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NUCLEOSIL® PPN

Silica-based HPLC phases for RP chromatography of biological macromolecules

Features

Silica-based reversed phase materials with polymerically bonded alkyl chains,
exclusively hydrophobic interactions

NUCLEOSIL® 100-5 C18 PPN: octadecyl phase, particle size 5 µm, pore size 100 Å ? USP L1
Dynamic protein binding capacity per g packing: 8 mg BSA, 64 mg cytochrome C
Suited for the separation of peptides and proteins up to about 40 kD, also suited for basic peptides

NUCLEOSIL® 500-5 C18 PPN: octadecyl phase, particle size 5 µm, pore size 500 Å ? USP L1
Dynamic protein binding capacity per g packing: 22 mg BSA, 40 mg cytochrome C
Especially suited for large peptides and medium-size hydrophilic proteins

For more applications click here.

pH working range 1–9, max. working pressure 250 bar
Eluent in column methanol

Ordering information

Length ->

125 mm

250 mm

CC guard columns*

EC analytical columns   

NUCLEOSIL® 100-5 C18 PPN

4 mm ID

720251.40

720252.40

721594.40

NUCLEOSIL® 500-5 C18 PPN

4 mm ID

720257.40

720258.40

721687.40

* CC 8/4 guard column cartridges are used for EC columns of 4 mm ID with guard column adapter EC (REF 721359).
Guard columns in packs of 2, EC columns in packs of 1