蛋白免疫印跡實驗(Western Blot)實驗大家肯定都不陌生,要想做出好看的條帶,從制膠開始就不容半點馬虎。WB制膠可謂實驗室新手的必修課了,想想小編我在當(dāng)初實驗室的時候,就沒少幫師兄師姐配置WB凝膠,泛黃的配方表小編還記憶猶新。
WB凝膠配方表
說起WB凝膠配置,小編可有一肚子苦水要倒,讓人頭疼的一點就是多種溶液都需要計算量取,每次調(diào)移液器就要調(diào)半天。
還有各種試劑的保存條件也不一樣,配膠之前還需要從冰箱,室溫柜子里分別拿出不同的溶液。
這還不算,容易出問題的促凝劑APS,需要新鮮配置,4度保存不能超過一周,關(guān)鍵是粉末還特容易吸水,打開后需要密封保存。小編之前就踩過一次坑,APS粉末吸水結(jié)塊,稱量配置后的APS含量不夠,凝膠凝結(jié)不好,整個WB實驗條帶跑的七扭八歪。
TEMED就更不用說了,惡臭的氣味讓人很是不爽,每次打開都要做一下心理建設(shè)。
相信各位在實驗室奮戰(zhàn)的老師同學(xué)們肯定也是深有體會。小編在這里有個好消息告訴大家,愛必信上新品了,彩色凝膠試劑盒,完美解決WB制膠的以上痛點,快來看看吧~
彩色凝膠試劑盒特點:
1. 彩色上層膠:可制備紅色或綠色上層膠,加樣孔清晰易辨,方便上樣及區(qū)分不同凝膠。
2. 配膠過程短:可快速配置一塊或多塊彩色PAGE凝膠,無需復(fù)雜計算及配置,只需將等體積凝膠溶液及緩沖液混合,并加入少許促凝劑即可,操作簡便。
3. 穩(wěn)定且環(huán)保:促凝劑無TEMED成分,避免難聞臭味,并且更加穩(wěn)定,4度即可穩(wěn)定保存。凝膠均勻,彈性好,不易碎,電泳蛋白帶型美觀。
4. 條帶清晰:小分子蛋白條帶更清晰。
怎么樣,是不是心動了呢?選擇愛必信彩色凝膠試劑盒,讓您WB制膠不再難。小編再偷偷說一句,彩色凝膠和彩虹marker更配哦~
彩色凝膠試劑盒和彩虹蛋白marker相關(guān)產(chǎn)品推薦
貨號 | 品名 | 規(guī)格 | 目錄價 |
abs9365 | 彩色PAGE快速凝膠試劑盒(6%) | 1kit | 260 |
abs9366 | 彩色PAGE快速凝膠試劑盒(8%) | 1kit | 260 |
abs9367 | 彩色PAGE快速凝膠試劑盒(10%) | 1kit | 260 |
abs9368 | 彩色PAGE快速凝膠試劑盒(12%) | 1kit | 260 |
abs9369 | 彩色PAGE快速凝膠試劑盒(15%) | 1kit | 260 |
abs924 | 預(yù)染蛋白marker, 10-180kDa | 2×250ul | 372 |
abs923 | 預(yù)染蛋白marker | 500ul/5*500ul | 600/2400 |
蛋白質(zhì)印跡(Western Blot)實驗中產(chǎn)品推薦:
實驗步驟 | 推薦指數(shù) | 貨號 | 產(chǎn)品名稱 | 規(guī)格 |
樣本制備 | abs9228 | RIPA (中) | 100ml | |
abs9229 | RIPA (強) | 100ml | ||
abs9230 | RIPA (弱) | 100ml | ||
abs9231 | RIPA (強,無抑制劑) | 100ml | ||
abs9160 | NP-40 | 100ml | ||
abs9195 | 1M Tris-HCl,pH6.8 | 1L | ||
abs9201 | 1M Tris-HCl,pH8.8 | 1L | ||
abs9117 | SDS | 100ml | ||
★★ | abs9116 | WB/IP 細胞裂解液 | 100ml | |
★★ | abs9239 | WB/IP 細胞裂解液(無抑制劑) | 100ml | |
★★★★ | abs9101 | 紅細胞裂解液 | 100ml,250ml,500ml | |
abs812852 | PMSF | 50mg,100mg,200mg | ||
abs816442 | Aprotinin 抑肽酶 | 10mg,25mg,50mg,100mg | ||
abs817902 | Peptatin 胃蛋白酶抑制劑A | 10mg,25mg,50mg,100mg | ||
abs9133 | EDTA | 100ml | ||
abs42017684 | EGTA | 1g,10g | ||
abs817431 | 原釩酸鹽 | 100mg,500mg | ||
abs47014865 | β-甘油磷酸鹽 | 10g,25g | ||
★★ | abs9161 | 廣譜型蛋白酶抑制劑混合物 | 1ml | |
