性猛交XXXX乱大交派对,四虎影视WWW在线观看免费 ,137最大但人文艺术摄影,联系附近成熟妇女

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊(cè)

當(dāng)前位置:
上海恒遠(yuǎn)生物科技有限公司>技術(shù)文章>恒遠(yuǎn)產(chǎn)品文獻(xiàn):多聚甲醛試劑引用文獻(xiàn)

技術(shù)文章

恒遠(yuǎn)產(chǎn)品文獻(xiàn):多聚甲醛試劑引用文獻(xiàn)

閱讀:775          發(fā)布時(shí)間:2021-1-11
【文獻(xiàn)標(biāo)題】Human umbilical cord mesenchymal stem cell-derived exosomes suppress dermal fibroblasts-myofibroblats transition via inhibiting the TGF-β1/Smad 2/3 signaling pathway
【作者】Jian Hu, Yuanwen Chen, Yubin Huang,et.al
【作者單位】深圳市寶安區(qū)人民醫(yī)院(The People’s Hospital of Bao’an Shenzhen)
【文獻(xiàn)中引用產(chǎn)品】
多聚甲醛
【關(guān)鍵詞】Human umbilical cord mesenchymal stem cells,Exosome, TGF-β1/Smad2/3 signaling pathway,Dermal ibroblasts Myofibroblast
【DOI】doi.org/10.1016/j.yexmp.2020.104468
【影響因子(IF)】2.39
出版期刊】《Experimental and Molecular Pathology》
【產(chǎn)品原文引用】
The hUC-MSCs or fibroblasts in exponential phase were seeded at 1 × 105 cells/mL and 3 mL/well in a 6-well plate with a cover glass. After culture for 24 h at 37℃ with 5% CO2, culture medium was discarded, and cells were fixed with paraformaldehyde (Shanghai HengYuan 
Biological Technology Co., Ltd., Shanghai, China) for 10 min. After discarding the fixative solution, the cover glasses were rinsed by PBS 3 times, each for 3 min. PBS was then absorbed with absorbent papers, normal goat serum was dripped on the glass, and fibroblasts were sealed for 30min at room temperature. The sealing liquid was absorbed by absorbent papers without washes. After that, diluted primary antibodies (supplementary Table 1) were dripped into each cover glass, which was incubated in a wet box at 4℃ overnight. Afterwards, the fluorescent secondary antibody goat anti-rat IgG H&L (Alexa Fluor® 488) (1/200, ab150113) was added. Then the glass was dripped with 4',6-diamidino-2-phenylindole (DAPI) for nucleus staining, and sealed with sealing liquid containing anti-fluorescent quenching agent, and then observed under the fluorescence microscope. The above antibodies were all purchased from Abcam Inc. (Cambridge, MA, USA).
完整版PDF文獻(xiàn)請(qǐng)咨詢?cè)诰€客服或者聯(lián)系我司業(yè)務(wù)員!
更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物”VX公眾號(hào)!

 

收藏該商鋪

請(qǐng) 登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

對(duì)比框

產(chǎn)品對(duì)比 產(chǎn)品對(duì)比 聯(lián)系電話 二維碼 意見反饋 在線交流

掃一掃訪問手機(jī)商鋪
13636351217
在線留言
五河县| 定襄县| 凤翔县| 永登县| 江津市| 衡水市| 玉环县| 隆昌县| 尖扎县| 镇沅| 神木县| 盖州市| 宁阳县| 乐昌市| 九寨沟县| 双柏县| 宝鸡市| 绥宁县| 东方市| 望城县| 徐闻县| 东明县| 巢湖市| 舒兰市| 英吉沙县| 仲巴县| 孝感市| 仙居县| 临洮县| 滕州市| 汽车| 咸丰县| 碌曲县| 句容市| 碌曲县| 兰溪市| 葵青区| 庆安县| 佳木斯市| 博兴县| 津市市|