CD34內(nèi)皮細胞標記（鼠單克隆抗體）

廣州健侖生物科技有限公司

CD34 是一種分子量為110kDa 的單鏈跨膜蛋白，表達于早期淋巴造血干細胞、祖細胞、血管內(nèi)皮細胞、胚胎纖維母細胞和某些神經(jīng)組織的細胞。超過85％的血管肉瘤和Kaposi’s 肉瘤CD34 表達陽性；CD34 與CD117 聯(lián)合用于研究GIST；孤立性纖維性腫瘤CD34 亦陽性表達。該抗體主要用于良/惡性血管內(nèi)皮腫瘤、GIST、孤立性纖維性腫瘤的研究。

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

歡迎咨詢

歡迎咨詢

【產(chǎn)品介紹】

細胞定位：細胞膜

克隆號：QBEnd/10

同型：IgG1

適用組織：石蠟/冰凍

陽性對照：扁桃體/血管內(nèi)皮

抗原修復：熱修復（EDTA）

抗體孵育時間：30-60min

CD34內(nèi)皮細胞標記（鼠單克隆抗體）

（2）加完試劑2~5分鐘后，即可開始用比色皿，在分光光度計上測定各樣品在595nm處的光吸收值A(chǔ)595，空白對照為第1號試管，即0.1mlH2O加5.0mlG―250試劑。
注意：不可使用石英比色皿（因不易洗去染色），可用塑料或玻璃比色皿，使用后立即用少量95%的乙醇蕩洗，以洗去染色。塑料比色皿決不可用乙醇或丙酮長時間浸泡。
（3）用標準蛋白質(zhì)量（mg）為橫座標，用吸光度值A(chǔ)595為縱座標，作圖，即得到一條標準曲線。由此標準曲線，根據(jù)測出的未知樣品的A595值，即可查出未知樣品的蛋白質(zhì)含量。
0.5mg牛血清蛋白/ml溶液的A595約為0.50。
2. 微量法
當樣品中蛋白質(zhì)濃度較稀時（10－100mg/ml）,可將取樣量（包括補加的水）加大到0.5ml或1.0ml, 空白對照則分別為0.5ml或1.0ml H2O, 考馬斯亮藍G－250試劑仍加5.0ml, 同時作相應(yīng)的標準曲線，測定595nm的光吸收值。0.05mg牛血清蛋白/ml溶液的A595約為0.29。
（一）實驗原理
這種蛋白質(zhì)測定法是zui靈敏的方法之一。過去此法是應(yīng)用zui廣泛的一種方法，由于其試劑乙的配制較為困難（現(xiàn)在已可以訂購），近年來逐漸被考馬斯亮蘭法所取代。此法的顯色原理與雙縮脲方法是相同的，只是加入了第二種試劑，即Folin—酚試劑，以增加顯色量，從而提高了檢測蛋白質(zhì)的靈敏度。這兩種顯色反應(yīng)產(chǎn)生深蘭色的原因是：?在堿性條件下，蛋白質(zhì)中的肽鍵與銅結(jié)合生成復合物。Folin—酚試劑中的磷鉬酸鹽—磷鎢酸鹽被蛋白質(zhì)中的酪氨酸和苯丙氨酸殘基還原，產(chǎn)生深蘭色（鉬蘭和鎢蘭的混合物）。在一定的條件下，蘭色深度與蛋白的量成正比。

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

想了解更多的產(chǎn)品及服務(wù)請掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

【】 
【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

(2) After adding reagent for 2 to 5 minutes, begin to use the cuvette, and measure the light absorption value A595 of each sample at 595 nm on the spectrophotometer, the blank control is No. 1 test tube, ie 0.1 ml H 2 O plus 5.0 mlG-250 reagent.
Note: Do not use quartz cuvettes (not easy to wash to remove staining), available plastic or glass cuvette, immediay after use with a small amount of 95% ethanol wash to wash away the stain. Plastic cuvettes must never be soaked in ethanol or acetone for a long time.
(3) Using the standard protein mass (mg) as the abscissa and the absorbance value A595 as the ordinate for mapping, a standard curve is obtained. From this standard curve, the protein content of an unknown sample can be determined based on the A595 value of the unknown sample measured.
The A595 of 0.5 mg bovine serum albumin / ml solution is about 0.50.
2. Trace method
When the protein concentration in the sample is relatively low (10-100mg / ml), the sample volume (including additional water) can be increased to 0.5ml or 1.0ml, while the blank control is respectively 0.5ml or 1.0ml H2O, Coomassie bright Blue G-250 reagent is still added 5.0ml, at the same time as the corresponding standard curve, measuring the light absorption value of 595nm. The A595 of 0.05 mg bovine serum albumin / ml solution was about 0.29.
(A) experimental principle
This protein assay is one of the most sensitive methods. In the past, this method was the most widely used one. Due to the difficulty in formulating its reagent B (which can now be ordered), it has gradually been replaced by Coomassie brilliant blue method in recent years. This method of color principle and biuret method is the same, but added a second reagent, Folin-phenol reagent, in order to increase the amount of color, thereby enhancing the detection of protein sensitivity. The reason for the dark blue color produced by these two color reactions is: • Under alkaline conditions, peptide bonds in the protein bind to copper to form complexes. Phosphomolybdate-phosphotungstate in the Folin-phenol reagent is reduced by tyrosine and phenylalanine residues in the protein to produce dark blue (a mixture of molybdenum and tungsten). Under certain conditions, the depth of blue is proportional to the amount of protein.