Rubicon RB4490/R400490 ThruPLEX Plasma-seq 12S Kit 血漿游離DNA測(cè)序試劑盒紅榮微再*代理基因測(cè)序試劑盒

美國(guó)Rubicon Genomics---單細(xì)胞測(cè)序文庫(kù)構(gòu)建的

1. 二代測(cè)序建庫(kù)試劑（微量起始樣品 50pg - 50ng）

2. 單細(xì)胞擴(kuò)增和單細(xì)胞測(cè)序建庫(kù)試劑（廣泛應(yīng)用于生殖領(lǐng)域，是該領(lǐng)域的金標(biāo)準(zhǔn)。）

3. 全RNA擴(kuò)增試劑 （微量起始樣品，可以低至10個(gè)細(xì)胞的總RNA）

Rubicon Genomics成立于2000年，總部位于美國(guó)密歇根州。該公司的核心競(jìng)爭(zhēng)力產(chǎn)品包括酶學(xué)、核酸化學(xué)、樣品的加工生產(chǎn)，該公司致力于創(chuàng)新、知識(shí)產(chǎn)權(quán)的發(fā)展和持續(xù)的產(chǎn)品開發(fā)。

Rubicon Genomics開發(fā)創(chuàng)新、高品質(zhì)的、核酸文庫(kù)的制備及配套產(chǎn)品，血漿游離DNA基因測(cè)序試劑盒能夠?qū)εR床樣本進(jìn)行簡(jiǎn)單、快速可靠和高靈敏度的分析。

Rubicon Genomics，總部位于美國(guó)密歇根州，產(chǎn)品主要包括以下幾類：

【血漿游離DNA基因測(cè)序試劑盒主要產(chǎn)品】

1. ThruPLEX® Plasma-seq Kit

 2. ThruPLEX® DNA-seq Kit

 3. PicoPLEX® DNA-seq Kit

 4. PicoPLEX® WGA Kit

 5. TransPLEX® C-WTA Kit

【血漿游離DNA基因測(cè)序試劑盒產(chǎn)品應(yīng)用】

 1. 外顯子組富集

 2. SureSelect® 富集

 3. SeqCap® EZ 富集

 4. 福爾馬林固定石蠟包埋DNA測(cè)序

 5. 胚胎植入前遺傳學(xué)篩查(PGS）/種植前遺傳學(xué)診斷（PGD）

 6. DNA測(cè)序

 7. RNA測(cè)序

 8. 染色體免疫共沉淀測(cè)序

【產(chǎn)品詳情】

歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348

提供產(chǎn)品：華雅再生醫(yī)學(xué)旗艦公司-紅榮微再（上海）生物工程技術(shù)有限公司為中國(guó)再生醫(yī)學(xué)和生物工程技術(shù)領(lǐng)域的組織器官再生研究、組織器官功能重建研究、干細(xì)胞技術(shù)研究、組織工程生物支架材料研究、器官移植醫(yī)療器械產(chǎn)品等再生醫(yī)學(xué)產(chǎn)業(yè)鏈的相關(guān)客戶提供：iPS/ES胚胎干細(xì)胞的種子細(xì)胞、心肌細(xì)胞、人源肝臟細(xì)胞、MSC間充質(zhì)干細(xì)胞、基底膜基質(zhì)膠細(xì)胞支架、干細(xì)胞生長(zhǎng)分化調(diào)控因子、干細(xì)胞藥物研究的小分子抑制劑、調(diào)節(jié)劑、小分子化合物、功能膠原蛋白生物材料、3D細(xì)胞培養(yǎng)基質(zhì)膠、器官模型3D打印技術(shù)材料、神經(jīng)干細(xì)胞培養(yǎng)基、STEM121抗體、BD科研P100、P800蛋白質(zhì)組學(xué)采血管、BD血漿準(zhǔn)備管PPT管、BD PAXgene全血RNA樣本收集管、BD公司CPT/PRP美容采血管、Streck Cell-Free RNA BCT游離RNA樣本收集管、Streck Cell-Free DNA Urine Preserve游離DNA尿液保存管、Streck Cell-Free DNA BCT游離DNA采血管、循環(huán)腫瘤細(xì)胞CTC及*產(chǎn)前基因檢測(cè)采血管、illumina高品質(zhì)NGS文庫(kù)制備試劑盒、ThruPLEX ctDNA測(cè)序試劑盒、全基因組測(cè)序、全外顯子測(cè)序、靶向測(cè)序、ChIP-Seq測(cè)序、CNV和染色體異倍性分析、Rubicon Genomics單細(xì)胞全基因組測(cè)序試劑盒、生物醫(yī)學(xué)試劑耗材儀器等種類豐富的產(chǎn)品，以“Focus on Tissue and Organ Regeneration專注組織器官再生與功能重建”為己任，我們與客戶攜手為中國(guó)再生醫(yī)學(xué)產(chǎn)業(yè)的發(fā)展而前行。

