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Monolith Ion-Exchange Columns for Superior High-Resolution Analytical and Preparative Protein Separations
The ProSwift SCX monolith column line has been expanded to include a new 1 x 50 mm column. As with all ProSwift monoliths, the fast mass transfer allows for fast protein separations. ProSwift monoliths offer excellent separations using fast gradients at low and high flow rates, which improve productivity.
ProSwift Monolith Ion Exchange columns are the column of choice for fast separations with high resolution for protein separation and purification, delivering higher resolution than porous bead-based columns while providing high capacity. ProSwift IEX monoliths provide the outstanding resolving power of nonporous analytical media combined with fast separation performance and exhibit reproducibility even after hundreds of runs.
Features of the ProSwift IEX monoliths:
High resolution
High loading capacity
Fast separations
Wide range of operational flow rates
Excellent stability over a wide pH range
Outstanding reproducibility and ruggedness
Optimal performance in a broad variety of applications
High throughput and improved productivity
ProSwift polymer monolith media are uniquely suited for separation of proteins. Each monolith is a single cylindrical, sponge-like polymer rod containing an uninterrupted, interconnected network of flow-through channels of a specific pore size. These large channels combined with the monolith’s nonporous surfaces result in fast mass-transfer, high-resolution, and fast protein separations. The unique globular morphology of the polymer medium provides its high capacity.
| ProSwift Monolith Ion Exchange 4.6 x 50 mm Specifications | ||||
|---|---|---|---|---|
| Specifications: | Spec Description: | |||
| Column Name: | Proswift WAX-1S | ProSwift SAX-1S | ProSwift WCX-1S | Proswift SCX-1S |
| Column Dimension (i.d. × length) | 4.6 × 50 mm | 4.6 × 50 mm | 4.6 × 50 mm | 4.6 × 50 mm |
| Base Matrix Material | Polymethacrylate | Polymethacrylate | Polymethacrylate | Polymethacrylate |
| Surface Chemistry | Tertiary amine | Quaternary amine | Caboxylic acid | Sulfonic acid |
| Protein Binding Capacity per mL of Polymer | 18 mg/mL BSA | 18 mg/mL BSA | 23 mg/mL BSA | 30 mg/mL BSA |
| Bed Height | 44 mm | 45 mm | 42 mm | 42 mm |
| Column Volume | 0.73 mL | 0.75 mL | 0.70 mL | 0.70 mL |
| pH Range | 2-12 | 2-12 | 2-12 | 2-12 |
| Recommended Flow Rate | 0.5 - 1.5 mL/min | 0.5 - 1.5 mL/min | 0.5 - 1.5 mL/min | 0.5 - 1.5 mL/min |
| Maximum Flow Rate | 2 mL/min | 2 mL/min | 2 mL/min | 2 mL/min |
| Maximum Pressure | 1000 psi 6.9 Mpa | 1200 psi 8.2 Mpa | 1000 psi 6.9 Mpa | 1000 psi 6.9 Mpa |
| Operating Pressure | <500 psi 3.4 Mpa | <500 psi 3.4 Mpa | <500 psi 3.4 Mpa | <500 psi 3.4 Mpa |
| Solvent Compatibility | Most common organic solvents | Most common organic solvents | Most common organic solvents | Most common organic solvents |
| Temperature | 60° C | 70° C | 60° C | 60° C |
| ProSwift Monolith Ion Exchange 1.0 x 50 mm Specifications | ||||
|---|---|---|---|---|
| Specifications: | Spec Description: | |||
| Column Name: | Proswift WAX-1S | ProSwift SAX-1S | ProSwift WCX-1S | ProSwift SCX-1S |
| Column Dimension (i.d. × length) | 1 × 50 mm | 1 × 50 mm | 1 × 50 mm | 1 × 50 mm |
| Base Matrix Material | Polymethacrylate | Polymethacrylate | Polymethacrylate | Polymethacrylate |
| Surface Chemistry | Tertiary amine | Quaternary amine | Caboxylic acid | Sulfonic acid |
| Protein Binding Capacity per mL of Polymer | 18 mg/mL BSA | 18 mg/mL BSA | 23 mg/mL Lysozyme | 13 mg/mL Lysozyme |
| Bed Height | 50 mm | 50 mm | 50 mm | 50 mm |
| Column Volume | 0.039 mL | 0.039 mL | 0.039 mL | 0.039 mL |
| pH Range | 2-12 | 2-12 | 2-12 | 2-12 |
| Recommended Flow Rate | 0.05 - 0.25 mL/min | 0.05 - 0.25 mL/min | 0.05 - 0.20 mL/min | 0.05 - 0.25 mL/min |
| Maximum Flow Rate | 0.3 mL/min | 0.3 mL/min | 0.25 mL/min | 0.3 mL/min |
| Maximum Pressure | 2000 psi 13.8 Mpa | 2000 psi 13.8 Mpa | 2000 psi 13.8 Mpa | 2000 psi 13.8 Mpa |
| Operating Pressure | <1500 psi 10.4 Mpa | <1500 psi 10.4 Mpa | <1000 psi 6.8 Mpa | <1500 psi 6.8 Mpa |
| Solvent Compatibility | Most common organic solvents | Most common organic solvents | Most common organic solvents | Most common organic solvents |
| Temperature | 60° C | 60° C | 60° C | 60° C |