Wnt糖蛋白是存在于多種生物體內的一種細胞外配體，Wnt作為形態(tài)發(fā)生素通過激發(fā)細胞內遠離信號發(fā)送區(qū)域的濃度依賴反應控制胚胎形態(tài)發(fā)育。Wnt通路調節(jié)動物發(fā)育過程中的多個重要環(huán)節(jié)，例如細胞增殖、細胞遷徙及細胞分化。Wnt蛋白通過自分泌或旁分泌作用與位于細胞膜上的受體相結合，激活細胞內信號通路調節(jié)靶基因的表達，對細胞的增殖、分化、遷移、極性化和凋亡起到重要作用。

StemRD W3A-M-100 WNT-3a,Mouse,100ug 產品簡介
wnt通路是細胞增殖分化的關鍵調控環(huán)節(jié)，在胚胎發(fā)育和腫瘤發(fā)生中起著重要作用。wnt途徑參與了基因表達調節(jié)、細胞遷移粘附、細胞極化等過程，同時還與其它信號通路存在交叉協(xié)同。Wnt/β-catenin通路在進化過程中高度保守，此通路的主要分子構成及相關調控機制已得到基本闡明。對神經系統(tǒng)而言已有足夠證據(jù)顯示此通路參與了對神經前體細胞增殖，分化以及決定細胞命運的調控。
Wnt-3a 干細胞生長分化因子是Wnt基因家族中重要成員，zui近的一些研究發(fā)現(xiàn)， Wnt-3a 干細胞生長分化因子信號蛋白對神經干細胞的定向分化的調控作用表現(xiàn)的十分明顯。小鼠WNT-3a 干細胞生長分化因子蛋白包含328個氨基酸。

StemRD W3A-M-100 小鼠WNT-3a 蛋白因子，100ug 產品特性
? StemRD W3A-M-100 小鼠WNT-3a 蛋白因子，100ug 干細胞生長分化因子活性經過NIH 3T3 細胞鑒定，純度高
? StemRD W3A-M-100 小鼠WNT-3a 蛋白因子，100ug干細胞生長分化因子生物活性在NIH 3T3 細胞經過TCF報告基因實驗鑒定The EC50為 50–150 ng/ml
? StemRD W3A-M-100 小鼠WNT-3a 蛋白因子，100ug 干細胞生長分化因子產品經過SDS-PAGE 和HPLC雙層鑒定純度大于90%

StemRD W3A-M-100 WNT-3a,Mouse,100ug 保存方法
The lyophilized protein is stable for at least 6 months if stored at -80 degreeC. Reconstituted protein is stable for at least two weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.

StemRD W3A-M-100 WNT-3a,Mouse,100ug 產品英文描述
WNT-3a is a member of the WNT family of signaling proteins that play a key role in embryonic development and the integrity of adult tissues. StemRD W3A-M-100 WNT-3a,Mouse,100ug is a prototypic canonical WNT that signals through the β-catenin pathway. The predicted size of mouse StemRD W3A-M-100 WNT-3a,Mouse,100ug is a monomeric protein containing 333 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of 40 kDa by SDS-PAGE analysis under nonreducing conditions. StemRD’s product W3A-M-100 WNT-3a,Mouse,100ug is expressed from a mouse cell line overexpressing mouse WNT-3a. Purification is performed with a proprietary process that is distinct from the published method.
? StemRD W3A-M-100 WNT-3a,Mouse,100ug Purity: Greater than 90% as determined by SDS-PAGE analysis
? StemRD W3A-M-100 WNT-3a,Mouse,100ug Biological Activity: The activity was determined by using a TCF reporter gene assay in NIH3T3 cells. The EC50 ranges from 50 - 150 ng/ml. Country of Origin USA