Low Profile Perfusion Set 低剖面灌注裝置
用于培養(yǎng)皿的低剖面灌注裝置配有PDI插件和50自粘性

生物相容性墊圈，允許使用常規(guī)35毫米培養(yǎng)皿組裝。

在35mm培養(yǎng)皿中培養(yǎng)的細(xì)胞是一種廣泛應(yīng)用的研究工具，包括

膜片鉗和細(xì)胞內(nèi)離子探針成像。然而，真正的灌注（持續(xù)流入和ou-

解決方案的流）可能很難配置。

Low Profile Perfusion Set 低剖面灌注裝置

Low Profile Perfusion Set 低剖面灌注裝置

用于培養(yǎng)皿的低剖面灌注裝置配有PDI插件和50自粘性

生物相容性墊圈，允許使用常規(guī)35毫米培養(yǎng)皿組裝。

在35mm培養(yǎng)皿中培養(yǎng)的細(xì)胞是一種廣泛應(yīng)用的研究工具，包括

膜片鉗和細(xì)胞內(nèi)離子探針成像。然而，真正的灌注（持續(xù)流入和ou-

解決方案的流）可能很難配置。無外流的藥物輸送需要一種spri型

微注射器，但終導(dǎo)致污染整個盤子后，只有少數(shù)應(yīng)用。這個

迫使科學(xué)家將細(xì)胞貼在蓋玻片上，放入專門設(shè)計的灌注室。

然而，這種轉(zhuǎn)移是一個耗費時間的過程，它會帶來潛在的污染和

額外費用。

PDI室是科學(xué)家經(jīng)過幾年的膜片鉗研究和

培養(yǎng)單細(xì)胞體外灌流培養(yǎng)。這個小室有單獨的開口

傾倒工作艙內(nèi)液位波動并預(yù)防的進(jìn)出口

氣泡進(jìn)入腔室。層流輪廓有助于灌注，并提供更快的解決方案

交換。一個可調(diào)節(jié)的金屬吸管（包括）控制著碟子里的液體水平。密室

設(shè)計用于灌注系統(tǒng)和帶微型球頭的磁性支架，可以

調(diào)節(jié)灌注管?？膳c玻璃底皿配合使用成像。事實上，一些玻璃杯

底皿由標(biāo)準(zhǔn)培養(yǎng)皿制成，例如康寧35毫米?？膳c

溫度控制系統(tǒng)。吸入管需要連接到流出源CFPS-1U

包括三個磁性管/電極支架和不銹鋼吸入管。顯微鏡適配器MA

是必需的。可與磁性和非磁性顯微鏡適配器一起使用。如果與非磁性材料一起使用

適配器，需要一套IMA-MH微型支架。項目#：LPPCP1

培養(yǎng)皿插入物

目錄號說明

用于培養(yǎng)皿的PDI低剖面室插入物

用于培養(yǎng)皿的LPPCP1低剖面灌注裝置

TC-E35x15加熱元件，用于孔徑為15mm的35mm盤子

TC-E35x11加熱元件，適用于孔徑為11mm的35mm盤子

TC-PA-C培養(yǎng)皿適配器，用于康寧和馬特克培養(yǎng)皿

TC-PA-N培養(yǎng)皿適配器，用于Nunc培養(yǎng)皿

TC-PA-W培養(yǎng)皿適配器，用于Willco培養(yǎng)皿

TC-PA-F培養(yǎng)皿適配器，用于氟化物培養(yǎng)皿

TC-PA-G培養(yǎng)皿適配器，用于Greiner Bio One培養(yǎng)皿，玻璃底

 Low Profile Perfusion Set for Petri dishe Comes with PDI insert and 50 selfadhesive
biocompatible gaskets, which allow to use the assembly with regular 35 mm petri dishes.
Cells cultured in 35 mm Petri dishes are a popular research tool used in numerous applications, including
patch clamping and intracellular ion probe imaging. However, true perfusion (continuous inflow and ou -
flow) of solutions can be difficult to configure. Drug delivery without an outflow requires a spri -type
microinjector, but ultimately results in contamination of the entire dish after only a few applications. This
forces scientists to plate cells on cover slips for placement into specially designed perfusion chambers.
However, such transfer is a time consuming process which introduces the potential for contamination plus
additional expense.
The PDI chamber was designed by scientists after several years of patch clamp research combined with
external perfusion of single cells cultured in Petri dishes. The chamber has separate openings for solution
inflow and outflow that dump the fluctuations of the liquid level in the working compartment and preven
bubbles from entering the chamber. The laminar profile facilitates perfusion and provides faster solution
exchange. An adjustable metal suction tube (included) controls the level of liquid in the dish. The chamber
was designed for use with perfusion systems and magnetic holder with miniature ball-joint, which can
accommodate perfusion manifolds. Can be used with glass bottom dishes for imaging. In fact, some glass
bottom dishes are made from standard petri dishes like Corning 35 mm, for example. Can be used with
temperature controlled systems. The suction tubing requires connection to an outflow source CFPS-1U
Includes three magnetic tubing/electrode holders, and stainless suction tubing. A microscope adapter MA
is required. Can be used with both magnetic and non-magnetic microscope adapters. If used with nonmagnetic
adapters, a IMA-MH set of miniature holders is required. Item#: LPPCP1
Petri Dish Inserts
Catalog No. Description
PDI Low Profile Chamber-Insert for Petri Dishes
LPPCP1 Low Profile Perfusion Set for Petri Dishes
TC-E35x15 Heating Element for 35mm dishes with 15mm aperture
TC-E35x11 Heating Element for 35mm dishes with 11mm aperture
TC-PA-C Petri Dish Adapters, for Corning and Mattek petri dishes
TC-PA-N Petri Dish Adapters, for Nunc for Nunc dishes
TC-PA-W Petri Dish Adapters, for Willco dishes
TC-PA-F Petri Dish Adapters, for FluoroDishes
TC-PA-G Petri Dish Adapter, for Greiner Bio-One dishes, glass bottom

 注：楊清辰發(fā)布