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便攜式調(diào)制葉綠素?zé)晒鈨xPAM-2100
  • 便攜式調(diào)制葉綠素?zé)晒鈨xPAM-2100

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更新時(shí)間:2024-10-12 21:00:07

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( 聯(lián)系我們,請說明是在 化工儀器網(wǎng) 上看到的信息,謝謝!)

*Z的調(diào)制熒光儀PAM-2000的升級版,是目前Z高檔的光纖型熒光儀!具備強(qiáng)大的單機(jī)操作功能,是野外光合作用研究的*儀器!

pam-2100——野外光合作用研究的*儀器
schreiber
教授因發(fā)明pam系列調(diào)制葉綠素?zé)晒鈨x而獲得首屆光合作用協(xié)會(ispr)創(chuàng)新獎(jiǎng)


1983年,walz公司*科學(xué)家、德國烏茲堡大學(xué)的ulrich schreiber教授設(shè)計(jì)制造了**臺調(diào)制熒光儀——pam-101/102/103,使在自然光下測量葉綠素?zé)晒獬蔀楝F(xiàn)實(shí),解決了科學(xué)界近50年的技術(shù)瓶頸。pam-101/102/103迅速在植物生理、生態(tài)、農(nóng)學(xué)、林學(xué)、水生生物學(xué)等領(lǐng)域得到廣泛應(yīng)用,出版了大量高水平研究文獻(xiàn)。但該儀器比較笨重,不易帶到野外。

1992年,walz公司*科學(xué)家、調(diào)制熒光儀發(fā)明人、德國烏茲堡大學(xué)的ulrich schreiber教授設(shè)計(jì)制造了**臺便攜式調(diào)制熒光儀——pam-2000,并且在植物生理生態(tài)學(xué)等科研領(lǐng)域得到廣泛應(yīng)用,此后十幾年中成為zui的調(diào)制熒光儀。

2003年,walz公司在保留pam-2000所有功能和優(yōu)點(diǎn)的基礎(chǔ)上,結(jié)合技術(shù),將pam-2000升級到了pam-2100。


系統(tǒng)描述
pam-2100采用了*的調(diào)制技術(shù)飽和脈沖技術(shù),從而可以通過選擇性的原位測量葉綠素?zé)晒鈦頇z測植物光合作用的變化。pam-2100的調(diào)制測量光足夠低,可以只激發(fā)色素的本底熒光而不引起任何的光合作用,從而可以真實(shí)的記錄基礎(chǔ)熒光fo。pam-2100具有很強(qiáng)的靈敏度和選擇性,使其即使在很強(qiáng)的、未經(jīng)濾光片處理的環(huán)境下(如全日照甚至是10000 μmol m-2 s-1的飽和光強(qiáng)下)也可測定熒光產(chǎn)量而不受到干擾。因此,pam-2100不但適合在實(shí)驗(yàn)室人工控制的環(huán)境下測量,還可以在自然環(huán)境中甚至是強(qiáng)烈的全光照條件下開展野外科學(xué)研究。

pam-2100是非常便攜、強(qiáng)大的測量系統(tǒng),它將各種光學(xué)和電子元件組裝在一個(gè)24 cm×10.5 cm×11 cm的外殼中。測量光由655 nm的發(fā)光二極管(led)發(fā)出,可在低頻(600 hz)和高頻(20 khz)間自動(dòng)切換。光化光(光合生物實(shí)際可吸收利用進(jìn)行光合作用的可見光)由鹵素?zé)簦ò坠猓┗蚣t光led(655 nm)提供。遠(yuǎn)紅光(735 nm,促進(jìn)光系統(tǒng)i迅速消耗掉在pq處累積的電子)由led發(fā)出。

pam-2100的按鍵操作非常簡單。基礎(chǔ)測量只需單健操作。數(shù)據(jù)在內(nèi)置電腦中自動(dòng)分析、存儲并且在顯示屏上顯示。除了“參數(shù)窗"外,在“動(dòng)力學(xué)窗"還可顯示曲線的實(shí)時(shí)變化。

pam-2100利用光纖進(jìn)行信號傳輸。光適應(yīng)葉夾2030-b(產(chǎn)品)上配備微型光量子/溫度傳感器,可在記錄熒光信號的同時(shí),同步記錄光合有效輻射(par)和溫度變化。

pam-2100內(nèi)設(shè)10個(gè)標(biāo)準(zhǔn)run(預(yù)先編好的間隔一定時(shí)間并按一定順序執(zhí)行特定命令的程序),用戶只需一次按鍵就可進(jìn)行復(fù)雜的實(shí)驗(yàn)。用戶還可對這些標(biāo)準(zhǔn)run進(jìn)行編輯得到自己的user-run(數(shù)量不限),來滿足特殊的實(shí)驗(yàn)需要。
pam-2100主機(jī)可以直接連接電腦(圓口)鍵盤,在野外現(xiàn)場,可以根據(jù)實(shí)驗(yàn)需要,不需電腦就可以進(jìn)行特殊程序的編輯。

pam-2100還可以設(shè)定單機(jī)操作軟件da-2100自動(dòng)間隔一定時(shí)間執(zhí)行某個(gè)run或user-run,而run是可以無限擴(kuò)展的,因此,可以說pam-2100的功能幾乎可以無限擴(kuò)展。只要將主機(jī)和葉夾(均可固定在三角架上)固定好,按一次按鍵,(人不在現(xiàn)場看守)儀器可以自動(dòng)進(jìn)行非常復(fù)雜的測量過程。

