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NUCLEOSIL® NH2 / NH2 RP 液相色譜柱
Nucleosil 高性能硅膠，特殊技術(shù)鍵合，使NH2鍵合相在水相介質(zhì)中穩(wěn)定，低流失，保證柱子長壽命※ 鍵合相采用非端基封尾，具有更廣泛的選擇性※ 有100, 120 and 300 Å，三種孔徑；有3um, 5um, 7um, 10um 四種粒度
產(chǎn)品簡介
詳細(xì)介紹
Nucleosil NH2 氨基柱
※ Nucleosil 高性能硅膠，特殊技術(shù)鍵合，使NH2鍵合相在水相介質(zhì)中穩(wěn)定，低流失，保證柱子長壽命※ 鍵合相采用非端基封尾，具有更廣泛的選擇性※ 有100, 120 and 300 Å，三種孔徑；有3um, 5um, 7um, 10um 四種粒度
※ pH stability at 20 °C: 2–8, carbon content 3.5 %※ 可以使用多種流動(dòng)相體系，分析不同性質(zhì)樣品：
* 正相流動(dòng)相（正乙烷，二氯乙烯，異丙醇），分析苯胺取代物，酯類和有機(jī)氯農(nóng)藥
* 水和有機(jī)物作為正相流動(dòng)相，可以分析極性樣品，如：糖類、植物甙類
* 反相中添加緩沖鹽，作為弱離子交換體系可以分析有機(jī)酸類，陰離子化合物
NUCLEOSIL® NH2 / NH2 RP |
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Amino modified silica phases for HPLC · USP L8 |
Features | |
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| For applications click here. |
產(chǎn)品應(yīng)用:
雙糖檢測 |
色譜柱：Nucleosil 100-5 NH2, 250×4 mm 流動(dòng)相：乙腈：水=75:25 流 速：1毫升/分鐘
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樣 品：1. 甲基葡萄糖苷 2. 果糖 3. 葡萄糖 4. 蔗糖 5. 乳糖 6. 麥芽糖 |
HPLC檢測 Curcumin （姜黃素）及相關(guān)組份 |
色譜柱：Nucleosil 100-5 NH2, 250×4.6 mm ID 流動(dòng)相：96% 乙醇 流 速：1.2毫升/分鐘 檢測器：fluorescence, excitation 420nm, emission 470nm. The curcumino ids can also be detected by UV-VIS spectroscopy at 254 or 420nm.
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樣 品：1. Bisde methoxycurcumin 2. Demethoxycurcumin 3.Curcumin |
發(fā)酵液中多糖的檢測 |
色譜柱：Nucleosil 100-5 NH2, 250×4 mm 流動(dòng)相：乙腈：水=65:35 流 速：2毫升/分鐘 溫 度：80℃ |
訂貨信息:
EC standard columns for analytical HPLC |
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Ordering information | |
Eluent in column (except for NUCLEOSIL® 100-5 NH2 RP) is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is necessary to rinse the column with THF first. |
ID | Particle size | Pore size |
| Length 250 mm |
| EC guard | CC guard |
NUCLEOSIL® 100-3 NH2 (eluent in column n-heptane) |
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4.6 mm | 3 µm | 100 Å |
| 720275.46 |
| 721933.30 | 721122.40 |
NUCLEOSIL® 100-5 NH2 RP (eluent in column acetonitrile – water) |
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4.6 mm | 5 µm | 100 Å |
| 720095.46RP |
| 721155.30 | 721605.40RP |
NUCLEOSIL® 100-5 NH2 (eluent in column n-heptane) |
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4.6 mm | 5 µm | 100 Å |
| 720095.46 |
| 721020.30 | 721605.40 |
NUCLEOSIL® 100-10 NH2 (eluent in column n-heptane) |
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4.6 mm | 10 µm | 100 Å |
| 720025.46 |
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Columns/pack -> |
| 1 |
| 3 | 3 | ||
* EC 4/3 guard column cartridges are used for EC columns of 4.6 mm ID with the Column Protection System guard column holder (REF 718966). | |||||||
Custom-packed EC columns with other dimensions are available on request. | |||||||
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Phase |
| Carbon content | Pore size | Particle size | Pack of 10 g | Pack of 100 g | |
NUCLEOSIL 100-5 NH2 |
| 3.5 % | 100 Å | 5 µm | 712200.10 | 712200.100 | |
NUCLEOSIL 100-10 NH2 |
| 3.5 % | 100 Å | 10 µm | 712210.10 | 712210.100 | |
NUCLEOSIL 120-7 NH2 |
| ~ 2 % | 120 Å | 7 µm | 712610.10 | 712610.100 | |
NUCLEOSIL 300-7 NH2 |
| ~ 2 % | 300 Å | 7 µm | 712919.10 | 712919.100 | |
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