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Medium 199
sterility:sterile-filtered.
form:liquid.
technique(s):cell culture | mammalian: suitable.
impurities:endotoxin, tested.
components:L-glutamine: yes;phenol red: yes;HEPES: no;sodium pyruvate: no;NaHCO3: yes;Earle’s salts (5% CO2): yes.
General description:Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro c*tion of primary pancreatic explants, and lens tissues.