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Invitrogen 18418-012 Oligo（dT）12-18 Primer 簡(jiǎn)介：
產(chǎn)品名稱：Oligo(dT)12-18 Primer
品 牌：Invitrogen
國(guó)產(chǎn)/進(jìn)口：進(jìn) 口
銷售商：上海慧穎生物科技有限公司
貨 號(hào)：18418-012
規(guī) 格：25ug
儲(chǔ)存溫度：-20°C
特惠價(jià)格：致 電
庫(kù)存狀態(tài)：現(xiàn) 貨
上?；鄯f生物科技有限公司專業(yè)供應(yīng)Invitrogen 18418-012 Oligo（dT）12-18 Primer ，常備現(xiàn)貨，*。我公司主營(yíng)Life Invitrogen產(chǎn)品，囊括TRIZOL LS Reagent 、B-27添加劑、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、小牛血清、豬血清、馬血清等，如有需要，詳詢，：/。
Description:
Oligo(dT)12-18 Primer hybridizes to the poly(A) tail of mRNA and is suitable
for use as a primer for first stand cDNA synthesis with reverse transcriptase.
The primer is supplied in DEPC-treated water at a concentration of 0.5 μg/μl.
The primer is phosphorylated at the 5' end.
Component: Oligo(dT)12-18 Primer
Recommended Amount: We recommend using 0.5 μg of oligo(dT)12-18 primer per μg of poly(A)+
RNA. For total RNA, we recommend 0.5 μg of primer for < 5 μg of RNA.
Functional Assay Conditions :
1 μl oligo(dT)12-18 primer (0.5 μg/μl)
1.0 μg poly(A)+ RNA
Sterile distilled water
Heat mixture to 70°C for 10 min, and quick chill on ice.
4 μl 5X first strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mMMgCl2]
2 μl 0.1 M DTT
1 μl dNTPs (10 mM each dNTP)
1 μl [α-32P]dCTP (1 μCi/μl)
200 units SuperScript? RT (Cat. No. 18053-017) or M-MLV RT (Cat. No.28025-013/28025-021)
Total volume: 20 μl
Incubate at 37°C for 1 h.
Quality Control :This product is qualified in a first strand cDNA reaction.
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