此外,pam-2100主機(jī)還可以連接電腦顯示器或投影儀放大顯示,非常適合進(jìn)行教學(xué)使用。


特點(diǎn)
1) 聲譽(yù)*的pam-2000的升級版
2) 精巧、準(zhǔn)確、迅速、操作簡便的高級光合作用檢測設(shè)備
3) 可單機(jī)操作(采用內(nèi)置電腦,da-2100軟件記錄),可連接外置電腦操作(windows操作軟件pamwin)
4) 便攜式設(shè)計(jì),帶大屏幕液晶顯示屏(可顯示曲線變化)和20個(gè)按鍵
5) 強(qiáng)大的數(shù)據(jù)收集、分析和存貯功能
6) 可以預(yù)先編寫和設(shè)定程序,進(jìn)行特殊研究目的測量
7) 內(nèi)置鋰電池可滿足長時(shí)間野外工作需要,并可連接外置12 v電池
8) 多種葉夾可供選擇,設(shè)計(jì)的光適應(yīng)葉夾2030-b可同時(shí)記錄par和溫度變化
9) 光源選擇:自然光,內(nèi)置光源(提供測量光、光化光、飽和脈沖和遠(yuǎn)紅光),可選外置鹵素?zé)艄庠矗ㄌ貏e適合野外研究)


功能
1) 可測熒光誘導(dǎo)曲線的快速上升動(dòng)力學(xué)o-i-d-p相和o-j-i-p相
2) 可測熒光誘導(dǎo)曲線的慢速下降動(dòng)力學(xué)并進(jìn)行淬滅分析(fo, fm, fv/fm, f, fm, fo’, df/fm’, qp, qn, npq, retr等)
3) 可測光響應(yīng)曲線和快速光曲線(rlc)
4) 儀器內(nèi)置一系列標(biāo)準(zhǔn)實(shí)驗(yàn)(run1~run10),用戶可對其進(jìn)行編輯建立自己的user-run
5) 可在線檢測植物、微藻、地衣、苔蘚等的光合作用變化
6) 單機(jī)操作功能強(qiáng)大,特別適合野外操作,實(shí)驗(yàn)室內(nèi)單機(jī)操作時(shí)可連接電腦顯示器或投影儀放大顯示


應(yīng)用領(lǐng)域
儀器設(shè)計(jì)特別適合野外使用,可用于研究光合作用機(jī)理、各種環(huán)境因子(光、溫、營養(yǎng)等)對植物生理狀態(tài)的影響、植物抗逆性(干旱、冷、熱、澇、uv、病毒、污染、重金屬等)、植物的長期生態(tài)學(xué)變化等。在植物生理學(xué)、植物生態(tài)學(xué)、植物病理學(xué)、農(nóng)學(xué)、林學(xué)、園藝學(xué)、水生生物學(xué)、環(huán)境科學(xué)、毒理學(xué)、微藻生物技術(shù)、極地植物光合作用研究等領(lǐng)域有著廣泛應(yīng)用。


10個(gè)標(biāo)準(zhǔn)run
run 1:測量實(shí)際量子產(chǎn)量yield(δf/fm’)
run 2:測量zui大量子產(chǎn)量fv/fm
run 3:記錄誘導(dǎo)曲線并進(jìn)行淬滅分析(采點(diǎn)率10 ms/點(diǎn))
run 4:記錄誘導(dǎo)曲線并進(jìn)行淬滅分析(采點(diǎn)率30 ms/點(diǎn))
run 5:qn 的馳豫動(dòng)力學(xué)
run 6:快速誘導(dǎo)動(dòng)力學(xué)o-i-d-p相(線性時(shí)間)
run 7:快速誘導(dǎo)動(dòng)力學(xué)o-j-i-p相(對數(shù)時(shí)間)
run 8:光響應(yīng)曲線(需76 min)(稍加編輯即可測量快速光曲線)
run 9:光響應(yīng)曲線(需33 min)(稍加編輯即可測量快速光曲線)
run 10:儀器自檢


用戶可根據(jù)實(shí)驗(yàn)需要,自行修改或編制程序;如需幫助,請!

無限擴(kuò)展的編程功能,單機(jī)操作功能更加強(qiáng)大?。?!


單機(jī)操作時(shí)記錄的熒光誘導(dǎo)曲線加淬滅分析、以及相關(guān)熒光參數(shù)的變化


單機(jī)操作時(shí)記錄的快速熒光誘導(dǎo)動(dòng)力學(xué)曲線



技術(shù)參數(shù)
測量光
:紅色發(fā)光二極管(led),650 nm,標(biāo)準(zhǔn)強(qiáng)度0.1 μmol m-2 s-1 par;調(diào)制頻率0.6或20 khz,自動(dòng)轉(zhuǎn)換。
光化光
? 紅色led,665 nm,zui大連續(xù)光強(qiáng)600 μmol m-2 s-1 par
? 鹵素?zé)簦?v/20w,zui大連續(xù)光強(qiáng)8500 μmol m-2 s-1 par
飽和脈沖:鹵素?zé)簦?v/20w,zui大飽和閃光強(qiáng)度μmol m-2 s-1 par。
遠(yuǎn)紅光:led,730 nm,zui大強(qiáng)度15 w m-2。
信號檢測:pin-光電二極管,帶短波截止濾光片(λ>710 nm);選擇性鎖相放大器(設(shè)計(jì))。
數(shù)據(jù)存儲:128 mb
測量參數(shù):fo, fm, fm’, f, fo’, fv/fm(max. yield), δf/fm’(yield), qp, qn, npq, etr, par和葉溫等。

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