Overview

ThruPLEX® Plasma-seq is powered by ThruPLEX chemistry to generate high performance NGS libraries from cell-free DNA isolated from plasma.

The chemistry is optimized specifically for cell-free DNA to maximize the library complexity and to preserve the GC representation of the input DNA. Libraries can be used for CNV analysis, whole genome sequencing applications or enriched using your custom panels or leading target enrichment platforms, such as Agilent SureSelect® and Roche NimbleGen SeqCap® EZ.

Libraries prepared with ThruPLEX Plasma-seq provide added sensitivity to better understand genetic variations found within the plasma samples. Plasma, along with other liquid biopsies, has gained significant interest in the research community as a valuable and easily available sample type.  These communities include translational research in oncology and developmental research involving the fetal fraction derived from maternal plasma.

ThruPLEX® Plasma-seq Kit is intended for Research Use Only. It may not be used for any other purposes including, but not limited to, use in diagnostics, forensics, therapeutics, or in humans. ThruPLEX Plasma-seq may not be transferred to third parties, resold, modified for resale or used to manufacture commercial products without prior written approval of Rubicon Genomics, Inc.ThruPLEX Plasma-seq Kit  is protected by U.S. Patents 7,803,550; 8,071,312; 8,399,199; 8,728,737 and corresponding foreign patents. Additional patents are pending.

Designed for cell-free DNA: newly formulated repair and ligation reagents with optimized protocols

High performance NGS libraries: high diversity with broad and reproducible GC coverage

Variable sample input: <1 ng to 30 ng of cell-free DNA

Fast and simple workflow: 3 steps in a single tube or well in 2 hours with no purification or sample transfer steps

Research applications: libraries from samples where results require high sensitivity including liquid biopsies, ctDNA, and targeted sequencing

Automation-friendly: Beckman® FXP Workstation

Highest Diversity, Fewest Unmapped Reads from Cell-Free DNA

ThruPLEX Plasma-seq Kit generated quality libraries with high diversity and a low number of duplicates and unmapped reads. Cell-free DNA was extracted from 3 plasma samples, and libraries were prepared at the amounts indicated as measured by Qubit廬. The amount of mononucleosomal DNA in each sample as measured by the Bioanalyzer廬 corresponded to 0.09 ng, 0.62 ng, and 15.44 ng. Pooled libraries were sequenced on an Illumina NextSeq廬 500 as a paired-end run with 17M to 25M reads per library, Duplication rates were calculated after down-sampling the data to 17 M reads per library.

歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348

提供產(chǎn)品：華雅再生醫(yī)學(xué)旗艦公司-紅榮微再（上海）生物工程技術(shù)有限公司為中國(guó)再生醫(yī)學(xué)和生物工程技術(shù)領(lǐng)域的組織器官再生研究、組織器官功能重建研究、干細(xì)胞技術(shù)研究、組織工程生物支架材料研究、器官移植醫(yī)療器械產(chǎn)品等再生醫(yī)學(xué)產(chǎn)業(yè)鏈的相關(guān)客戶提供：iPS/ES胚胎干細(xì)胞的種子細(xì)胞、心肌細(xì)胞、人源肝臟細(xì)胞、MSC間充質(zhì)干細(xì)胞、基底膜基質(zhì)膠細(xì)胞支架、干細(xì)胞生長(zhǎng)分化調(diào)控因子、干細(xì)胞藥物研究的小分子抑制劑、調(diào)節(jié)劑、小分子化合物、功能膠原蛋白生物材料、3D細(xì)胞培養(yǎng)基質(zhì)膠、器官模型3D打印技術(shù)材料、神經(jīng)干細(xì)胞培養(yǎng)基、STEM121抗體、BD科研P100、P800蛋白質(zhì)組學(xué)采血管、BD血漿準(zhǔn)備管PPT管、BD PAXgene全血RNA樣本收集管、BD公司CPT/PRP美容采血管、Streck Cell-Free RNA BCT游離RNA樣本收集管、Streck Cell-Free DNA Urine Preserve游離DNA尿液保存管、Streck Cell-Free DNA BCT游離DNA采血管、循環(huán)腫瘤細(xì)胞CTC及*產(chǎn)前基因檢測(cè)采血管、illumina高品質(zhì)NGS文庫(kù)制備試劑盒、ThruPLEX ctDNA測(cè)序試劑盒、全基因組測(cè)序、全外顯子測(cè)序、靶向測(cè)序、ChIP-Seq測(cè)序、CNV和染色體異倍性分析、Rubicon Genomics單細(xì)胞全基因組測(cè)序試劑盒、生物醫(yī)學(xué)試劑耗材儀器等種類豐富的產(chǎn)品，以“Focus on Tissue and Organ Regeneration專注組織器官再生與功能重建”為己任，我們與客戶攜手為中國(guó)再生醫(yī)學(xué)產(chǎn)業(yè)的發(fā)展而前行。

Outstanding Target Enrichment Peformance

ThruPLEX Plasma-seq Kit libraries were captured at high efficiency and generated data with deep coverage of the kinome for mutation detection. Libraries were prepared from 3 plasma samples at input amounts of 5 ng, 6.5 ng, and 10 ng in triplicate, and targeted sequencing was carried out on an Illumina MiSeq using samples enriched with the ClearSeq Human DNA Kinome Panel for SureSelectXT2. On average, 5M reads were generated per library. Selected bases were successfully captured bases that were in or within 250 bp of the baits.

Reproducible, Unbiased GC Coverage

ThruPLEX Plasma-seq Kit provided the most reproducible and unbiased GC coverage across the human genome. ThruPLEX libraries showed minimal variability across 9 individual plasma samples tested. Libraries were prepared from cell-free DNA isolated from 1 mL of plasma samples and sequenced on an Illumina NextSeq 500. Four separate plasma samples were used to construct the NEBNext Ultra libraries.

Evaluated at Karolinska Institute and CRUK

Illumina NGS libraries were prepared with 5 ng of cell-free DNA isolated from pooled plasma samples using ThruPLEX Plasma-seq Kit. Low-pass WGS was conducted on a HiSeq with approximay 12.5M reads per library. (Data courtesy of the Karolinska Institute, Sweden).

ThruPLEX Plasma-seq Kit Single-Tube Workflow

Starting with 1 to 30 ng of cell-free DNA, ThruPLEX Plasma-seq Kit creates indexed libraries in 3 simple steps: end repair, adapter ligation, and high-fidelity library amplification. No purification or sample transfer steps are required. The streamlined workflow is performed in 2 hours in a single tube or well, preventing sample loss and enhancing positive sample identification.

