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813次兔S100-A5蛋白(S100A5)ELISA kit
產(chǎn)品類型:
ELISA Kit
S100D, S100 calcium-binding protein A5
96T
Rabbit
S100 calcium binding protein A5
S100A5
15.6 pg/ml-1000 pg/ml
3.9 pg/ml
1-5h
50-100ul
450 nm
兔S100-A5蛋白(S100A5)ELISA kit This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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