★★ | abs9162 | 廣譜型磷酸酶抑制劑混合物 | 1ml | |
蛋白定量 | ★★ | abs9232 | BCA蛋白定量試劑盒 | 500次,2500次 |
★★ | abs9233 | 增強型BCA 蛋白定量試劑盒 | 500次 | |
上樣與電泳 | abs9226 | DTT溶液(1mol/L) | 5ml,10ml | |
abs9237 | 2X Laemmli樣品緩沖液 | 5ml*2 | ||
abs9238 | 10%SDS十二烷基硫酸鈉溶液 | 500ml | ||
★★ | abs953 | 2*上樣緩沖液 (適用于SDS-PAGE) | 10ml | |
★★ | abs9301 | 預(yù)制膠 10%,10 wells | 5片/盒 | |
★★ | abs9308 | 預(yù)制膠 10%,15 wells | 5片/盒 | |
★★ | abs9302 | 預(yù)制膠 12%,10 wells | 5片/盒 | |
★★ | abs9309 | 預(yù)制膠 12%,15 wells | 5片/盒 | |
abs9303 | 預(yù)制膠 15% ,10 wells | 5片/盒 | ||
abs9310 | 預(yù)制膠 15% ,15 wells | 5片/盒 | ||
abs9304 | 預(yù)制膠 4-15%, 10 wells | 5片/盒 | ||
abs9311 | 預(yù)制膠 4-15%, 15 wells | 5片/盒 | ||
★★ | abs9305 | 預(yù)制膠 4-20%, 10 wells | 5片/盒 | |
★★ | abs9312 | 預(yù)制膠 4-20%, 15 wells | 5片/盒 | |
abs9307 | 預(yù)制膠 8%, 15 wells | 5片/盒 | ||
abs9306 | 預(yù)制膠 8-20%, 10 wells | 5片/盒 | ||
abs9313 | 預(yù)制膠 8-20%, 15 wells | 5片/盒 | ||
NEW | abs9328 | 3-5kd小分子量預(yù)制膠,16.5%,10wells | 10片/盒 | |
NEW | abs9329 | 3-5kd小分子量預(yù)制膠,16.5%,15wells | 10片/盒 | |
★★★ | abs925 | 蛋白電泳套裝 | 盒 | |
abs951 | running buffer | 100ml,1L | ||
★★★★★ | abs923 | 預(yù)染蛋白marker, 10-245 KDa | 500ul,5*500ul | |
★★★★★ | abs924 | 預(yù)染蛋白marker, 10-180 kDa | 250ul,2*250ul | |
abs966 | Genshare考馬斯亮藍蛋白膠快速染色液 | 500ml | ||
★★★ | abs964 | 考馬斯亮藍染色試劑盒(常規(guī)型)R250 | 1kit | |
abs965 | 考馬斯亮藍染色試劑盒(增強型)G250 | 1kit | ||
轉(zhuǎn)膜 | abs931 | PVDF膜0.2 μm | 20張 | |
★★ | abs932 | PVDF膜0.45 μm | 20張 | |
★★ | abs959 | NC膜0.2 μm | 20張 | |
abs960 | NC膜0.45 μm | 20張 | ||
abs950 | 10*電轉(zhuǎn)液 | 100ml,1L | ||
封閉 | abs9174 | 脫脂奶粉 | 100g,500g | |
★★★★★ | abs9157 | BSA | 50g,100g | |
一抗孵育 | ★★ | abs830031 | Beta actin antibody | 50ul,100ul |
abs132001 | Beta actin antibody | 50ug,100ug | ||
abs119680 | Beta Actin Polyclonal Antibody | 50ul,100ul | ||
abs132184 | Beta-Actin antibody | 50ug,100ug | ||
abs137975 | Beta-Actin Mouse Monoclonal Antibody | 50ug,100ug | ||
★★ | abs830030 | GAPDH antibody | 50ul,100ul | |
abs132004 | GAPDH antibody | 50ug,100ug | ||