ThruPLEX Plasma-seq Kit Technology

ThruPLEX Plasma-seq Kit technology is a 3-step reaction that is optimized for cell-free DNA. Cell-free DNA (1 ng to 30 ng) is first repaired in a highly efficient process. Background is reduced using double-stranded adapters with no single-stranded tails. Blunt-end ligation occurs with high-efficiency. Blocked 5’ ends reduce adapter-adapter ligation. Background is further reduced by destroying unused adapters after ligation. A high-fidelity amplification completes the reaction to generate indexed Illumina libraries.

歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348

提供產(chǎn)品：華雅再生醫(yī)學(xué)旗艦公司-紅榮微再（上海）生物工程技術(shù)有限公司為中國(guó)再生醫(yī)學(xué)和生物工程技術(shù)領(lǐng)域的組織器官再生研究、組織器官功能重建研究、干細(xì)胞技術(shù)研究、組織工程生物支架材料研究、器官移植醫(yī)療器械產(chǎn)品等再生醫(yī)學(xué)產(chǎn)業(yè)鏈的相關(guān)客戶提供：iPS/ES胚胎干細(xì)胞的種子細(xì)胞、心肌細(xì)胞、人源肝臟細(xì)胞、MSC間充質(zhì)干細(xì)胞、基底膜基質(zhì)膠細(xì)胞支架、干細(xì)胞生長(zhǎng)分化調(diào)控因子、干細(xì)胞藥物研究的小分子抑制劑、調(diào)節(jié)劑、小分子化合物、功能膠原蛋白生物材料、3D細(xì)胞培養(yǎng)基質(zhì)膠、器官模型3D打印技術(shù)材料、神經(jīng)干細(xì)胞培養(yǎng)基、STEM121抗體、BD科研P100、P800蛋白質(zhì)組學(xué)采血管、BD血漿準(zhǔn)備管PPT管、BD PAXgene全血RNA樣本收集管、BD公司CPT/PRP美容采血管、Streck Cell-Free RNA BCT游離RNA樣本收集管、Streck Cell-Free DNA Urine Preserve游離DNA尿液保存管、Streck Cell-Free DNA BCT游離DNA采血管、循環(huán)腫瘤細(xì)胞CTC及*產(chǎn)前基因檢測(cè)采血管、illumina高品質(zhì)NGS文庫(kù)制備試劑盒、ThruPLEX ctDNA測(cè)序試劑盒、全基因組測(cè)序、全外顯子測(cè)序、靶向測(cè)序、ChIP-Seq測(cè)序、CNV和染色體異倍性分析、Rubicon Genomics單細(xì)胞全基因組測(cè)序試劑盒、生物醫(yī)學(xué)試劑耗材儀器等種類豐富的產(chǎn)品，以“Focus on Tissue and Organ Regeneration專注組織器官再生與功能重建”為己任，我們與客戶攜手為中國(guó)再生醫(yī)學(xué)產(chǎn)業(yè)的發(fā)展而前行。

2017年1月，Rubicon Genomics，Inc.成為Takara Bio的一員，現(xiàn)在寶日醫(yī)生物技術(shù)（北京）有限公司（Takara Bio北京分公司）開始在國(guó)內(nèi)銷售Rubicon公司開發(fā)的下一代測(cè)序試劑。Rubicon公司成立于2000年，公司總部位于美國(guó)密歇根州。Rubicon公司開發(fā)的產(chǎn)品主要基于“ThruPLEX®技術(shù)”和“PicoPLEX®技術(shù)”，可以微量DNA起始構(gòu)建下一代測(cè)序文庫(kù)。ThruPLEX®技術(shù)”可從血漿中的微量游離DNA構(gòu)建高多樣性文庫(kù)，準(zhǔn)確檢測(cè)低豐度DNA變異，用途廣泛。“PicoPLEX®技術(shù)”，可從單細(xì)胞等微量DNA起始構(gòu)建高重復(fù)再現(xiàn)性文庫(kù)，用于拷貝數(shù)變異分析和染色體異倍性分析。Rubicon產(chǎn)品豐富了Takara基于SMART®技術(shù)的下一代測(cè)序產(chǎn)品線，為科研工作者在基因組學(xué)、單細(xì)胞和臨床應(yīng)用研究等領(lǐng)域提供更多解決方案。代表產(chǎn)品之一為：Rubicon Genomics RB3050/R30050 PicoPLEX® WGA Kit 單細(xì)胞全基因組擴(kuò)增試劑盒Whole Genome Amplification from Single Cells。

