目錄:愛必信(上海)生物科技有限公司>>細胞生物學>>重組蛋白>> abs01102Recombinant Equine IFN-γ
供貨周期 | 一周 | 規(guī)格 | 20ug |
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概述 | |
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種屬 | Equine |
形態(tài) | Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4, with 5 % trehalose. |
來源 | Escherichia coli. |
氨基酸序列 | QAAFFKEIEN LKEYFNASNP DVGDGGPLFL DILKNWKEDS DKKIIQSQIV SFYFKLFENL KDNQVIQKSM DTIKEDLFVK FFNSSTSKLE DFQKLIQIPV NDLKVQRKAI SELIKVMNDL SPKANLRKRK RSQNPFRGRR ALQ |
內(nèi)毒素水平 | Less than 0.1 EU/µg of rEqIFN-γ as determined by LAL method. |
活性 | Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HeLa cells infected with encephalomyocarditis (EMC) virus is less than 10.0 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
Accession # | P42160 |
預(yù)期分子量 | Approximay 16.7 kDa, a single non-glycosylated polypeptide chain containing 143 amino acids. |
性能 | |
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溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
純度 | > 97 % by SDS-PAGE and HPLC analyses. |
Gene IDs | 100034181 |