CD20(B細胞)鼠單克隆抗體

廣州健侖生物科技有限公司

CD20主要分布于前B細胞后期、B細胞和漿細胞前的B細胞。CD20（L26）能識別外周血和淋巴組織的B細胞，在正常淋巴組織中，L26可作為生發(fā)中心的B細胞的參考依據(jù)，特別是免疫母細胞。此抗體用于B淋巴細胞及B細胞淋巴瘤的研究。

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

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【產品介紹】

細胞定位：細胞膜

克隆號：L26

同型：IgG2a/K

適用組織：石蠟/冰凍

陽性對照：扁桃體

抗原修復：熱修復（EDTA）

抗體孵育時間：30-60min

CD20(B細胞)鼠單克隆抗體

4、氫離子濃度對微生物生長的影響
⑴培養(yǎng)基制備：分組按下列配方配制不同pH值的培養(yǎng)基，并用pH計校證pH值，然后分裝入試管中，每管裝量5～6 mL,0.1Mpa滅菌30min備用，見表。
⑵接種培養(yǎng)：取供試pH培養(yǎng)基三組用接種環(huán)按無菌操作法于試管中分別接入大腸桿菌、吸水鏈霉菌、黑曲霉。在37℃下培養(yǎng)48h。
⑶檢查結果：出取培養(yǎng)物，觀察并記錄實驗結果。
5、紫外線殺菌試驗
⑴制平板：取無菌平皿6套，將已熔化并冷卻至50℃左右的抗原抗體蛋白胨瓊脂培養(yǎng)基
⑵菌懸液制備：取試管無菌水2支，以無菌操作法分別取金黃色葡萄球菌和黏質沙雷氏菌各2環(huán)，接入無菌水中充分搖勻，制成菌懸液。
⑶接種：將已倒入培養(yǎng)基的平皿分成2組，每組3個，一組接金黃色葡萄球菌，一組接沙雷氏菌，用無菌吸管吸取已制好的菌懸液各0.1 mL，分別在接種于二組平板上，用無菌玻璃刮鏟布均涂勻，隨即用無菌鑷子夾取無菌圖案紙一張，小心放在接種好的平皿中央。
⑷分組：將接種的六個平皿分為三組，每組一個金黃色葡萄球菌，一個黏質沙雷氏菌。
⑸紫外線處理：將紫外燈先開燈預熱2～3min。再將上述平皿置于紫外燈下，打開皿蓋，在30cm距離處照射。一組照1min，1組照5min，1組照10min，小心地取下圖案紙，蓋上皿蓋。用黑布或厚紙遮蓋，送入培養(yǎng)室內?！锎藭r能否不進行遮蓋處理，直接拿出來培養(yǎng)？
⑹培養(yǎng)：將平皿于28～30℃溫度下培養(yǎng)48h。
⑺觀察：兩菌在不同的處理條件下的生長狀況，即有無生長、生長數(shù)量多少、生長的位置等。

CD20(B細胞)鼠單克隆抗體

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

4, hydrogen ion concentration on microbial growth
Preparation of medium: Divide the medium with different pH values ??according to the following formula and calibrate the pH value with pH meter. Then dispense it into the test tube. The volume of each medium is 5 ~ 6 mL and sterilize with 0.1Mpa for 30min. .
⑵ inoculation culture: take test pH medium three groups with vaccination ring according to aseptic method were in vitro access to Escherichia coli, Streptomyces hygroscopicus, Aspergillus niger. Incubate at 37 ° C for 48h.
⑶ test results: take the culture, observe and record the experimental results.
5, UV disinfection test
⑴ system plate: take sterile plate six sets, will have been melted and cooled to about 50 ℃ antigen antibody peptone agar medium
⑵ bacterial suspension preparation: Take sterile tube of test tube 2, respectively, to take aseptic method of Staphylococcus aureus and Serratia marcescens 2, access to sterile water shake well, made of bacterial suspension .
⑶ inoculation: the plate has been poured into the medium into two groups, each group of three, a group of Staphylococcus aureus, a group of Serratia bacteria, with a sterile pipette system prepared bacterial suspension 0.1 mL, were inoculated on two sets of plates, with a sterile glass scraping cloth are evenly coated, then sterile tweezers clip a sterile pattern paper, carefully placed inoculated good dish center.
⑷ grouping: The inoculated six dishes were divided into three groups, each with a Staphylococcus aureus, a serratia marcescens.
⑸ UV treatment: the UV light to turn on the lights preheat 2 ~ 3min. Then the above plate under UV light, open the dish cover, at a distance of 30cm irradiation. One group according to 1min, 1 group according to 5min, 1 group according to 10min, carefully remove the pattern paper, cover the lid. Covered with black cloth or thick paper, into the training room. ★ At this point can not be covered, directly brought up to train?
⑹ Culture: The plate at 28 ~ 30 ℃ temperature for 48h.
⑺ observation: two bacteria in different processing conditions of growth, that is, with or without growth, the number of growth, the location of growth and so on.