2016年年底，TAKARA寶日醫(yī)生物技術(shù)（北京）有限公司的市場(chǎng)部部長(zhǎng)與寶生物工程（大連）有限公司的銷售部部長(zhǎng)分別從北京和大連飛往上海，對(duì)華雅再生醫(yī)學(xué)的旗艦營(yíng)銷公司：紅榮微再（上海）生物工程技術(shù)有限公司進(jìn)行了實(shí)地考察，雙方深入地探討了未來(lái)干細(xì)胞及再生醫(yī)學(xué)市場(chǎng)發(fā)展的趨勢(shì)和方向，在雙方公司中長(zhǎng)期發(fā)展戰(zhàn)略上，志同道合地看好再生醫(yī)學(xué)市場(chǎng)的發(fā)展前景，歷史性的醫(yī)療革命是我們這代人肩負(fù)的偉大使命！

TAKARA公司日本總部是：“iPS干細(xì)胞之父”諾貝爾獎(jiǎng)獲得者的故鄉(xiāng)，具有zui前沿的iPS干細(xì)胞技術(shù)實(shí)力，TAKARA公司旗下的Cellartis子公司，擁有超過(guò)40人的研發(fā)團(tuán)隊(duì)，擁有超過(guò)15年的人干細(xì)胞研究經(jīng)驗(yàn)，建立并儲(chǔ)存大量的人iPS和ES細(xì)胞系。TAKARA旗下的Stem Cells公司SC Proven系列：憑借20多年干細(xì)胞技術(shù)經(jīng)驗(yàn)研發(fā)，包括干細(xì)胞研究用培養(yǎng)基、抗體等耗材，具有龐大的客戶群(美國(guó)、歐洲、日本)。

2017年TAKARA寶生物日本總部又收購(gòu)了：美國(guó)Rubicon Genomics單細(xì)胞測(cè)序文庫(kù)構(gòu)建的，代表性產(chǎn)品之一為：Rubicon Genomics RB3050/R30050 PicoPLEX® WGA Kit 單細(xì)胞全基因組擴(kuò)增試劑盒Whole Genome Amplification from Single Cells，ThruPLEX® Plasma-seq Kit，ThruPLEX® DNA-seq Kit，PicoPLEX® DNA-seq Kit，PicoPLEX® WGA Kit， TransPLEX® C-WTA Kit.

常見問題解答

• ThruPLEX Plasma-seq可以使用什么類型的樣品和輸入？

ThruPLEX Plasma-seq Kit旨在從分離自血漿的無(wú)細(xì)胞DNA中產(chǎn)生Illumina NGS的DNA文庫(kù)。 ThruPLEX Plasma-seq化學(xué)被重新制備，以產(chǎn)生具有廣泛GC覆蓋率的多種文庫(kù)，提供1至30ng *無(wú)細(xì)胞DNA的可重復(fù)測(cè)序結(jié)果。

