性猛交XXXX乱大交派对,四虎影视WWW在线观看免费 ,137最大但人文艺术摄影,联系附近成熟妇女

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊

當前位置:
上海恒遠生物科技有限公司>技術(shù)文章>恒遠產(chǎn)品文獻:多聚甲醛試劑引用文獻

技術(shù)文章

恒遠產(chǎn)品文獻:多聚甲醛試劑引用文獻

閱讀:742          發(fā)布時間:2021-1-11
【文獻標題】Human umbilical cord mesenchymal stem cell-derived exosomes suppress dermal fibroblasts-myofibroblats transition via inhibiting the TGF-β1/Smad 2/3 signaling pathway
【作者】Jian Hu, Yuanwen Chen, Yubin Huang,et.al
【作者單位】深圳市寶安區(qū)人民醫(yī)院(The People’s Hospital of Bao’an Shenzhen)
【文獻中引用產(chǎn)品】
多聚甲醛
【關(guān)鍵詞】Human umbilical cord mesenchymal stem cells,Exosome, TGF-β1/Smad2/3 signaling pathway,Dermal ibroblasts Myofibroblast
【DOI】doi.org/10.1016/j.yexmp.2020.104468
【影響因子(IF)】2.39
出版期刊】《Experimental and Molecular Pathology》
【產(chǎn)品原文引用】
The hUC-MSCs or fibroblasts in exponential phase were seeded at 1 × 105 cells/mL and 3 mL/well in a 6-well plate with a cover glass. After culture for 24 h at 37℃ with 5% CO2, culture medium was discarded, and cells were fixed with paraformaldehyde (Shanghai HengYuan 
Biological Technology Co., Ltd., Shanghai, China) for 10 min. After discarding the fixative solution, the cover glasses were rinsed by PBS 3 times, each for 3 min. PBS was then absorbed with absorbent papers, normal goat serum was dripped on the glass, and fibroblasts were sealed for 30min at room temperature. The sealing liquid was absorbed by absorbent papers without washes. After that, diluted primary antibodies (supplementary Table 1) were dripped into each cover glass, which was incubated in a wet box at 4℃ overnight. Afterwards, the fluorescent secondary antibody goat anti-rat IgG H&L (Alexa Fluor® 488) (1/200, ab150113) was added. Then the glass was dripped with 4',6-diamidino-2-phenylindole (DAPI) for nucleus staining, and sealed with sealing liquid containing anti-fluorescent quenching agent, and then observed under the fluorescence microscope. The above antibodies were all purchased from Abcam Inc. (Cambridge, MA, USA).
完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員!
更多公司福利請關(guān)注“恒遠生物”VX公眾號!

 

收藏該商鋪

登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時間回復(fù)您~

對比框

產(chǎn)品對比 產(chǎn)品對比 聯(lián)系電話 二維碼 意見反饋 在線交流

掃一掃訪問手機商鋪
13636351217
在線留言
太原市| 蕲春县| 化德县| 宝坻区| 板桥市| 开封市| 南投县| 鹤壁市| 峨边| 德安县| 金平| 陵川县| 新昌县| 昭觉县| 恭城| 江西省| 大姚县| 城市| 涿鹿县| 永吉县| 肇源县| 康平县| 嫩江县| 孝义市| 乌鲁木齐县| 乐平市| 任丘市| 唐山市| 香格里拉县| 灵台县| 威信县| 汉寿县| 资兴市| 五常市| 兴安县| 辽宁省| 尼木县| 武清区| 海安县| 南昌市| 南乐县|