CD21(B細胞)兔單克隆抗體

廣州健侖生物科技有限公司

CD21 又稱 2 型補體受體（complement receptor type 2，CR2）和 EB 病毒受體，是補體激活調節(jié)劑家族的一員。CD21主要分布在成熟的 B細胞、淋巴濾泡內的樹突細胞、部分 T 細胞。CD21 的功能有促進 細胞增殖，介導 EBV 轉化 B 細胞，參與免疫記憶。主要用于濾泡狀細胞瘤和免疫補體激活調節(jié)的研究。

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

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【產品介紹】

細胞定位：細胞膜/細胞漿

克隆號：EP3093

同型：IgG

適用組織：石蠟/冰凍

陽性對照：扁桃體

抗原修復：熱修復（EDTA）

抗體孵育時間：30-60min

CD21(B細胞)兔單克隆抗體

6、化學藥劑對微生物的作用
⑴制平板：取無菌平皿3套，將已熔化并冷卻至50℃左右的抗原抗體蛋白胨瓊脂培養(yǎng)基按無菌操作法倒入平皿中，使冷凝成平板。
⑵制備菌懸液：取無菌水3支，用接種環(huán)分別取大腸桿菌、金黃色葡萄球菌和枯草桿菌各1～2環(huán)接入無菌水中，充分混勻，制成菌懸液。
⑶接種：用無菌吸管分別吸取已制好的菌懸液0.1mL接種于平板上，用無菌玻璃刮鏟涂勻。注意做好標記。
⑷浸藥：將滅菌濾紙片浸入供試藥劑中。
⑸加藥劑：用無菌鑷子夾取浸藥濾紙片，注意把藥液瀝干，分別平鋪于同一含菌平
板上，注意藥劑之間勿互相沾染并在平皿背面做好標記。
⑹培養(yǎng)：將平皿置于28℃下培養(yǎng)48～72h后觀察結果。
實驗原理
微生物細胞的大小是微生物重要的形態(tài)特征之一，由于菌體微小，只能在顯微鏡下測量。用于測量微生物細胞大小的工具有目鏡測微尺和鏡臺測微尺。
目鏡測微尺（圖示）是一塊圓形玻片，在玻片中央把5mm長度刻成50等分，或把10mm長度刻成100等分。測量時，將其放在接目鏡中的隔板上，此處正好與物鏡放大的中間物像重迭，用于測量經顯微鏡放大后的細胞物象。由于不同目鏡、物鏡組合的放大倍數(shù)不相同，目鏡測微尺每格實際表示的長度也不一樣，因此目鏡測微尺測量微生物大小時須先用置于鏡臺上的鏡臺測微尺校正，以求出在一定放大倍數(shù)下，目鏡測微尺每小格所代表的相對長度。鏡臺測微尺（圖示）是中央部分刻有精確等分線的載玻片，一般將1mm等分為100格，每格長10μm即0.01mm，是專門用來校正目鏡測微尺的。校正時，將鏡臺測微尺放在載物臺上，由于鏡臺測微尺與細胞標本是處于同一位置，都要經過物鏡和目鏡的兩次放大成像進入視野，即鏡臺測微尺隨著顯微鏡總放大倍數(shù)的放大而放大，因此從鏡臺測微尺上得到的讀數(shù)就是細胞的真實大小，所以用鏡臺測微尺的已知長度在一定放大倍數(shù)下校正目鏡測微尺，即可求出目鏡測微尺每格所代表的實際長度，然后移去鏡臺測微尺，換上待測標本片，用校正好的目鏡測微尺在同樣放大倍數(shù)下測量微生物細胞大小。

CD21(B細胞)兔單克隆抗體

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

想了解更多的產品及服務請掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

6, the role of chemical agents on microorganisms
⑴ system plate: Take three sets of sterile dishes, will have been melted and cooled to about 50 ℃ antigen antibody peptone agar medium according to aseptic method into the plate, so that the condensation into the plate.
⑵ Preparation of bacterial suspension: take sterile water 3, with the inoculation loop were taken Escherichia coli, Staphylococcus aureus and Bacillus subtilis 1 ~ 2 loop access to sterile water, mix well to make bacterial suspension.
(3) Inoculation: Use sterile pipette respectively to absorb 0.1mL of prepared bacterial suspension and inoculate them on the plate, and spread them evenly with a sterile glass spatula. Take care to mark it.
⑷ dip medicine: sterile filter paper immersed in the test agent.
⑸ add medicine: with sterile tweezers grip leaching filter paper, pay attention to drain the liquid, respectively, tiled in the same bacteria level
On the board, pay attention to the drug do not contaminate each other and mark the back of the plate.
⑹ culture: the plate was placed at 28 ℃ for 48 ~ 72h after the observation.
Experimental principle
Microbial cell size is one of the important morphological characteristics of microorganisms, due to the tiny cells can only be measured under a microscope. Tools for measuring microbial cell size include eyepiece micrometer and stage micrometer.
The eyepiece micrometer (pictured) is a round slide with a length of 5 mm engraved into the center of the slide, or a 10 mm length cut into 100 equal parts. When measured, it is placed on the partition in the eyepiece, where it overlaps with the magnified intermediate image of the objective to measure the magnified cellular image. Because different eyepiece, objective combination of magnification is not the same, eyepiece micrometer ruler each grid actually expressed length is not the same, so the eyepiece micrometer ruler to measure the size of the microorganism must be placed on the stage with a microscope micrometer ruler to Obtained at a certain magnification, eyepiece micrometer scale each cell represents the relative length. The microscope micrometer scale (pictorial) is a special glass slide engraved with precise bisector in the central part. It usually divides 1mm equally into 100 cells, each 10μm long or 0.01mm, which is designed to correct the eyepiece micrometer . Calibration, the microscope micrometer ruler placed on the stage, as the stage micrometer ruler and cell specimens are in the same position, both through the objective lens and eyepiece magnification into the field of vision two times, that mirror stage micrometer with the microscope The total magnification of amplification and amplification, so the micrometer from the stage to get the reading is the true size of the cell, so the known length of the mirror with a micrometer scale known magnification correction eyepiece micrometer, you can find the eyepiece Measure the actual length of each cell represented by the micrometer and then remove the micrometer from the stage and place the specimen under test with a calibrated eyepiece micrometer to measure microbial cell size at the same magnification.