應(yīng)用輸入樣品類型推薦輸入量

血漿和液體活組織檢查DNA測(cè)序; ctDNA分析，靶向測(cè)序從血漿中分離的無(wú)細(xì)胞DNA 1 ng - 30 ng *

庫(kù)的輸入量小于1 ng，超過(guò)30 ng。

• ThruPLEX DNA-seq Kit和新型ThruPLEX Plasma-seq Kit之間的主要技術(shù)差異是什么？

ThruPLEX Plasma-seq Kit已經(jīng)針對(duì)從血漿中分離的無(wú)細(xì)胞DNA進(jìn)行了優(yōu)化。

ThruPLEX Plasma-seq Kit不需要剪切或破碎無(wú)細(xì)胞DNA。

協(xié)議是不同的（見下文）。

• ThruPLEX Plasma-seq和ThruPLEX DNA-seq之間的協(xié)議差異是甚么？

模板制備步驟：體積和保持溫度

庫(kù)的合成步驟：體積和培養(yǎng)溫度

文庫(kù)擴(kuò)增步驟：擴(kuò)增循環(huán)的體積和數(shù)量

• 來(lái)自ThruPLEX Plasma-seq的試劑可以與ThruPLEX DNA-seq？

不，ThruPLEX Plasma-seq Kit的內(nèi)容物與其他Rubicon Genomics產(chǎn)品不可互換。

• 你有什么類型的索引或條形碼？

ThruPLEX Plasma-seq 12S和48S試劑盒提供分布在管或96孔板中的與Illumina兼容的8nt單指數(shù)。這些索引序列由英國(guó)劍橋的Wellcome Trust Sanger研究所開發(fā)。關(guān)于Sanger指數(shù)序列的信息可以在Nature Methods 7,111-118（2010）中找到。

ThruPLEX Plasma-seq 96D試劑盒配備了分布在96孔板中的Illumina TruSeq HT i5和i7雙重指標(biāo)。 i5索引具有Illumina D501至D508序列，i7索引具有D701至D712序列。

有關(guān)索引的更多信息，請(qǐng)參閱“ThruPLEX Plasma-seq套件使用手冊(cè)”中的附錄1。

• 您有什么建議將血漿中的無(wú)細(xì)胞DNA分離出來(lái)？

使用QIAamp®循環(huán)核酸試劑盒（Qiagen，CAT.NO.55114）制備的無(wú)細(xì)胞DNA優(yōu)化ThruPLEX Plasma-seq。

含有輸入DNA的緩沖液中Tris和EDTA的濃度分別不得超過(guò)10mM和0.1mM。避免含磷酸鹽緩沖液。

• 我應(yīng)該使用什么樣的收集管來(lái)吸取受試者的血液？

Rubicon Genomics的科學(xué)人員使用K2 EDTA（Becton Dickinson，CAT。NO。36643）在Vacutainer Venous Blood Collection Tubes中收集的血液。

• 在分離無(wú)細(xì)胞DNA之前，我應(yīng)該如何在血液中制備血漿？

使用雙離心方案將血漿與血液分離。將處理過(guò)的血漿樣品在-80°處儲(chǔ)存，直到不分離DNA。

• 從ThruPLEX Plasma-seq Kit中可以使用從尿液或其他生物流體中分離的無(wú)細(xì)胞DNA嗎？

ThruPLEX Plasma-seq用從人血漿中分離的無(wú)細(xì)胞DNA進(jìn)行了優(yōu)化;然而，該試劑盒可能與來(lái)自其他生物流體的降解的DNA一起使用。必須首先用DNA提取/純化試劑盒提取其他樣品來(lái)源的無(wú)細(xì)胞DNA。由于來(lái)自這些來(lái)源的無(wú)細(xì)胞DNA通常被降解/分裂，因此不需要額外的碎裂。每個(gè)反應(yīng)的無(wú)細(xì)胞輸入DNA的體積不得超過(guò)10μL。

• ThruPLEX Plasma-seq Kit是否與任何目標(biāo)濃縮系統(tǒng)兼容？

是的，ThruPLEX Plasma-seq Kit與主要的exome和目標(biāo)富集產(chǎn)品兼容，包括AgilentSureSelect®，RocheNimbleGen®SeqCap®EZ和定制面板。

