CD57自然殺傷細(xì)胞（鼠單克隆抗體）

廣州健侖生物科技有限公司

CD57為自然殺傷細(xì)胞(Natural Killer Cell，亦稱NK細(xì)胞或Leu-7)，主要標(biāo)記淋巴組織中的NK細(xì)胞。此抗體可以檢測生正常NK細(xì)胞表面的CD57抗原，也可以識(shí)別T細(xì)胞某些亞類，在成人外周血單個(gè)核細(xì)胞約20%細(xì)胞呈陽性反應(yīng)?？捎糜贜K細(xì)胞介導(dǎo)的細(xì)胞毒、NK細(xì)胞及T細(xì)胞亞群的功能等方面的研究，亦可作為結(jié)節(jié)性淋巴細(xì)胞為主的霍奇金淋巴瘤和神經(jīng)內(nèi)分泌細(xì)胞及其腫瘤的參考依據(jù)。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

歡迎咨詢

歡迎咨詢

【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞膜

克隆號(hào)：NK-1

同型：IgM/K

適用組織：石蠟/冰凍

陽性對照：扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

CD57自然殺傷細(xì)胞（鼠單克隆抗體）

原理：
蘇丹紅染料在脂類物質(zhì)中的溶解度大于其在溶劑中的溶解度，如果染料與含有脂類的試樣接觸，便有大量染料進(jìn)入脂類物質(zhì)的結(jié)構(gòu)內(nèi)，使這些結(jié)構(gòu)呈紅色。多用蘇丹紅染料、油紅O、尼羅藍(lán)等溶于脂類的染料染色，使脂質(zhì)呈色。也可用抗原抗體染色，脂肪酸或膽堿可使抗原抗體還原為二氧化鋨而呈黑色。
配制試劑：
1.蘇丹紅Ⅲ染色液：將1g蘇丹紅Ⅲ溶于加溫的70%乙醇溶液中過濾備用；
2.甲醛鈣固定液：將10ml甲醛和1g CaCl2混合加水至100ml；
3.甘油明膠：將12g明膠加入120ml蒸餾水中水浴加熱使其*溶解，再加入60ml甘油和1g苯酚，充分混勻后即得。于4℃儲(chǔ)存?zhèn)溆茫?br />染色步驟：
1.用Hanks液漂洗蓋玻片3次；
2.用甲醛鈣固定液固定20min；
3.用蒸餾水漂洗蓋玻片3次；
4.用70%乙醇將蓋玻片沖洗3次；
5.用蘇丹紅Ⅲ染色液覆蓋蓋片樣品，染色10—30min；
6.用70%乙醇漂洗蓋片3次；
7.用甘油明膠封片后觀察；
結(jié)果：
原代細(xì)胞含脂類的區(qū)域呈橘紅色；
精原細(xì)胞的生理生化特性及其分裂增殖、分化、運(yùn)動(dòng)、衰老、死亡等項(xiàng)生命活動(dòng)的調(diào)節(jié)機(jī)制的深入研究，抗原抗體發(fā)生機(jī)理的進(jìn)一步闡明以及精原細(xì)胞異體、異種抗原抗體技術(shù)的實(shí)際應(yīng)用，都迫切需要找到一種可行的方法將其分離純化，以期得到較高產(chǎn)量和純度的有活力的精原細(xì)胞，用于體外培養(yǎng)。資料表明，從成年嚙齒類的睪丸分離粗線期及其后各發(fā)育階段生精細(xì)胞的工作已取得可喜成績，所獲細(xì)胞的純度已超過90%，產(chǎn)量達(dá)到107個(gè)。但可用于體外培養(yǎng)的精原細(xì)胞的分離卻一直進(jìn)展不大。有些報(bào)道雖然純度很高，產(chǎn)量卻很低；另一些報(bào)道產(chǎn)量高但純度低。1977年，Bellve等報(bào)道從生后8d小鼠睪丸中獲得了純度91%的A型精原細(xì)胞，產(chǎn)量達(dá)2×107個(gè)。但使用的小鼠卻多達(dá)60余只，耗費(fèi)了大量的實(shí)驗(yàn)動(dòng)物。我們的前期工作已表明，小鼠生后7～8d，其生精上皮內(nèi)基本只有支持細(xì)胞和A型精原細(xì)胞，其他各級(jí)生精細(xì)胞尚未發(fā)育形成。在體外，支持細(xì)胞總是殖細(xì)胞而貼壁。因此，本實(shí)驗(yàn)選用生后7～8d小鼠的睪丸，事先排除其他生精細(xì)胞的污染，用組合酶消化獲得細(xì)胞懸液，經(jīng)Percoll不連續(xù)密度梯度離心，再用選擇性貼壁法純化，獲得了較高純度的精原細(xì)胞，基本滿足了體外培養(yǎng)的需要。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

