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全血RNA管和RNA保護液
閱讀：4605發(fā)布時間：2017-11-30
區(qū)別
PAXgene® Blood RNA Tube 全血RNA管
適用：血液樣本
Ambion® RNAlater™ Stabilization SolutionRNA 保護液、固定液
適用：組織樣品或液氮冷凍樣品
PAXgene® Blood RNA Tube 全血RNA管
產(chǎn)品英文名 PAXgene® Blood RNA Tube
產(chǎn)品中文名 PAXgene® 全血RNA管
產(chǎn)品介紹 PAXgene® 全血RNA管能穩(wěn)定血細胞內(nèi)的RNA水平，提供一份zui接近人體內(nèi)狀態(tài)的RNA標本，延長血液標本體外放置的時間，利于批量采集，細胞轉(zhuǎn)錄水平保真性好，可用于與血細胞RNA相關(guān)的RT-PCR檢測，人類疾病基因轉(zhuǎn)錄譜的研究及其他RNA轉(zhuǎn)錄相關(guān)的檢測。特殊RNA穩(wěn)定劑、zui小化體外血RNA降解或基因誘導，延長標本體外放置時間，全血RNA原管室溫保存3~5天，凍存條件下儲存8年。
產(chǎn)品訂購信息
品牌 | 貨號 | 產(chǎn)品描述 | 包裝 |
BD | 762165 | 2.5mL全血RNA管，6.9mL添加劑，紅色安全蓋 | 100支/箱 |
詳細說明
Tube Material Plastic
Tube Size 16x100 mm
Draw Volume 2.5 mL
Closure Type BD Hemogard™
Closure Color Red
Label Paper
Cell Separator No
Additive 6.9 mL of Proprietary RNA stabilization additive
Sterilization Radiation, 10-6 SAL, ISO11137-1
Regulatory Status IVD
Intended Use
The PAXgene® Blood RNA System consists of a blood collection tube (PAXgene® Blood RNA Tube) and nucleic acid purification kit (PAXgene® Blood RNA Kit). It is intended for the collection, storage, and transport of blood and stabilization of intracellular RNA in a closed tube and subsequent isolation and purification of intracellular RNA from whole blood for RT-PCR used in molecular diagnostic testing.
Performance characteristics for the PAXgene® Blood RNA System have only been established with FOS and IL1B gene transcripts. The user is responsible for establishing appropriate PAXgene® Blood RNA System performance haracteristics for other target transcripts.
Warnings
1. Contents of this tube are irritating to skin.
• After inhalation, supply fresh air: consult doctor in case of complaints.
• After skin contact, immediay wash with water and soap, and rinse thoroughly.
• After eye contact, rinse opened eye for fifteen minutes under running water, then consult a doctor.
• After swallowing, immediay call a doctor.
A blood collection set must be used with the PAXgene® Blood RNA Tube. See Ordering Information.
Limitations
1. Under-filling of the PAXgene® Blood RNA Tubes will result in an incorrect blood-to-additive
ratio and may lead to incorrect analytic results or poor product performance.
2. The PAXgene® Blood RNA System is not suitable for the collection and purification of viral
RNA.
3. Blood samples obtained with the PAXgene® Blood RNA Tube must be prepared only with the PAXgene® Blood RNA Kit. For catalog numbers, see Ordering Information.
4. The quantity of blood drawn should be 2.5mL per PAXgene® Blood RNA Tube, but this volume might vary with altitude, ambient temperature, barometric pressure, tube age, venous pressure, and filling technique.
5. The PAXgene® Blood RNA Kit is intended for purification of intracellular RNA from human whole blood with leukocytes counts between 4.8 x 106- 1.1 x 107leukocytes/mL.
Precautions
1. Practice universal precautions. Use gloves, gowns, eye protection, other personal protective equipment, and engineering controls to protect from blood splatter, blood leakage, and potential exposure to bloodborne pathogens.
2. Handle all biologic samples and blood collection sets "sharps" according to the policies and procedures of your facility. Obtain appropriate medical attention in the event of any exposure to biologic samples (for example,through a puncture injury), since they may transmit viral hepatitis, HIV (AIDS), or other infectious diseases.Utilize any built-in needle protector, if the blood collection set provides one. PreAnalytiX does not recommend reshielding used needles. However, the policies and procedures of your facility may differ and must always be followed.
