鼠抗人BOB.1單克隆抗體

廣州健侖生物科技有限公司

BOB.1又稱OCA-B或OBF-1，是B淋巴細(xì)胞的特異性核轉(zhuǎn)錄因子協(xié)調(diào)刺激物，是B細(xì)胞免疫球蛋白基因表達(dá)所需要的轉(zhuǎn)錄因子，通常表達(dá)在B淋巴細(xì)胞核中，包括漿細(xì)胞。BOB.1顯示出B細(xì)胞特異表達(dá)模式，與B細(xì)胞的活化及發(fā)生中心的形成有密切關(guān)系。同時(shí)，BOB.1在T淋巴細(xì)胞也具有基因轉(zhuǎn)錄調(diào)節(jié)的作用?？贵wBOB.1常用于B細(xì)胞淋巴瘤和結(jié)節(jié)樣淋巴細(xì)胞性何杰金淋巴瘤的診斷。一些T細(xì)胞淋巴瘤亦可出現(xiàn)BOB.1陽性。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞核

克隆號：MRQ-35

同型：IgG2b

適用組織：石蠟/冰凍

陽性對照：B細(xì)胞淋巴瘤/霍奇金氏淋巴瘤/扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

更高級的應(yīng)用主要有以下幾個方面：
因釀酒酵母與同為真核生物的動物和植物細(xì)胞具有很多相同的結(jié)構(gòu)，又容易培養(yǎng)，酵母被用作研究真核生物的模式生物，也是目前被人們了解zui多的生物之一。在人體中重要的蛋白質(zhì)很多都是在酵母中先被發(fā)現(xiàn)其同源物的，其中包括有關(guān)細(xì)胞周期的蛋白、信號蛋白和蛋白質(zhì)加工酶。
釀酒酵母也是制作培養(yǎng)基中常用成分酵母提取物的主要原料。
酵母作為高等真核生物特別是人類基因組研究的模式生物，其zui直接的作用體現(xiàn)在生物信息學(xué)領(lǐng)域。當(dāng)人們發(fā)現(xiàn)了一個功能未知的人類新基因時(shí)，可以迅速地到任何一個酵母基因組數(shù)據(jù)庫中檢索與之同源的功能已知的酵母基因，并獲得其功能方面的相關(guān)信息，從而加快對該人類基因的功能研究。研究發(fā)現(xiàn)，有許多涉及遺傳性疾病的基因均與酵母基因具有很高的同源性，研究這些基因編碼的蛋白質(zhì)的生理功能及它們與其它蛋白質(zhì)之間的相互作用將有助于加深對這些遺傳性疾病的了解。此外，人類許多重要的疾病，如早期糖尿病、小腸癌和心臟疾病，均是多基因遺傳性疾病，揭示涉及這些疾病的所有相關(guān)基因是一個困難而漫長的過程，酵母基因與人類多基因遺傳性疾病相關(guān)基因之間的相似性將為人類提高診斷和治療水平提供重要的幫助。
酵母作為模式生物的例子體現(xiàn)在那些通過連鎖分析、定位克隆然后測序驗(yàn)證而獲得的人類遺傳性疾病相關(guān)基因的研究中，后者的核苷酸序列與酵母基因的同源性為其功能研究提供了*的線索。例如，人類遺傳性非息肉性小腸癌相關(guān)基因與酵母的MLH1、MSH2基因，運(yùn)動失調(diào)性毛細(xì)血管擴(kuò)張癥相關(guān)基因與酵母的1基因，布盧姆氏綜合征相關(guān)基因與酵母的SGS1基因，都有很高的同源性。遺傳性非息肉性小腸癌基因在腫瘤細(xì)胞中表現(xiàn)出核苷酸短重復(fù)順序不穩(wěn)定的細(xì)胞表型，而在該人類基因被克隆以前，研究工作者在酵母中分離到具有相同表型的基因突變（msh2和mlh1突變）。受這個結(jié)果啟發(fā)，人們推測小腸癌基因是MSH2和MLH1的同源基因，而它們在核苷酸序列上的同源性則進(jìn)一步證實(shí)了這一推測。布盧姆氏綜合征是一種臨床表現(xiàn)為性早熟的遺傳性疾病，病人的細(xì)胞在體外培養(yǎng)時(shí)表現(xiàn)出生命周期縮短的表型，而其相關(guān)基因則與酵母中編碼蝸牛酶的SGS1基因具有很高的同源性。與來自布盧姆氏綜合征個體的培養(yǎng)細(xì)胞相似，SGS1基因突變的酵母細(xì)胞表現(xiàn)出顯著縮短的生命周期。Francoise等研究了170多個通過功能克隆得到的人類基因，發(fā)現(xiàn)它們中有42%與酵母基因具有明顯的同源性，這些人類基因的編碼產(chǎn)物大部分與信號轉(zhuǎn)導(dǎo)途徑、膜運(yùn)輸或者DNA合成與修復(fù)有關(guān)，而那些與酵母基因沒有明顯同源性的人類基因主要編碼一些膜受體、血液或免疫系統(tǒng)組分，或人類特殊代謝途徑中某些重要的酶和蛋白質(zhì)。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

