CD8(T細(xì)胞)兔單克隆抗體

廣州健侖生物科技有限公司

CD8作為T細(xì)胞受體的共受體，是一種跨膜糖蛋白。在細(xì)胞毒性T淋巴細(xì)胞/抑制T淋巴細(xì)胞呈現(xiàn)高表達(dá)，而NK細(xì)胞呈現(xiàn)低表達(dá)。在成熟T細(xì)胞中CD4和CD8常出現(xiàn)互相排斥的表達(dá)。所以，CD8常與CD4合用用于研究輔助性T淋巴細(xì)胞及其腫瘤，如外周T細(xì)胞淋巴瘤（CD4+/CD8-）；間變性大細(xì)胞淋巴瘤（CD4+/CD8-)。T淋巴母細(xì)胞淋巴瘤CD4和CD8可同時(shí)表達(dá)。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

歡迎咨詢

歡迎咨詢

【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞膜

克隆號(hào)：SP16

同型：IgG1

適用組織：石蠟/冰凍

陽性對(duì)照：扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

CD8(T細(xì)胞)兔單克隆抗體

2. 細(xì)胞毒試驗(yàn)
檢測(cè)CTL細(xì)胞毒作用均可采用檢測(cè)NK細(xì)胞殺傷活性的方法，僅效靶細(xì)胞比例不一樣，現(xiàn)將LDH釋放法簡(jiǎn)要敘述如下：
①  靶細(xì)胞為用作刺激細(xì)胞的自身或同種異體EBV-LCL細(xì)胞，調(diào)成1×105/ml；
②  效應(yīng)細(xì)胞為上述誘導(dǎo)的特異性CTL，調(diào)成2.5×106/ml；
③  各取0.1ml于96孔板中（效/靶比值為25:1）；輕輕吹打，使二者混勻；同時(shí)設(shè)靶細(xì)胞自然釋放對(duì)照組（即只加靶細(xì)胞而不加效應(yīng)細(xì)胞）和zui大釋放對(duì)照組（0.1ml靶細(xì)胞和0.1ml 1% NP-40）；
④  1000rpm/min離心2min后，置37°C、5% CO2 培養(yǎng)箱中孵育4hr
⑤  酶促顯色反應(yīng)：參見“NK細(xì)胞殺傷實(shí)驗(yàn)”。
中性粒細(xì)胞（polymorphonuclear leukocyte，PMN）的功能包括粘附、移動(dòng)、吞噬殺菌等，是機(jī)體天然免疫力的重要組成部分。
試劑及材料
1. 白色葡萄球菌
2. 肉湯培養(yǎng)基
3. 鹼性美藍(lán)液
操作方法
1. 菌液的制備  將白色葡萄球菌接種于中，放37℃溫箱內(nèi)培養(yǎng)12h左右；置水浴中加熱100℃ 10min殺死細(xì)菌，用無菌生理鹽水稀釋成6×108 細(xì)菌/ml備用；
2. 用血紅蛋白吸管吸取受試者耳垂或指血40μl，立即加入盛有20μl肝素（濃度為20U/ml）的潔凈凹玻片的凹孔內(nèi)，輕輕攪動(dòng)混勻， 再加上述葡萄球菌菌液20μl充分混勻。然后置入鋪有濕紗布的有蓋容器內(nèi)（此容器先放37℃溫箱中預(yù)溫），在37℃溫箱中作用30min，其間每隔10min搖勻一次；
3. 作用完畢，取1小滴混合液置于潔凈無油污的載玻片一端，推成薄片。待干后，用甲醇固定4～5min，鹼性美藍(lán)液染2～3min，置油鏡下觀察。隨機(jī)計(jì)數(shù)100個(gè)中性粒細(xì)胞，分別記錄發(fā)生吞噬和未吞噬的白細(xì)胞數(shù)，對(duì)有吞噬作用的白細(xì)胞，應(yīng)同時(shí)記錄所吞噬的細(xì)菌數(shù)；
結(jié)果分析
吞噬細(xì)胞%=即100個(gè)中性粒細(xì)胞中吞噬有細(xì)菌的細(xì)胞數(shù)；
吞噬指數(shù)=將100個(gè)中性粒細(xì)胞所吞噬的細(xì)菌總數(shù)除以100，得到每個(gè)白細(xì)胞吞噬細(xì)菌的平均數(shù)，即為吞噬指數(shù)。

CD8(T細(xì)胞)兔單克隆抗體

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

想了解更多的產(chǎn)品及服務(wù)請(qǐng)掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】     歐

【】 
【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

2. Cytotoxicity test
Detection of CTL cytotoxicity can be used to detect NK cell killing activity, only the proportion of target cells is not the same, now LDH release method is briefly described as follows:
① target cells used as stimulating cells or allogenic EBV-LCL cells, adjusted to 1 × 105 / ml;
② effector cells induced by the above-mentioned specific CTL, transferred to 2.5 × 106 / ml;
(3) Take 0.1ml each in a 96-well plate (effect / target ratio of 25: 1); gently blow to mix the two; at the same time set the target cells to release the control group ) And maximal release control (0.1 ml target cells and 0.1 ml 1% NP-40);
④ After centrifugation at 1000rpm / min for 2min, incubate for 4hr at 37 ° C in a 5% CO2 incubator
⑤ enzymatic color reaction: see "NK cell killing experiment."
The function of polymorphonuclear leukocyte (PMN), including adhesion, migration, phagocytosis and so on, is an important part of the body's natural immunity.
Reagents and materials
Staphylococcus aureus
2. Broth culture medium
3 alkaline blue liquid
Method of operation
1. Bacterial liquid preparation Staphylococcus aureus inoculated in, put 37 ℃ incubator for about 12h; water bath heated to 100 ℃ 10min to kill bacteria, diluted with sterile saline into 6 × 108 bacteria / ml spare;
2. Hemoglobin pipette to absorb the subject's earlobe or finger blood 40μl, immediay added 20μl heparin (concentration of 20U / ml) clean concave glass concave wells, gently agitated and mix, coupled with the Staphylococcus aureus Mix well 20μl. Then placed in a covered container wet gauze (the container first pre-37 ° temperature incubator), 37 ° C incubator role in 30min, during which shake once every 10min;
3. The effect is completed, take a small drop of liquid mixture placed in clean oil-free one end of the slide, pushed into thin slices. To be dry, fixed with methanol 4 ~ 5min, alkaline methylene blue dye stained 2 ~ 3min, set the oil microscope observation. Randomly counting 100 neutrophils were recorded phagocytic and non-phagocytic leukocytes were phagocytosis of white blood cells, should also record the number of bacteria phagocytosed;
Result analysis
% Of phagocytes = number of cells engulfed by bacteria in 100 neutrophils;
Phagocytosis Index = Divide the total number of bacteria phagocytosed by 100 neutrophils by 100 to get the average number of phagocytic bacteria per leukocyte, which is the phagocytic index.