想了解更多的產(chǎn)品及服務(wù)請掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場部】     歐

【】 
【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

principle:
Sudan dyes are more soluble in lipids than their solubility in solvents. If the dye comes into contact with lipid-containing samples, a large amount of dye enters the structure of the lipid and renders the structures red. Multi Sudan dye, oil red O, Nile blue and other lipid-soluble dye staining, the lipid color. Can also be used antigen staining, fatty acids or choline can make the antigen antibody reduced to osmium black and black.
Preparation of reagents:
1. Sudan Ⅲ staining solution: 1g Sudan Ⅲ dissolved in 70% ethanol solution was filtered and reserved;
Formaldehyde calcium fixation solution: 10ml of formaldehyde and 1g CaCl2 mixed with water to 100ml;
3. Glycerin gelatin: Add 12g gelatin to 120ml distilled water to make it compley dissolved in water bath, then add 60ml glycerol and 1g phenol, and mix well to get it. Store at 4 ° C for later use
Dyeing steps:
1. Rinse the coverslip 3 times with Hanks solution;
2. Formaldehyde calcium fixative fixed 20min;
Rinse cover glass 3 times with distilled water;
4. Rinse cover glass 3 times with 70% ethanol;
Cover the sample with Sudan Ⅲ stained solution for 10-30min;
6. Rinse the cover 3 times with 70% ethanol;
7. After viewing with glycerol gelatin;
result:
Primary cells contain lipids in the area of ??orange-red;
Spermatogonial physiological and biochemical characteristics and its division of proliferation, differentiation, exercise, aging, death and other life-cycle regulatory mechanisms in-depth study of antigen and antibody pathogenesis further elucidation and spermatogonial alloantigen and antigenic antibody technology practical application , There is an urgent need to find a viable method to separate and purify them in order to obtain a higher yield and purity of viable spermatogonia for in vitro culture. Data show that the adult rodents from the testes pachytene and subsequent stages of development of spermatogenic cells work has made gratifying achievements, the purity of the cells obtained by more than 90%, the output reached 107 [1]. However, the isolation of spermatogonia, which can be used for in vitro culture, has been little progress. Some reported high purity but low yields; others reported high yields but low purity. In 1977, Bellve et al. [2] reported that 91% of A-type spermatogonia were obtained from the testes of mice 8 days after birth, with a yield of 2 × 107. However, as many as 60 mice were used, consuming a large amount of experimental animals. Our preliminary work has shown that mice have only basic supportive cells and type A spermatogonia within 7-8 days after birth, and other types of spermatogenic cells have not yet developed [3]. In vitro, the supporting cells are always adherent to the germ cells. Therefore, in this experiment, the testis of 7 ~ 8 days after birth was used to exclude the contamination of other spermatogenic cells in advance. The cell suspension was obtained by digestion with combinatorial enzyme. The cells were centrifuged by discontinuous Percoll gradient centrifugation and purified by selective adherence. Obtained a higher purity of spermatogonia, basically meet the needs of in vitro culture.