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Hydrodynamics inside wells 225轉(zhuǎn)/分
Hydrodynamics inside wells 225轉(zhuǎn)/分
六方深井微板孔內(nèi)流體力學(xué)
對于6平方深孔微量滴定板,通常在25毫升和35毫升之間的培養(yǎng)體積,在搖床上的225 rpm,在25毫米振幅(照片和視頻在左邊)或在180 rpm的振動篩上有50毫米振幅(右邊的照片和視頻)。在這兩種條件下,氧傳遞速率大約為30-40毫摩爾O2 L-1 H-1。(對應(yīng)于150-200 h-1的kLa值)
•25毫米振動篩振幅•225轉(zhuǎn)/分
Hydrodynamics inside wells of a 6-square deepwell microplate
For 6-square deepwell microtiter plates, culture volumes between 25 ml and 35 ml are generally used, either at 225 rpm on a shaker with a 25 mm amplitude (photo and video on the left) or at 180 rpm on a shaker with a 50 mm amplitude (photo and video on the right).Under both conditions oxygen transfer rates are approximately 30-40 mmol O2 l-1 h-1 (corresponding to k La values of 150-200 h-1)
•25 mm shaker amplitude•225 rpm
注:楊清辰發(fā)布