急性淋巴細(xì)胞白血病抗原CD38

廣州健侖生物科技有限公司

CD38是一種II型穿膜糖蛋白，分子量為46kDa。盡管zui初CD38是做為T細(xì)胞分化抗原而被發(fā)現(xiàn)的， 但是近年的研究表明它具有廣泛的細(xì)胞和組織分布。 CD38表達(dá)于胸腺細(xì)胞、前B細(xì)胞、生發(fā)中心B細(xì)胞、漿細(xì)胞、單核細(xì)胞、NK細(xì)胞、絲裂原活化的T細(xì)胞等細(xì)胞，可用于急性白血病的分型和活化的 T 細(xì)胞在自身免疫和免疫缺損中的作用等方面的研究，也可作為生發(fā)中心 B 細(xì)胞的一種選擇性參考依據(jù)。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞膜

克隆號：SP149

同型：IgG1

適用組織：石蠟/冰凍

陽性對照：扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

急性淋巴細(xì)胞白血病抗原CD38

在2升磨口回流瓶中，加入100克鎢酸鈉（Na2WO4·2H2O）,25克鉬酸鈉（Na2MoO4·2H2O）及700毫升蒸餾水，再加50毫升85%磷酸，100毫升濃鹽酸，充分混合，接上回流管，以小火回流10小時(shí)，回流結(jié)束時(shí)，加入150克硫酸鋰（Li2SO4），50毫升蒸餾水及數(shù)滴液體溴，開口繼續(xù)沸騰15分鐘，以便驅(qū)除過量的溴。冷卻后溶液呈黃色（如仍呈綠色，須再重復(fù)滴加液體溴的步驟）。稀釋至1升，過濾，濾液置于棕色試劑瓶中保存。使用時(shí)用標(biāo)準(zhǔn)NaOH滴定，酚酞作指示劑，然后適當(dāng)稀釋，約加水1倍，使zui終的酸濃度為1N左右。
（3）標(biāo)準(zhǔn)蛋白質(zhì)溶液:
精確稱取結(jié)晶牛血清清蛋白或g—球蛋白，溶于蒸餾水，濃度為250 mg/ml左右。牛血清清蛋白溶于水若混濁，可改用0.9 % NaCl溶液。
2.器材
（1）可見光分光光度計(jì)（2）旋渦混合器（3）秒表（4）試管16支
（三）操作方法
1.標(biāo)準(zhǔn)曲線的測定：取16支大試管，1支作空白，3支留作未知樣品，其余試管分成兩組，分別加入0，0.1，0.2，0.4，0.6，0.8，1.0毫升標(biāo)準(zhǔn)蛋白質(zhì)溶液（濃度為250mg/ml）。用水補(bǔ)足到1.0毫升，然后每支試管加入5毫升試劑甲，在旋渦混合器上迅速混合，于室溫（20～25℃）放置10分鐘。再逐管加入0.5毫升試劑乙（Folin—酚試劑），同樣立即混勻。這一步混合速度要快，否則會使顯色程度減弱。然后在室溫下放置30分鐘，以未加蛋白質(zhì)溶液的*支試管作為空白對照，于700nm處測定各管中溶液的吸光度值。以蛋白質(zhì)的量為橫座標(biāo)，吸光度值為縱座標(biāo)，繪制出標(biāo)準(zhǔn)曲線。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

100g of sodium tungstate (Na2WO4 · 2H2O), 25g of sodium molybdate (Na2MoO4 · 2H2O) and 700ml of distilled water were added into a 2 liter mill mouth reflux bottle, and then 50ml of 85% phosphoric acid and 100ml of concentrated hydrochloric acid were added sufficiently Mixed, connected to a reflux tube and refluxed for 10 hours with a small flame. At the end of the refluxing, 150 g of lithium sulfate (Li2SO4), 50 ml of distilled water and a few drops of liquid bromine were added and the opening continued to boil for 15 minutes in order to repel excess bromine. After cooling the solution is yellow (if still green, to be repeated dropwise addition of liquid bromine step). Dilute to 1 liter, filter, and store filtrate in brown reagent bottle. Use standard NaOH titration, phenolphthalein as an indicator, and then appropriate dilution, add about 1 times the water, so that the final acid concentration of about 1N.
(3) standard protein solution:
Accuray weigh crystalline bovine serum albumin or g-globulin, dissolved in distilled water at a concentration of about 250 mg / ml. Bovine serum albumin soluble in water if cloudy, can be used to 0.9% NaCl solution.
2. Equipment
(1) Visible spectrophotometer (2) Vortex mixer (3) Stopwatch (4) Test tube 16
(C) method of operation
1. Determination of the standard curve: Take 16 large test tubes, one for the blank, three for unknown samples, the remaining test tube into two groups were added 0,0.1,0.2,0.4,0.6,0.8,1.0 ml standard protein solution (At a concentration of 250 mg / ml). Make up to 1.0 ml with water, then add 5 ml Reagent A per tube, mix rapidly on a vortex mixer and allow to stand at room temperature (20-25 ° C) for 10 minutes. And then by tube by adding 0.5 ml of reagent B (Folin-phenol reagent), the same immediay mix. This step mix faster, otherwise it will make the color less. Then, it was left to stand for 30 minutes at room temperature. The first tube without any protein solution was used as blank control, and the absorbance value of the solution in each tube was measured at 700 nm. Take the amount of protein as the abscissa and absorbance as the ordinate, and draw a standard curve.