CD23(B細胞)鼠單克隆抗體

廣州健侖生物科技有限公司

CD23 是一種分子量為 45KDa的細胞膜表面糖蛋白，是存在于 B細胞表面的低親和性 IgE 受體。表達于外周血細胞的某些亞群、 B淋巴細胞、淋巴濾泡樹突細胞(FDC)、初始淋巴細胞和 EB 病毒轉染的 B 淋巴瘤細胞系。慢性淋巴細胞白血病和小淋巴細胞性淋巴瘤瘤細胞也表達CD23，但套細胞淋巴瘤不表達 CD23。

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細胞定位：細胞膜

克隆號：MRQ-57

同型：IgG1/K

適用組織：石蠟/冰凍

陽性對照：扁桃體

抗原修復：熱修復（EDTA）

抗體孵育時間：30-60min

CD23(B細胞)鼠單克隆抗體

測定微生物細胞數(shù)量的方法很多，通常采用的有顯微鏡直接計數(shù)法和稀釋平板計數(shù)法。
直接計數(shù)法適用于各種單細胞菌體的純培養(yǎng)懸浮液，如有雜菌或雜質(zhì)，則難于直接測定。菌體較大的酵母菌或霉菌孢子可采用血球計數(shù)板，一般細菌則采用彼德羅夫·霍澤（Petrof Hausser）細菌計數(shù)板。兩種計數(shù)板的原理和部件相同，只是細菌計數(shù)板較薄，可以使用油鏡觀察。而血球計數(shù)板較厚，不能使用油鏡，計數(shù)板下部的細菌難于區(qū)分。
血球計數(shù)板是一塊特制的厚型載玻片，載坡片上有4條槽所構成的3個平臺。中間的平臺較寬，其中間又被一短橫槽分隔成兩半，每個半邊上面各有一個計數(shù)區(qū)（圖示），計數(shù)區(qū)的刻度有兩種：一種是計數(shù)區(qū)分為16個大方格，大方格用三線隔開，而每個大方格又分成25個小方格；另一種是一個計數(shù)區(qū)分成25個大方格，大方格之間用雙線分開，而每個大方格又分成16個小方格。但是不管計數(shù)區(qū)是哪一種構造，它們都有一個共同特點，即計數(shù)區(qū)都由400個小方格組成（圖示）。
計數(shù)區(qū)邊長為1mm，則計數(shù)區(qū)的面積為1mm2，每個小方格的面積為1/400 mm2。蓋上蓋玻片后，         
計數(shù)區(qū)的高度為0.1mm，所以計數(shù)區(qū)的體積為0.1mm3，每個小方格的體積為1/4000 mm3。
使用血球計數(shù)板計數(shù)時，先要測定每個小方格中微生物的數(shù)量，再換算成每mL菌液（或每g樣品）中微生物細胞的數(shù)量。
已知：1mL體積＝10 mm×10 mm×10 mm＝1000mm3       
所以：1mL體職應含有小方格數(shù)為1000mm3/（1/4000mm3）＝4×106個小方格，即系數(shù)K＝4×106。
因此：每mL菌懸液中含有細胞數(shù)＝每個小格中細胞平均數(shù)（N）×系數(shù)（K）×菌液稀釋倍數(shù)（d）。
六、實驗材料
活材料：釀酒酵母（Saccharomyces cerevisiae）斜面菌種或培養(yǎng)液、枯草桿菌（Bacillus subtilis）染色標本片。

CD23(B細胞)鼠單克隆抗體

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

想了解更多的產(chǎn)品及服務請掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

There are many ways to determine the number of microbial cells, usually the direct counting microscope and the dilution plate counting method.
Direct counting method for a variety of single cell suspension of pure culture, such as bacteria or impurities, it is difficult to direct determination. Larger cell strains of yeast or mold spores can be used blood count plate, the general bacteria are used Petrof Hausser bacteria count plate. The principle and components of the two counting plate the same, but the bacterial count plate is thin, you can use oil mirror observation. The blood count plate thicker, can not use the oil mirror, the lower part of the counting plate difficult to distinguish bacteria.
The blood cell counting plate is a special thick glass slide, which contains 3 platforms formed by 4 slots. The middle of the platform wider, the middle was a short groove separated by two halves, each half of each have a counting zone (Figure), there are two counting zone scale: a count is divided into 16 large Checkered, large squares separated by three lines, and each large square is divided into 25 small squares; the other is a count is divided into 25 large square, large squares separated by two lines, and Each big square is divided into 16 small squares. But regardless of the construction of the counting zone, they all have one thing in common: the counting zone consists of 400 small squares (pictured).
Counting area edge length is 1mm, then counting area area of ??1mm2, each small square area of ??1 / 400mm2. After covering the cover glass,
The height of the counting zone is 0.1 mm, so the volume of the counting zone is 0.1 mm3 and the volume of each small square is 1/4000 mm3.
When counting with a hemocytometer, the number of microbes in each small square is determined and then converted into the number of microbial cells per mL of broth (or per gram of sample).
It is known that 1 mL volume = 10 mm × 10 mm × 10 mm = 1000 mm 3
So: 1mL bodywork should contain a small number of squares 1000mm3 / (1 / 4000mm3) = 4 × 106 small squares, the coefficient K = 4 × 106.
Therefore: Number of cells contained in each mL of bacterial suspension = average number of cells in each cell (N) x coefficient (K) x bacterial dilution (d).
Six, experimental materials
Live material: Saccharomyces cerevisiae benthic strain or culture, Bacillus subtilis stained specimen.