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詳細(xì)介紹
CELLBLOKS細(xì)胞間相互作用培養(yǎng)板是一種多器官/細(xì)胞類型的模塊化“即插即用”共培養(yǎng)技術(shù)，可在標(biāo)準(zhǔn)的體外環(huán)境中模擬器官的微環(huán)境。該技術(shù)為研究人員提供了一種新穎的方法，可以更好地預(yù)測人類對新藥，化妝品，食品和病毒的反應(yīng) 。
該平臺(tái)以標(biāo)準(zhǔn)孔板格式設(shè)計(jì)，使其與標(biāo)準(zhǔn)讀數(shù)設(shè)備兼容。
CELLBLOKS®是一種多器官/細(xì)胞類型的模塊化“即插即用”共培養(yǎng)技術(shù)。 它用于在標(biāo)準(zhǔn)體外環(huán)境中模擬器官微環(huán)境的目的。 通過使用標(biāo)準(zhǔn)灌注搖桿，它可以研究在2D，3D細(xì)胞生長條件以及靜態(tài)或流動(dòng)系統(tǒng)中進(jìn)行測定的可行性，使用CELLBLOKS®，可以在Barrier Pod™或Circulatory Blocks™上生長不同的細(xì)胞類型，它們模仿屏障器官或系統(tǒng)循環(huán)的器官。 然后可以通過多種方式將包含器官特定細(xì)胞的模塊彼此連接起來，以模擬復(fù)雜的
器官-器官相互作用。 例如，腸細(xì)胞在屏障塊上生長，而肝和肺細(xì)胞在循環(huán)塊上 。
輕松進(jìn)行細(xì)胞間通訊研究CELLBLOKS®平臺(tái)具有SBS標(biāo)準(zhǔn)組織培養(yǎng)孔板的尺寸。 它具有四個(gè)單獨(dú)的細(xì)長通道，并且可以放置三個(gè)單獨(dú)的模塊。 每個(gè)通道都充滿了介質(zhì)（3-5毫升），以允許各個(gè)模塊之間的細(xì)胞通信。
多細(xì)胞和器官型共培養(yǎng)
配置組合矩陣進(jìn)行中的研究的多個(gè)細(xì)胞類型的相互作用
CELLBLOKS®系統(tǒng)允許您設(shè)置三向組合的可能性，這意味著系統(tǒng)中的多個(gè)細(xì)胞類型都可以在一個(gè)板中以兩個(gè)或三個(gè)訓(xùn)練分別進(jìn)行測試。可以，CELLBLOKS®是使研究 CELLBLOKS®矩陣使您能夠識(shí)別和選擇正確的細(xì)胞類型組合，從而在早期進(jìn)行進(jìn)一步研究，從而幫助您節(jié)省時(shí)間和資金。
快速簡單的分析
實(shí)時(shí)可視化和監(jiān)控
開放式訪問允許您實(shí)時(shí)監(jiān)控實(shí)驗(yàn)。可以通過標(biāo)準(zhǔn)共聚焦顯微鏡對細(xì)胞進(jìn)行實(shí)時(shí)成像而不會(huì)被破壞。另外，當(dāng)細(xì)胞相互作用時(shí)，可以收集培養(yǎng)基用于進(jìn)一步分析，從而可以研究長期作用。
節(jié)省時(shí)間
易于設(shè)置的“即插即用”模塊設(shè)計(jì)也便于實(shí)時(shí)修改實(shí)驗(yàn)。在不干擾培養(yǎng)中其他細(xì)胞的情況下，可隨時(shí)從基室中取出并替換單個(gè)細(xì)胞生長阻滯物。這樣一來，您就可以有更多時(shí)間來生成所需的數(shù)據(jù)，而從一開始就可以花費(fèi)更少的時(shí)間來重置實(shí)驗(yàn)。
更具生產(chǎn)力
在一個(gè)平臺(tái)上同時(shí)執(zhí)行多個(gè)實(shí)驗(yàn)
每個(gè)平臺(tái)都有四個(gè)單獨(dú)的通道，可以在一臺(tái)設(shè)備中同時(shí)運(yùn)行單獨(dú)的單元間交互實(shí)驗(yàn)，從而在更短的時(shí)間內(nèi)實(shí)現(xiàn)更高的生產(chǎn)率。
再現(xiàn)性更高
通過在整個(gè)實(shí)驗(yàn)中維持相似的環(huán)境來產(chǎn)生可靠的數(shù)據(jù)
CELLBLOKS®以標(biāo)準(zhǔn)SBS平板格式設(shè)計(jì)，可與常規(guī)的CO2恒溫箱和讀數(shù)設(shè)備配合使用。這使您能夠醉大程度地減少標(biāo)準(zhǔn)培養(yǎng)皿和讀出設(shè)備之間的環(huán)境差異，從而為您提供更可靠和可重復(fù)的數(shù)據(jù)。
氣密性減少污染，減少介質(zhì)蒸發(fā)并避免pH漂移的更好方法CELLBLOKS®腔室具有新設(shè)計(jì)的“夾氣式”“氣密”功能，帶有兩個(gè)側(cè)夾，可輕松在腔室周圍形成100％的氣密密封 。
蓋子上裝有PTFE空氣過濾器和O形圈密封件，以過濾到達(dá)細(xì)胞培養(yǎng)物中的空氣。 養(yǎng)殖中的氣體只能通過選擇性滲透的空氣過濾器（0.2毫米）進(jìn)行交換
這樣可以減少細(xì)胞潛在的污染，培養(yǎng)基的蒸發(fā)以及實(shí)驗(yàn)過程中pH的波動(dòng)。 與其他細(xì)胞培養(yǎng)平臺(tái)相比，Air-Lid技術(shù)是手創(chuàng)的
-實(shí)現(xiàn)更可重復(fù)的數(shù)據(jù)采集和處理優(yōu)勢
How does it work?
CELLBLOKS®, is a multi-organ/cell type modular “plug and play” co-culture technology. It serves the purpose of emulating the organ microenvironment in a standard in-vitrosetting. It provides the feasibility of studying the assays in 2-D, 3-D cell growth condition, and in static or flow system, by using a standard perfusion rocker
