前發(fā)現(xiàn)的migrasome。

First of all, the author used ordinary rat kidney cells as the research object and found that these cells continuously release the contents to the outside during the process of migration. The newly discovered cell event is called migracytosis. Cells migrate their contractile fibers to the back of the cell body during migration. There are many vesicles about 3um in diameter at the cross-section of the deflated fibers, the newly discovered "migrating bodies." These migration bodies also include many small bubbles about 50 ~ 100nm in diameter. The final defibrillated fibers break and the migrating bodies release to the extracellular and are swallowed by the surrounding cells.
Since there was no previous report, for further study, purification was required. The authors obtained different components of cell lysates by density gradient centrifugation, and found that migrasome is enriched in a particular component. After that, the authors screened a series of migrasome-specific proteins by mass spectrometry. In a large number of candidates for membrane proteins and cytoskeleton-associated proteins, the authors found that Tetraspanin-4 (TSPAN4) is the most accurate protein in which migrasome is located, The (TSPAN4) -fluorescent reporter gene was constructed manually for later study. Further studies showed that when the cell density is very high, the distribution of TSPAN4 in the cell is very diffuse, and only a small amount of enrichment is found in the cell membrane. However, when the cell density is low, many TSPAN4 are enriched with the cell shrinkage fiber elongation In the area of ??constricted fibers, especially the surface of the ring structure. Afterwards, the authors confirmed the "ring-like" structure of migrasome, which was previously found by comparing confocal microscopy imaging with electron microscopic projection results.