細(xì)胞細(xì)菌實(shí)時(shí)表型反應(yīng)測(cè)量系統(tǒng)

細(xì)胞代謝和監(jiān)測(cè)變得容易，我們的無(wú)標(biāo)記多通道測(cè)定法以非凡的度實(shí)時(shí)測(cè)量細(xì)胞和病原體的特定代謝表型。

使用微量量熱法實(shí)時(shí)監(jiān)測(cè)生物膜代謝。

快速抗菌藥敏測(cè)試

對(duì)抗生素功效的一次或多次測(cè)試的細(xì)菌反應(yīng)的高靈敏度測(cè)量–監(jiān)測(cè)生死的準(zhǔn)確性和速度達(dá)到新水平。

• 實(shí)時(shí)直接代謝讀數(shù)，無(wú)需標(biāo)記或添加
• 表型反應(yīng)可提供易感和耐藥菌株的確切行為
• 快速檢測(cè)時(shí)間可快速診斷
• 連續(xù)和定量可視化的殺菌和抑菌效果
• AST的多功能抗生素組合測(cè)試

Thermogram

calScreener™ raw data

Heat over time curve

Integrated data, equivalent to bacterial growth curve

Escherichia coli planktonic growth in Mueller Hinton media. Normalized data vs control.

calScreener measures the power produced from the sample in Joules per second or µW and gives a specific curve dependent on the specimen and the culture media. When calScreener data is integrated over time the heat over time curve matches the conventional density growth curves from which the growth parameters can be calculated. Parameters such as maximum growth rate (the slope of the log phase in h-1) and the total biomass of the culture (Peak power in µW).

我們地測(cè)量生與死
直接代謝讀數(shù)–我們通過監(jiān)測(cè)暴露于抗生素的細(xì)菌的代謝變化來衡量生命的特征，calScreener™是wei一可以做到這一點(diǎn)并提供生長(zhǎng)動(dòng)力學(xué)和長(zhǎng)期細(xì)胞生存力讀數(shù)的解決方案

定義有效的組合治療
現(xiàn)有數(shù)量有限的抗菌劑，并且尚無(wú)用于組合療法測(cè)試的商業(yè)解決方案。 我們將為組合測(cè)試設(shè)定新的金標(biāo)準(zhǔn)

高靈敏度-提供準(zhǔn)確的數(shù)據(jù)
calScreener具有很高的靈敏度，并且可以連續(xù)監(jiān)測(cè)暴露于抗生素的細(xì)菌的代謝反應(yīng)

快速實(shí)時(shí)的響應(yīng)
不到8小時(shí)的抗生素敏感性以及正確測(cè)量細(xì)胞對(duì)細(xì)菌的殺傷和抑制作用意味著沒有因細(xì)胞碎片或裂解細(xì)胞引起的錯(cuò)誤反應(yīng)

P. aeruginosa

A. baumannii

P. aeruginosa and A. baumannii planktonic growth in Mueller Hinton media, same experimental conditions as for BMD.

The calScreener™ gives completely flexibility regarding the single or combo treatment, making AST most flexible and relevant. 

In these graphs, it can be observed the lack of effect in the metabolism of the culture of the single antibiotic used. However, there is a clear synergistic effect when using the combination of antibiotics in the bacterial metabolism. Prolonged observation of the activity makes it possible to study re-growth, biofilm formation and late-stage survival of population subsets.

 

 

 

The bacterial species and strain give rise to unique metabolic thermograms

 

P. aeruginosa

A. baumannii

K. pneumoniae

E. coli

Bacterial planktonic growth in Mueller Hinton media, same experimental conditions as for BMD. 

The unique heat flow footprint obtained in calScreener is dependent on the metabolism of the specimen, and this depends on the strain studied, as well as on the culture media and the availability of nutrients within it. calScreener can be employed for both gram-negative and gram-positive bacteria with equal sensitivity.

 

P. aeruginosa

A. baumannii

P. aeruginosa and A. baumannii planktonic growth in Mueller Hinton media, same experimental conditions as for BMD.

The calScreener™ gives completely flexibility regarding the single or combo treatment, making AST most flexible and relevant. 

In these graphs, it can be observed the lack of effect in the metabolism of the culture of the single antibiotic used. However, there is a clear synergistic effect when using the combination of antibiotics in the bacterial metabolism. Prolonged observation of the activity makes it possible to study re-growth, biofilm formation and late-stage survival of population subsets.

 

 

 

The bacterial species and strain give rise to unique metabolic thermograms

 

P. aeruginosa

A. baumannii

K. pneumoniae

E. coli

Bacterial planktonic growth in Mueller Hinton media, same experimental conditions as for BMD. 

The unique heat flow footprint obtained in calScreener is dependent on the metabolism of the specimen, and this depends on the strain studied, as well as on the culture media and the availability of nutrients within it. calScreener can be employed for both gram-negative and gram-positive bacteria with equal sensitivity.