性猛交XXXX乱大交派对,四虎影视WWW在线观看免费 ,137最大但人文艺术摄影,联系附近成熟妇女

產品展廳收藏該商鋪

您好 登錄 注冊

當前位置:
上海恒遠生物科技有限公司>技術文章>恒遠產品文獻:多聚甲醛試劑引用文獻

技術文章

恒遠產品文獻:多聚甲醛試劑引用文獻

閱讀:730          發(fā)布時間:2021-1-11
【文獻標題】Human umbilical cord mesenchymal stem cell-derived exosomes suppress dermal fibroblasts-myofibroblats transition via inhibiting the TGF-β1/Smad 2/3 signaling pathway
【作者】Jian Hu, Yuanwen Chen, Yubin Huang,et.al
【作者單位】深圳市寶安區(qū)人民醫(yī)院(The People’s Hospital of Bao’an Shenzhen)
【文獻中引用產品】
多聚甲醛
【關鍵詞】Human umbilical cord mesenchymal stem cells,Exosome, TGF-β1/Smad2/3 signaling pathway,Dermal ibroblasts Myofibroblast
【DOI】doi.org/10.1016/j.yexmp.2020.104468
【影響因子(IF)】2.39
出版期刊】《Experimental and Molecular Pathology》
【產品原文引用】
The hUC-MSCs or fibroblasts in exponential phase were seeded at 1 × 105 cells/mL and 3 mL/well in a 6-well plate with a cover glass. After culture for 24 h at 37℃ with 5% CO2, culture medium was discarded, and cells were fixed with paraformaldehyde (Shanghai HengYuan 
Biological Technology Co., Ltd., Shanghai, China) for 10 min. After discarding the fixative solution, the cover glasses were rinsed by PBS 3 times, each for 3 min. PBS was then absorbed with absorbent papers, normal goat serum was dripped on the glass, and fibroblasts were sealed for 30min at room temperature. The sealing liquid was absorbed by absorbent papers without washes. After that, diluted primary antibodies (supplementary Table 1) were dripped into each cover glass, which was incubated in a wet box at 4℃ overnight. Afterwards, the fluorescent secondary antibody goat anti-rat IgG H&L (Alexa Fluor® 488) (1/200, ab150113) was added. Then the glass was dripped with 4',6-diamidino-2-phenylindole (DAPI) for nucleus staining, and sealed with sealing liquid containing anti-fluorescent quenching agent, and then observed under the fluorescence microscope. The above antibodies were all purchased from Abcam Inc. (Cambridge, MA, USA).
完整版PDF文獻請咨詢在線客服或者聯系我司業(yè)務員!
更多公司福利請關注“恒遠生物”VX公眾號!

 

收藏該商鋪

登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時間回復您~

對比框

產品對比 產品對比 聯系電話 二維碼 意見反饋 在線交流

掃一掃訪問手機商鋪
13636351217
在線留言
渭南市| 准格尔旗| 马边| 横山县| 黄浦区| 满城县| 临朐县| 开封市| 璧山县| 道孚县| 巫山县| 淮安市| 花莲县| 蒲江县| 屯留县| 亚东县| 青田县| 镇康县| 上栗县| 怀安县| 汝阳县| 南木林县| 根河市| 浠水县| 二连浩特市| 荃湾区| 宝应县| 防城港市| 酒泉市| 武山县| 张家川| 汾阳市| 土默特左旗| 休宁县| 黑龙江省| 安陆市| 哈尔滨市| 镶黄旗| 盈江县| 洛阳市| 山东省|