3. Discard all blood collection tubes in biohazard containers approved for their disposal.
4. Do not re-use the PAXgene® Blood RNA Tubes.
5. Do not use the PAXgene® Blood RNA Tubes after the expiration date printed on the tube label.
6. Since the PAXgene® Blood RNA Tube contains a chemical additive, a blood collection set must be used for blood collection to prevent possible backflow from the tube. See Specimen Collection and Preparation for Analysis section.
7. Excessive centrifugation speed (over 10,000 RCF) may cause the PAXgene® Blood RNA Tube breakage, exposure to blood and possible injury.
8. Do not transfer a sample from a syringe to a tube.
Storage
Store the unused PAXgene® Blood RNA Tubes at 18ºC to 25ºC. Limited excursion temperatures up to 40ºC are permitted.
762165 現(xiàn)貨歡迎您致電 華雅再生醫(yī)學旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司 ：1500 1904 520。紅榮微再-客服: 2395557778 經(jīng)銷商專員
Ambion® RNAlater™ Stabilization Solution RNA保護液
詳細介紹
產(chǎn)品英文名 Ambion® RNAlater™ Stabilization Solution
產(chǎn)品中文名 Ambion® RNAlater™ RNA 固定液
產(chǎn)品介紹 Ambion® RNAlater™ Stabilization Solution: RNA固定液為水溶性、無組織毒性的保存試劑，可快速滲透到組織中，固定并保護細胞RNA。RNAlater® 固定并保護未破壞的未冷凍組織樣品中的細胞RNA，無需為后續(xù)處理而快速處理組織樣品或液氮冷凍樣品。組織塊可獲得并浸入RNAlater® 中進行保存，不影響后續(xù)RNA分離所獲得的RNA數(shù)量和質(zhì)量。RNAlater® 可加到細胞團甚至培養(yǎng)基中的細胞中，然后樣品即可冷凍或不冷凍保存。
Ambion® RNAlater™ Stabilization Solution訂購信息
品牌 | 貨號 | 產(chǎn)品描述 | 包裝 | 單價 |
Thermo | AM7020 | Ambion® RNAlater™ Stabilization Solution RNA 保護液、固定液 | 100 mL | 1422元 |
Thermo | AM7021 | Ambion® RNAlater™ Stabilization Solution RNA 保護液、固定液 | 500 mL | 4444元 |
RNAlater™ Stabilization Solution RNA 保護液特點
• 一種試劑即可迅速滅活RNA酶，穩(wěn)定組織或細胞中的RNA
• 無需液氮冷凍樣品或?qū)悠房焖俎D(zhuǎn)入實驗室冰箱中
• 多數(shù)組織樣品無需冷凍和研磨
• “野外”組織采集的理想選擇
• RNA在 37°C 下可穩(wěn)定1天, 25°C 下1周, 4°C 下1個月, -20°C 下無限期。
• 與包括大多數(shù)RNA分離試劑盒在內(nèi)的多數(shù)RNA分離程序相兼容
RNA保護液上下游產(chǎn)品
品牌 | 貨號 | 產(chǎn)品描述 | 包裝 |
Thermo | 4444436 | TaqMan Fast Virus 1-Step Master Mix 酶 | 10mL/瓶 |
Thermo | AM9920 | DEPC-Treated Water DEPC處理水 | 500mL/瓶 |
Thermo | 15567027 | UltraPure 1M pH7.5 Tris-HCI Buffer緩沖液 | 1 L/瓶 |
Thermo | AM9262 | EDTA (0.5 M), pH 8.0 溶液 | 1000mL/瓶 |
Thermo | AM2222 | Ambion DNase I (RNase-free) 酶 | 2000U/支 |
Thermo | 12574026 | SuperScriptIII One-Step RT-PCR含Taq DNA 酶 | 100次/盒 |
QIAGEN | 57704 | QIAamp MinElute Virus Spin Kit(50)試劑盒 | 50次/盒 |
Promega | P2077 | T7 RNA Polymerase 聚合酶 | 5000U/支 |
Corning | 21-031-CVR | DPBS（1x）緩沖液(不含鈣鎂) | 500mL/瓶 |
NEB | M0363L | T5 核酸外切酶 | 5000U/組 |
歡迎您致電 華雅再生醫(yī)學旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司 ：1500 1904 520。紅榮微再-客服: 2395557778 經(jīng)銷商專員
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