More advanced applications mainly in the following areas:
Because Saccharomyces cerevisiae has many of the same structures as eukaryotic animals and plant cells and is easy to grow, yeast is used as a model organism for the study of eukaryotes and is one of the most widely understood organisms. Many of the proteins that are important in the body are first found in yeast, including the cell cycle-related proteins, signaling proteins and protein processing enzymes.
Saccharomyces cerevisiae is also the main raw material for producing yeast extract which is commonly used in the culture medium.
Yeast, as a model organism for higher eukaryotes, especially human genomics, has the most direct role in bioinformatics. When one finds a new human gene of unknown function, one can quickly search any one of the yeast genome databases for homologous, well-known yeast genes and gain information on their function to speed up the human Functional studies of genes. The study found that many genes involved in genetic diseases share high homology with yeast genes and that studying the physiological functions of the proteins encoded by these genes and their interactions with other proteins will help deepen the understanding of these genetic Understanding of sexually transmitted diseases. In addition, many important human diseases, such as early stage diabetes, small intestine cancer and heart disease, are polygenic inherited diseases. It is a difficult and lengthy process to reveal all the related genes involved in these diseases. Yeast gene and human polygenic inheritance The similarity between disease-related genes will provide important help for human to improve the level of diagnosis and treatment.
The best examples of yeast as a model organism are embodied in studies of human genetic disease-related genes obtained by linkage analysis, positional cloning and sequencing validation, whose homology to the yeast genes is their function Research provides excellent clues. For example, human hereditary non-polyposis colorectal cancer-related genes and yeast MLH1, MSH2 gene, motor-related angiectasia related genes and yeast 1 gene, Bloom syndrome-related genes and yeast SGS1 gene, both High homology. Hereditary nonpolyposis colorectal cancer genes exhibit a short-stranded nucleotide sequence that is unstable in tumor cells, and before the human gene was cloned, researchers isolated genes with the same phenotype in yeast Mutations (msh2 and mlh1 mutations). Inspired by this result, it is speculated that the small intestine oncogene is a homologue of MSH2 and MLH1, and their homology to nucleotide sequences further confirms this conjecture. Bloom's syndrome is a hereditary disease characterized by precocious puberty. Cells from patients show a shortened life-cycle phenotype when cultured in vitro, while their genes are associated with the SGS1 gene encoding snail amylase in yeast High homology. Similar to the cultured cells from individuals with Bloom syndrome, SGS1 mutant yeast cells showed a significantly shortened life cycle. Francoise et al. Studied more than 170 human genes cloned by function and found that 42% of them have obvious homology with yeast genes. Most of the coding products of these human genes are involved in signal transduction pathway, membrane trafficking or DNA synthesis Is involved in repair, while those human genes that have no significant homology to the yeast gene encode primarily membrane receptors, components of the blood or immune system, or certain important enzymes and proteins in the special metabolic pathways of humans.