Gastight box 氣密箱 CR1601

Gastight box 氣密箱 CR1601

氣密箱CR1601（內(nèi)夾）氣密箱內(nèi)夾（CR1601）使需要特定氣體環(huán)境（如較高的二氧化碳濃度或無(wú)活力培養(yǎng)）的微量平板發(fā)酵成為可能。內(nèi)部夾持系統(tǒng)與lowwell MTPs的夾持系統(tǒng)相同（見(jiàn)lowwell板蓋和夾持器），但該系統(tǒng)也可容納帶夾層蓋的深井板。氣密箱可安裝在Kuhner的振動(dòng)臺(tái)上，或在振動(dòng)臺(tái)上增加一些額外的孔后，安裝在所有其他類(lèi)型的振動(dòng)臺(tái)上，其結(jié)構(gòu)能夠承受高達(dá)300轉(zhuǎn)/分的持續(xù)振動(dòng)，振幅為25毫米，225轉(zhuǎn)/分的持續(xù)振動(dòng)，振幅為50毫米。由于軌道振動(dòng)條件的這些限制，我們建議使用24口井MTP和井蓋，或者使用方形半深井MTP和96口井MTP（CR 1596b）的通用井蓋。氣密箱有一個(gè)進(jìn)氣口和一個(gè)排氣口（均通過(guò)球閥），與標(biāo)準(zhǔn)的6毫米推入式連接器兼容。盒子氣密（在有機(jī)玻璃盒子和碳接地板之間使用三元乙丙橡膠密封），但不耐超壓或超壓超過(guò)0.1巴。因此，不能用真空循環(huán)使箱體厭氧，必須進(jìn)行沖洗。尺寸（接地板220x194 mm，總高度包括球閥220mm）允許進(jìn)出大多數(shù)通風(fēng)室（通過(guò)氣閘室：只要閥門(mén)打開(kāi)，就可以使用真空循環(huán)，或?qū)⒂袡C(jī)玻璃箱和鋁基板分別移入?yún)捬跏?，用聚四氟乙烯管（外?mm）在搖動(dòng)過(guò)程中進(jìn)行連續(xù)沖洗，總重量（包括微量滴定板和蓋）約為2.5kg，這意味著，也由于相對(duì)較高的重量點(diǎn)，多可將4-6個(gè)箱子安裝在一個(gè)振動(dòng)臺(tái)上（取決于振動(dòng)臺(tái)型號(hào)）。氣密箱可容納兩個(gè)帶夾層蓋的深井MTP，或六個(gè)帶夾層蓋的低井MTP。

 Gastight box CR1601 (with clamp inside) The gastight box with a clamp inside (CR1601) enables microtiter plate fermentations requiring a specific gas-atmosphere (e.g. a higher CO2 concentration, or anearobic c*tions). The clamp system inside is identical to the clamp system for lowwell MTPs (see Lowwell plate covers and clamps), but this system can also harbour deepwell plates with sandwich covers.The gastight box can be mounted onto shaking platforms from Kuhner, or - after adding some extra holes in the shaker platform - on all other shaker types, and has been constructed to withstand  continuous shaking up to 300 rpm at an amplitude of 25 mm, and 225 rpm at an amplitude of 50 mm. Because of these limitations in orbital shaking conditions, we advise to work either with 24 well MTPs and covers or with square half deepwell MTPs in combination with our universal covers for 96-well MTPs (CR 1596b)The gastight box has a gas inlet and a gas outlet (both via a ball-valve), that is compatible with standard 6 mm push-in connectors.The box is gastight (using an EPDM rubber sealing between the perspex box and the carbon groundplate), but is not resistant to overpressure or underpressure more than 0.1 bar. Therefore, the box can not be made anaerobic using vacuum cycles, but has to be flushed instead. The dimensions (groundplate 220x194 mm, total height including ball valves 220mm) allow the transfer in and out most anearobic chambers (via an airlock: vacuum cycles can be used provided the valves of the box are open, or the perspex box and the alu groundplate are transferred into the anaerobic chamber separately).Continuous flushing during shaking is feasible using PTFE tubing (outside diameter 6 mm).The total weight (including microtiter plates and covers) is approximately 2.5 kg, which implies that - also due to the relatively high heavy point - maximally 4-6 boxes can be fitted onto one shaking platform (depending on the shaker model).The gastight box can harbour either two deepwell MTPs with sandwich covers, or six lowwell MTPs with sandwich covers.

 注：楊清辰發(fā)布