Using CELLBLOKS® different cell types can be grown on Barrier Blocks™ or Circulatory Blocks™ that emulate barrier organs or those in systematic circulation. Blocks containing organ specific cells can then be connected to each other in variety of ways to simulate complex organ-organ interactions. For instance, Intestinal cell are grown on Barrier Blocks, whereas Liver and lung cells in Circulatory Blocks.
In this way you can quickly and easy set up an experiment in the lab to simulate complex body conditions, for example, to test how organs communicate to each other when they are exposed to a test drug
THE PLATFORM
Cell-cell communication studies made easy
CELLBLOKS® platform has dimensions of a SBS standard Tissue Culture well plate. It has four sperate elongated channels with location for three separate Blocks. Each channel is filled with media (3-5 ml) to allow the cell-cell communication between the Blocks
THE CELL GROWTH BLOCKS
Achieve better representation of different Organtype communications
1. Circulatory Blocks™ provide a flat plastic surface for cells to grow with side circulatory windows on the walls allowing selective media diffusion (both inlet and outlet, simulating organs in systematic circulation e.g. liver, brain, heart, lung).
2. Barrier Blocks™ contain a selective permeable membrane on the bottom of the Block, allowing cell to proliferative on basolateral membrane (simulating epithelial cells and tissues).
3. Blank Blocks™ have the same flat surface as the Circulatory Blocks for cell growth but no inlet or outlet for media diffusion. Blank Blocks are used to isolate cell cultures from other compartments and are often used as controls.
In addition, the modular nature of the device allows the removal of one or more cell growth Blocks from the chamber to perform separate analysis without disturbing others cell compartments in culture.
Furthermore, as it provides separate compartments for each cells of a co-culture that cross-communicate among each other through media without leaving their compartment (unlike the conventional co-culture models), this permits user to study individual biology of each cell (e.g. protein, imaging, DNA).
FILTERED AIR FLOW
Better ways to reduce contamination, media evaporation and avoid pH drifts
The CELLBLOKS® chamber comes with new design Clip-Closure "Air-Lid" feature with two side clips that make it easy to create 100% hermetic seal around the chamber.
PTFE air filters and an o-ring seal have been incorporated in the lid in order to filter the air reaching cell cultures. Gas is in cultures is only exchanged through selectively permeable air filters (0.2 micorn) which reduce potential contamination of cells, evaporation of media, and fluctuations of pH during experimentation. In contrast to other cell culure platforms the Air-Lid technology is first-of-its-kind - enabling more reproducible data acqusitions and handling advantages.
How do the molecules diffuse between the Blocks
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