abs137959 | GAPDH Mouse Monoclonal Antibody | 50ug,100ug | ||
abs118936 | GAPDH Polyclonal Antibody | 50ul,100ul | ||
abs131993 | Tubulin alpha Antibody | 50ug,100ug | ||
★★ | abs830032 | Tubulin Beta antibody | 50ul,100ul | |
abs131994 | Tubulin beta Antibody | 50ug,100ug | ||
abs125209 | Tubulin Beta Polyclonal Antibody | 50ul,100ul | ||
二抗孵育 | ★★ | abs928 | Goat anti-mouse IgG-HRP | 100ul,500ul,1ml |
★★ | abs20002 | Goat anti-rabbit IgG-HRP | 100ul,500ul,1ml | |
★★ | abs20003 | Goat anti-Mouse IgG-FITC | 1ml,500ul,100ul | |
★★ | abs20004 | Goat anti-Rabbit IgG-FITC | 100ul,500ul,1ml | |
★★ | abs20005 | Rabbit anti-Goat IgG-HRP | 100ul,500ul,1ml | |
一抗孵育 | abs830001 | Anti- GST | 50ul,100ul | |
abs830002 | Anti- His Tag抗體 | 50ul,100ul | ||
abs830003 | Anti- His(C-terminal Specific) 抗體 | 50ul,100ul | ||
abs830004 | Anti- Myc 抗體 | 50ul,100ul | ||
abs830005 | Anti- DYKDDDDK | 50ul,100ul | ||
abs830006 | Anti- HA 抗體 | 50ul,100ul | ||
abs830008 | Anti-V5,mAb | 50ul,100ul | ||
abs830010 | Anti- GST,HRP | 50ul | ||
abs830011 | Anti- His Tag (HRP) 抗體 | 50ul | ||
abs830012 | Anti- His(C-terminal Specific) (HRP) 抗體 | 50ul | ||
abs830013 | Anti- Myc (HRP) 抗體 | 50ul | ||
abs830014 | Anti- DYKDDDDK,HRP | 50ul | ||
abs830015 | Anti- HA (HRP) 抗體 | 50ul | ||
abs830016 | Anti- V5 (HRP) 抗體 | 50ul | ||
abs137956 | C-Myc-Tag Mouse Monoclonal Antibody | 50ug,100ug | ||
abs137958 | Flag-Tag Mouse Monoclonal Antibody | 50ug,100ug | ||
顯色 | ★★★★★ | abs920 | ECL化學(xué)發(fā)光檢測試劑盒 | 2*25ml,2*50ml,2*250ml |
abs9212 | DAB 顯色試劑盒(棕黃色,WB) | 1kit | ||
abs9213 | DAB 顯色試劑盒(紫藍色,WB) | 1kit | ||
其他輔助試劑 | abs952 | 10*TBST | 100ml,1L | |
abs9152 | Tween-20 | 500ml | ||
★★ | abs954 | WB一抗二抗稀釋液 | 100ml | |
abs969 | Western一抗二抗去除液(中性) | 250ml | ||
abs968 | Western一抗二抗去除液(弱堿性) | 250ml | ||
abs967 | Western一抗二抗去除液(強堿性) | 250ml | ||
新手* | ★★★★★ | abs926 | WB solution base kit(rabbit) | 1kit |
★★★★★ | abs927 | WB solution base kit(mouse) | 1kit | |
★★★★★ | abs928 | WB solution base kit(goat) | 1kit |
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