紅榮微再（上海）生物工程技術(shù)有限公司主營(yíng)：干細(xì)胞、精準(zhǔn)醫(yī)療、細(xì)胞治療、器官再生 四大板塊的產(chǎn)品，以“傳遞科學(xué)價(jià)值，服務(wù)科學(xué)研究”為宗旨，經(jīng)過(guò)近兩年的努力推廣，紅榮微再（上海）生物工程技術(shù)有限公司在全中國(guó)代理的CellArtis干細(xì)胞產(chǎn)品已經(jīng)成功銷往：上海市、北京市、重慶市、天津市、廣東省的廣州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、綿陽(yáng)、浙江省的杭州市、寧波、溫州、江蘇省的南京市、蘇州、無(wú)錫、泰州、湖南省的長(zhǎng)沙、陜西省的西安市、楊凌、甘肅省的蘭州、寧夏的銀川、新疆的烏魯木齊、廣西省的南寧市、山東省的濟(jì)南、青島、東北三省 遼寧省的沈陽(yáng)市、大連市、吉林省的長(zhǎng)春市、黑龍江省的哈爾濱市等中國(guó)絕大部分省市和自治區(qū)。

歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348

FAQS

1. What types of samples and inputs can be used with ThruPLEX Plasma-seq?

ThruPLEX Plasma-seq Kit is designed to generate DNA libraries for Illumina NGS from cell-free DNA isolated from plasma. The ThruPLEX Plasma-seq chemistry is reformulated to produce diverse libraries with broad GC coverage, providing reproducible sequencing results from 1 to 30 ng* of cell-free DNA.

Application Input sample type Recommended input amount

Sequencing of DNA from Plasma & Liquid Biopsy; ctDNA Analysis, Targeted Sequencing Cell-free DNA isolated from plasma 1 ng – 30 ng*

*Libraries have been generated from input amounts less than 1 ng and over 30 ng.

Contact technical support for further information.

2. What are the major technical differences between ThruPLEX DNA-seq Kit and the new ThruPLEX Plasma-seq Kit?

ThruPLEX Plasma-seq Kit has been optimized for cell-free DNA isolated from plasma.

Shearing or fragmentation of the cell-free DNA is not necessary with ThruPLEX Plasma-seq Kit.

The protocol is different (see below).

3.  What are the protocol differences between the ThruPLEX Plasma-seq and ThruPLEX DNA-seq?

Template Preparation Step: volumes and holding temperature

Library Synthesis Step: volumes and incubation temperature

Library Amplification Step: volumes and number of amplification cycles

4.  Can the reagents from ThruPLEX Plasma-seq be interchanged with the reagents from ThruPLEX DNA-seq?

No, contents of ThruPLEX Plasma-seq Kit are not interchangeable with other Rubicon Genomics products.

5.  What type of indexes or barcodes do you have available?

ThruPLEX Plasma-seq 12S and 48S Kits are provided with Illumina-compatible 8nt single indexes dispensed in tubes or a 96-well plate. These index sequences were developed by the Wellcome Trust Sanger Institute in Cambridge, UK. Information about the Sanger index sequences can be found in Nature Methods 7, 111-118 (2010).

ThruPLEX Plasma-seq 96D Kit are provided with the Illumina TruSeq HT i5 and i7 dual indexes dispensed in a 96-well plate. The i5 indexes have the Illumina D501 to D508 sequences, and the i7 indexes have the D701 to D712 sequences.

For more information about the indexes, refer to Appendix 1 in the ThruPLEX Plasma-seq Kit Instruction Manual.

紅榮微再（上海）生物工程技術(shù)有限公司主營(yíng)：干細(xì)胞、精準(zhǔn)醫(yī)療、細(xì)胞治療、器官再生 四大板塊的產(chǎn)品，以“傳遞科學(xué)價(jià)值，服務(wù)科學(xué)研究”為宗旨，經(jīng)過(guò)近兩年的努力推廣，紅榮微再（上海）生物工程技術(shù)有限公司在全中國(guó)代理的CellArtis干細(xì)胞產(chǎn)品已經(jīng)成功銷往：上海市、北京市、重慶市、天津市、廣東省的廣州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、綿陽(yáng)、浙江省的杭州市、寧波、溫州、江蘇省的南京市、蘇州、無(wú)錫、泰州、湖南省的長(zhǎng)沙、陜西省的西安市、楊凌、甘肅省的蘭州、寧夏的銀川、新疆的烏魯木齊、廣西省的南寧市、山東省的濟(jì)南、青島、東北三省 遼寧省的沈陽(yáng)市、大連市、吉林省的長(zhǎng)春市、黑龍江省的哈爾濱市等中國(guó)絕大部分省市和自治區(qū)。

歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348

6.  What recommendations do you have for isolating the cell-free DNA from plasma?

ThruPLEX Plasma-seq was optimized with cell-free DNA prepared using the QIAamp® Circulating Nucleic Acid Kit (Qiagen, CAT. NO. 55114).

The concentrations of Tris and EDTA in the buffer containing the input DNA must not exceed 10 mM and 0.1 mM, respectively. Avoid phosphate containing buffers.

7.  What kind of collection tube should I use to draw blood from subjects?

The scientific staff at Rubicon Genomics uses blood collected in Vacutainer Venous blood Collection Tubes with K2 EDTA (Becton Dickinson, CAT. NO. 36643).

For detailed instructions, refer to the Blood Collection and Plasma Preparation Protocol at the ThruPLEX Plasma-seq Product Page, under the Resources tab:  http://rubicongenomics.com/products/thruplex-plasma-seq-kit/.

8.  How should I prepare plasma from blood before isolating the cell-free DNA?

Plasma should be separated from blood using a double-centrifugation protocol. Store processed plasma samples at –80° until cell-free DNA is to be isolated.

For detailed instructions, refer to the Blood Collection and Plasma Preparation Protocol at the ThruPLEX Plasma-seq Product Page, under the Resources tab:  http://rubicongenomics.com/products/thruplex-plasma-seq-kit/.

9.  Can cell-free DNA isolated from urine or other biofluids be used with ThruPLEX Plasma-seq Kit?

ThruPLEX Plasma-seq was optimized with cell-free DNA isolated from human plasma; however, the kit may work with degraded DNA from other biofluids. Cell-free DNA from other sample sources must first be extracted with a DNA extraction/purification kit. Since cell-free DNA from these sources is normally degraded/fragmented, additional fragmentation is unnecessary. The volume of input cell-free DNA for each reaction must not exceed 10 µL.

 10.  Is ThruPLEX Plasma-seq Kit compatible with any target enrichment systems?

Yes, ThruPLEX Plasma-seq Kit is compatible with the major exome and target enrichment products, including Agilent SureSelect®, Roche NimbleGen® SeqCap® EZ and custom panels. ThruPLEX Plasma-seq Kit target enrichment protocols and application notes can be accessed at: http://rubicongenomics.com/applications/enrichment/.

紅榮微再（上海）生物工程技術(shù)有限公司主營(yíng)：干細(xì)胞、精準(zhǔn)醫(yī)療、細(xì)胞治療、器官再生 四大板塊的產(chǎn)品，以“傳遞科學(xué)價(jià)值，服務(wù)科學(xué)研究”為宗旨，經(jīng)過(guò)近兩年的努力推廣，紅榮微再（上海）生物工程技術(shù)有限公司在全中國(guó)代理的CellArtis干細(xì)胞產(chǎn)品已經(jīng)成功銷往：上海市、北京市、重慶市、天津市、廣東省的廣州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、綿陽(yáng)、浙江省的杭州市、寧波、溫州、江蘇省的南京市、蘇州、無(wú)錫、泰州、湖南省的長(zhǎng)沙、陜西省的西安市、楊凌、甘肅省的蘭州、寧夏的銀川、新疆的烏魯木齊、廣西省的南寧市、山東省的濟(jì)南、青島、東北三省 遼寧省的沈陽(yáng)市、大連市、吉林省的長(zhǎng)春市、黑龍江省的哈爾濱市等中國(guó)絕大部分省市和自治區(qū)。

歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：152 1681 4001。華雅再生醫(yī)學(xué)-客服: 316 808 6348